Modulatory roles of ergothioneine on heat shock protein-70, tumour necrosis factor-alpha, and rectal temperatures of Arabian stallions following race of 2000 m in a hot-dry environment

Document Type : Research Article

Authors

1 Department of Veterinary Physiology and Biochemistry, University of Ilorin, P. M. B. 1515, Ilorin, Nigeria.

2 Department of Veterinary Physiology, Ahmadu Bello University, P. M. B. 1044, Samaru- Zaria, Nigeria.

3 National Animal Production Research Institute, P. M. B. 1096, Shika-Zaria, Nigeria.

4 Department of Veterinary Medicine, University of Ilorin, P.M.B. 1515, Ilorin, Nigeria.

Abstract

Experiments were performed to determine the effect of ergothioneine on rectal temperature and the serum concentrations of heat shock protein-70 (HSP-70) and tumor necrosis factor-α (TNF-α) in stallions following a race of 2000 m in a hot-dry environment. Eighteen stallions weighing approximately 400 kg each were used for the experiment. They were divided into three groups of six stallions each. Group I (EEX) was the experimental group that was administered ergothioneine (0.5 mg/kg per os), while group II (EEC) did not receive ergothioneine before exercise. The third group (EEN) was neither administered ergothioneine nor exercised. The dry-bulb temperature and the relative humidity of the experiment were determined for six days and on the day of the experiment. The temperature-humidity index was also calculated. Rectal temperature, serum HSP-70, and TNF-α concentrations of all horses were measured before commencement, immediately after, and 2 h after the exercise. The dry-bulb temperature and relative humidity which showed diurnal fluctuations increased significantly (p < 0.05) between 06.00 h and 12.00 h (22.6 ± 1.23 and 38.6 ± 6.5, respectively). Serum TNF-α and HSP-70 levels of the stallions in the EEX group were higher than the values obtained in the EEC and EEN groups (p < 0.05). The values of rectal temperature obtained were lower (p < 0.05) in the EEX group than in the other groups. Therefore, it could be concluded that ergothioneine modulated rectal temperature, as well as TNF-α and HSP-70 concentrations in the stallions, and might be beneficial to horses during exercise.

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Volume 14, Issue 4 - Serial Number 29
This issue XML file is being prepared.
November 2022
Pages 19-25
  • Receive Date: 25 May 2022
  • Revise Date: 02 September 2022
  • Accept Date: 03 September 2022
  • First Publish Date: 10 September 2022