Evaluation of Egg Drop Syndrome Virus Fiber Protein as a Vaccine Candidate: In Silico Analysis, Expression, Purification and Its Stability

Articles in Press

Document Type : Research Article

Authors

• Javad Najafian ¹
• Abasalt Hosseinzadeh Colagar ²
• Ali Mostafaie ³

¹ Department of Molecular and Cell Biology, Faculty of Science, University of Mazandaran, Babolsar

² Department of Molecular and Cell Biology, Faculty of Science, University of Mazandaran, Babolsar, PC: 47416-95447, Mazandaran, Iran.

³ Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

10.22067/ijvst.2025.93948.1564

Abstract

The egg drop syndrome virus (EDSV), an avian adenovirus, triggers a sharp decline in both egg production and quality in infected chickens, resulting in significant economic impacts for the poultry sector. Based on previous studies, EDSV fiber protein could be a candidate for subunit vaccine against EDSV. Present research focuses on the expression, purification, and thermal stability evaluation of recombinant fiber protein as a vaccine against EDSV. In an in silico approach, we investigated the fiber protein structure and expressed it in Escherichia coli (E. coli) as a vaccine candidate. We also evaluated the thermal stability of the expressed protein. The protein was expressed predominantly as soluble trimeric proteins in E. coli and expression level after nickel-affinity purification was 15 mg/L. Structural analysis using immunological and bioinformatics tools Confirmed retention of the native trimeric conformation in the recombinant protein. Based on the thermal stability evaluation on this recombinant protein, the protein showed good thermal stability, which can be a good candidate for a vaccine against EDSV.

Keywords

• Egg drops syndrome virus
• Fiber protein
• In silico
• Vaccines

Main Subjects

• Avian diseases

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