TTILE: BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF Pseudomonas putida, P. fluorescens and P. aeruginosa ISOLATES FROM Oreochromis niloticus (TILAPIA ) AND Clarias gariepinus (AFRICAN CATFISH)

Articles in Press

Document Type : Research Article

Authors

• Mannir Dahiru Usman ¹
• Clarance Suh YAH ²
• Victoria Folakemi Akinjogunla ³
• Tuaheed Abubakar Muazu ⁴
• Abdulrazak Lawal ⁵
• Yusuf Abubakar ⁵
• Khalid Shuaibu HASSAN ⁶
• Jamiu Olatoye Salako ⁴
• Christian Anuoluwapo OLASENI ³

¹ Department of Veterinary Medicine, Bayero University Kano Nigeria

² 2. Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa

³ 3. Department of Fisheries and Aquaculture, Bayero University Kano, Nigeria

⁴ 4. Department of Veterinary Anatomy, Bayero University Kano, Nigeria

⁵ 5. Department of Veterinary Public health and Preventive Medicine, Bayero University Kano, Nigeria

⁶ 6. Centre for Dryland Agriculture, Bayero University Kano

10.22067/ijvst.2025.90677.1437

Abstract

ABSTRACT

Bacterial pathogens cause high economic losses in the fish farming industry and seriously threaten public health. This study described the phenotypic and the genomic characteristics of pathogenic Pseudomonas species in two finfishes (Oreochromis niloticus and Clarias gariepinus) from the Galadima fish market in Kano metropolis Nigeria. Twenty fish samples, including 10 Tilapia and 10 African catfish were randomly obtained and their liver, spleen, intestine and gills were collected. Pseudomonas spp. was screened via culturing and isolation techniques and biochemical tests. The molecular characterization of the isolates was carried out based on 16S rRNA gene sequence analysis. A total of 6 % Pseudomonas spp. isolates were confirmed as P. putida (5 %), P. aeruginosa (1.25 %), and P. fluorescens (1.25 %). This study confirms the presence of potential pathogenic Pseudomonas species in commercially important finfish species from the Galadima fish market in Kano, Nigeria. These findings highlight the need for regular monitoring and molecular surveillance of bacterial pathogens in aquaculture to mitigate economic losses and reduce potential public health risks associated with fish consumption.

Keywords

• Tilapia
• African catfish
• Sequencing
• Bacteria

Main Subjects

• Aquatic animal diseases

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