Document Type : Research Article
Authors
1
MSc, Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
2
Associate Professor, Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
3
Department of Anatomy and Histology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq
10.22067/ijvst.2025.89476.1411
Abstract
The extensive application of carbendazim (Carb: methyl-2-benzimidazole carbamate) in agricultural and veterinary contexts raises significant environmental issues due to its residues, which interfere with spermatogenesis. Recent studies have highlighted the health benefits of coenzyme Q10 (CoQ10) supplementation, primarily linked to its anti-apoptotic and anti-inflammatory effects. Consequently, this study aimed to explore the potential mechanistic pathways through which CoQ10 may alleviate Carb-induced reproductive dysfunction in rats. Adult male Wistar rats were subjected to Carb (150 mg/kg orally) either alone or in conjunction with CoQ10 (200 mg/kg) over a period of nine weeks. At the end of the period, testicular tissues collected for histological analysis, immunohistochemical evaluation, hormonal measurement, and molecular analysis. The Carb-treated group displayed significant testicular damage, as evidenced by immunostaining results, which indicated a marked increase in the expression of bax and caspase-3, alongside a significant decrease in bcl-2 protein immunopositivity within the testes. The findings indicated that Carb exposure led to a reduction in miR-202-5p expression, which was concurrent with decreased levels of testosterone and luteinizing hormone. In contrast, the administration of CoQ10 alongside Carb treatment resulted in the restoration of testicular architecture, hormonal equilibrium, and a reduction in the apoptotic index, bringing these parameters close to those observed in the control group. Furthermore, the Carb + CoQ10 group exhibited elevated levels of miR-202-5p, with testicular tissues showing a near-normal histological structure. In summary, Carb induces detrimental changes in testicular function through the miR-202-5p/apoptosis pathway, while CoQ10 demonstrates potential efficacy in counteracting the harmful effects attributed to Carb.
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