Therapeutic Intervention for Caseous Lymphadenitis Using Intra-abscess Instillation of Ozone or Hydrogen Peroxide in Small Ruminants

Document Type : Research Article


1 Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Pharmaceutical control, Faculty of pharmacy, Mashhad university of medical sciences, Mashhad, Iran.

3 Drs. Norouzi's private veterinary practice, Zirkuh District, South Khorasan province, Iran.

4 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.


CLA is an economically and zoonotically important disease in the world. The lack of a therapeutic procedure limits the treatment mainly to surgical intervention. Therapeutic efficacies of the intra-abscess instillation of O3-oil and H2O2-gly in CLA in small ruminants were tested. One hundred eighty affected sheep and goats were allocated to five groups as follows: 1) NC (no intervention), 2) PC1 (injection of olive oil), 3) PC2 (injection of glycerin), 4) injection of O3-oil, 5) injection of H2O2-gly. Samples of abscess contents were collected for microbiological examination prior to injection. The VAs were measured on T0, then with two-week intervals on T1 and T2. On T0 and T2, VAs were as NC (2.9 ± 0.5; 3.5 ± 0.5), PC1 (3.4 ± 0.7; 6.6 ± 1), PC2 (3.1 ± 0.7; 3.3 ± 0.9), O3-oil (3.3 ± 0.4; 0.4 ± 0.4), and H2O2-gly (4.6 ± 0.4, 1.5 ± 0.4). Statistical analysis showed a significant decrease in VAs, merely in treatment groups. CP was recovered in 48.3% of bacteriological samples. The results of this study suggested that O3-oil and H2O2-gly would be reliable therapeutic agents for treating and controlling CLA. Ozone showed apparently a higher  efficacy and caused more rapid shrinkage/recession of the abscesses, compared to hydrogen peroxide..

Graphical Abstract

Therapeutic Intervention for Caseous Lymphadenitis Using Intra-abscess Instillation of Ozone or Hydrogen Peroxide in Small Ruminants


Main Subjects

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Volume 15, Issue 3 - Serial Number 32
This issue XML file is being prepared.
September 2023
Pages 16-26
  • Receive Date: 25 April 2023
  • Revise Date: 26 July 2023
  • Accept Date: 31 July 2023
  • First Publish Date: 01 September 2023