Serial blood gas analysis during fluid resuscitation of hypovolemic dogs

Document Type : Research Article


1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran.

4 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.


It has been documented that hemodynamic disturbances occur in hypovolemic patients. Therefore, the early management of hypovolemia is essential to achieve optimal outcomes. Blood gas, which changes rapidly during hemodynamic instability, can be used as a diagnostic approach for monitoring emergency patients. The objective of the current study was to evaluate the results of resuscitation with hydroxyethyl starch (HES) or lactated Ringer's solution (LR) on venous and arterial blood gas. In addition, the difference between venous and arterial blood gas parameters is investigated to assess the possibility of using venous blood gas analysis as a successor for arterial blood gas analysis in the resuscitation of hypovolemic dogs. Venous and arterial pH, PO2, PCO2, HCO3-, and base excess were analyzed at the end of each study stage as follow: 1) Establishment of anesthesia, 2) Blood collection to an arterial mean pressure of 40-50 mm Hg, 3) Maintaining dogs in a hypovolemic state, 4) Resuscitation with LR (group A) or HES (group B) in four steps, and 5) One hour after the final resuscitation step. Hypovolemia decreased the studied parameters, except venous PCO2, which showed a significant increase (p < 0.05). Fluid resuscitation returned the studied parameters to the control values as venous PCO2 in group A and HCO3- in group B showed a significant change in comparison with the control values (p < 0.05). We found that venous pH, HCO3-, and PCO2 can be used as less invasive and safer alternatives to similar arterial parameters to monitor the fluid resuscitation of hypovolemic dogs.


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1.    Cortés-Samacá CA, Meléndez-Flórez HJ, Robles SA, Meléndez-Gómez EA, Puche-Cogollo CA, Mayorga-Anaya HJ. Base deficit, lactate clearance, and shock index as predictors of morbidity and mortality in multiple-trauma patients. Colombian Journal of Anestesiology. 2018;46(3):208-15.
2.    Hashemi SJ, Heidari SM, Yaraghi A, Seirafi R. Acid–base and hemodynamic status of patients with intraoperative hemorrhage using two solution types: Crystalloid Ringer lactate and 1.3% sodium bicarbonate in half‑normal saline solution. Advanced Biomedical Research. 2016;28(5):190.
3.    Sánchez-Díaz JS, Peniche-Moguel KG, Rivera-Solís G, Martínez-Rodríguez EA, Del-Carpio-Orantes L, Pérez-Nieto OR, et al. Hemodynamic monitoring with two blood gases: “a tool that does not go out of style”. Colombian Journal of Anestesiology. 2020; 49(1):e928.
4.    Kuleš J, Brkljačić M, Crnogaj M, Potočnjak D, Grden D, Torti M, et al. Arterial blood acid-base and electrolyte values in dogs: conventional and “strong ion” approach. Veterinarski Arhiv. 2015;85(5):533-45.
5.    Tamura J, Itami T, Ishizuka T, Fukui S, Miyoshi K, Sano T, et al. Central venous blood gas and acid-base status in conscious dogs and cats. The Journal of Veterinary Medical Science. 2015;77(7):865–69.
6.    Rose R, Kheirabadi BS, Klemcke HG. Arterial blood gases, electrolytes, and metabolic indices associated with hemorrhagic shock: inter- and intrainbred rat strain variation. Journal of Applied Physiology. 2013;114(9):1165-73.
7.    Williams KB, Christmas AB, Heniford BT, Fong Sing R, Messick J. Arterial vs venous blood gas differences during hemorrhagic shock. World Journal of Critical Care Medicine. 2014; 3(2):55-60.
8.    Balmaceda A, Arora S, Sondheimer I, Hollon MM. Resuscitation from a pH of 6.5: A Case Report and Review of Pathophysiology and Management of Extreme Acidosis from Hypovolemic Shock after Trauma. Journal of Trauma and Injury. 2019;32(4):238-42.
9.    Ghvaladze M, Chikhladze R, Sulaberidze G, Didava G, Gegeshidze T, Chkhaidze M, et al. Arterial and Venous Blood Gas Monitoring in Hemorrhagic Shock. Bulletin of the Georgian National Academy of Sciences. 2011;5(2):127-32.
10.    Scheiner B, Lindner G, Reiberger T, Schneeweiss B, Trauner M, Zauner C, et al. Acid-base disorders in liver disease. Journal of Hepatology. 2017;67(5):1062-73.
11.    Oropello JM, Manasia A, Hannon E, Leibowitz A, Benjamin E. Continuous Fiberoptic Arterial and Venous Blood Gas Monitoring in Hemorrhagic Shock. Chest. 1996;109(4):1049-55.
12.    Mazzaferro E, Powell LL. Fluid therapy for the emergent small animal patient: crystalloids, colloids, and albumin products. Veterinary Clinics of North America: Small Animal Practice. 2013;43(4):721-34.
13.    Bae J, Soliman M, Kim H, Kang S, Kim W, Ahn S, et al. Rapid exacerbation of renal function after administration of hydroxyethyl starch in a dog. The Journal of Veterinary Medical Science. 2017;79(9):1591–95.
14.    Treger R, Pirouz S, Kamangar N, Corry D. Agreement between central venous and arterial blood gas measurements in the intensive care unit. Clinical Journal of the American Society of Nephrology. 2010;5(3):390-94.
15.    Kelly AM. Review article: Can venous blood gas analysis replace arterial in emergency medical care. Emergency Medicine Australasia. 2010;22(6):493–98.
16.    Rudkin SE, Anderson CL, Grogan TR, Elashoff DA, Treger RM. Assessing Acid–Base Status in Circulatory Failure: Relationship Between Arterial and Peripheral Venous Blood Gas Measurements in Hypovolemic Shock. Journal of Intensive Care Medicine. 2020;35(5):511-18.
17.    Kellum J. Fluid resuscitation and hyperchloremic acidosis in experimental sepsis: Improved short-term survival and acid-base balance with Hextend compared with saline. Critical Care Medicine. 2002;30(2):300-05.
18.    Azargoun R, Avizeh R, Ghadiri A, Imani Rastabi H, Pourmahdi Borujeni M. Ultrasonographic assessment of caudal vena cava to aorta ratio as a novel endpoint in hemorrhagic shock resuscitation in dogs. Iranian Journal of Veterinary Surgery. 2019;14(2):134-41.
Volume 14, Issue 4 - Serial Number 29
This issue XML file is being prepared.
November 2022
Pages 10-18
  • Receive Date: 25 April 2022
  • Revise Date: 02 August 2022
  • Accept Date: 26 August 2022
  • First Publish Date: 28 August 2022