Serological diagnosis and risk factors associated with bovine paratuberculosis in the municipality of Tuta, Colombia

Document Type : Research Article

Authors

Grupo de Investigación en Medicina Veterinaria y Zootecnia - GIDIMEVETZ, Universidad Pedagógica y Tecnológica de Colombia, Colombia.

Abstract

Bovine paratuberculosis or Johne's disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP), which affects domestic and wild ruminants around the world. The clinical presentation of MAP infection is characterized by chronic diarrhea unresponsive to treatment. The objective of the present study was to investigate the seroprevalence and risk factors associated with bovine paratuberculosis in cattle herds of Tuta, Boyacá, Colombia. This descriptive cross-sectional study with simple random sampling was performed on 882 blood samples taken from cattle of different racial and age groups. Blood samples were processed using an indirect enzyme-linked immunosorbent assay (PARACHEK® 2 Kit, Prionics AG, Switzerland). The obtained data were analyzed by the statistical software Epi Info. In this study, a general seroprevalence of 3.1% was found, and seropositivity in females was 3.6%. The highest prevalence of antibodies against MAP was in individuals > 4 years (5%) and the Jersey breed (4.8%). Therefore, the age of over 4 years was identified as a risk factor associated with MAP. Moreover, a statistical association was found between management and biosafety variables, such as pen management (p = 0.012), feeding with concentrate (p = 0.012), and the presence of diarrhea on the farm (p = 0.048). It could be concluded that the disease is present in Tuta, however, considering factors, such as the chronicity of the disease and the diagnostic method used, it is expected that the number of infected animals is much higher than presented in this research.

Keywords


1.     Fecteau ME. Paratuberculosis in Cattle. Vet. Clin. N. Am. – Food Anim. Pract. 2018;34(1):209–22. 
2.     Nielsen SS, Toft N. Ante mortem diagnosis of paratuberculosis: A review of accuracies of ELISA, interferon-γ assay and faecal culture techniques. Vet. Microbiol. 2008;129(3–4):217–35. 
3.     Sweeney RW. Pathogenesis of Paratuberculosis. Vet. Clin. N. Am. – Food Anim. Pract. 2011;27(3):537–46. 
4.     Garcia AB, Shalloo L. Invited review: The economic impact and control of paratuberculosis in cattle. Int. J. Dairy Sci. 2015;98(8):5019–39. 
5.     Pourmahdi Borujeni M, Haji Hajikolaei MR, Ghorbanpoor M, Elhaei Sahar H, Bagheri S, Roveyshedzadeh S. Comparison of Mycobacterium avium subsp. paratuberculosis infection in cattle, sheep and goats in the Khuzestan Province of Iran: Results of a preliminary survey. Vet. Med. Sci. 2021;7(5):1970–9. 
6.     Rasmussen P, Barkema HW, Mason S, Beaulieu E, Hall DC. Economic losses due to Johne’s disease (paratuberculosis) in dairy cattle. Int. J. Dairy Sci.  2021; 104(3): 3123-43. 
7.     Bannantine JP, Bermudez LE. No holes barred: Invasion of the intestinal mucosa by mycobacterium avium subsp. paratuberculosis. Infect. Immun. 2013;81(11):3960–5. 
8.     de Lacerda Roberto JP, Limeira CH, da Costa Barnabé NN, Soares RR, Silva MLCR, de Barros Gomes AA, et al. Antibody detection and molecular analysis for Mycobacterium avium subspecies paratuberculosis (MAP) in goat milk: Systematic review and meta-analysis. Res. Vet. Sci. 2021; 135:72–7.
9.     Correa-Valencia N, García-Tamayo YM, Fernández-Silva JA. Mycobacterium avium subsp. Paratuberculosis in Colombia (1924-2016): A review. RCCP. 2018;31(3):165–79. 
10.     OIE. Paratuberculosis [Internet]. 2020 [cited 2022 Apr 25]. Available from: https://www.oie.int/es/sanidad-animal-en-el-mundo/enfermedades-de-los-animales/paratuberculosis/
11.     Bulla-Castañeda DM, Díaz-Anaya AM, Garcia-Corredor DJ, Pulido-Medellín MO. Serodiagnóstico de Paratuberculosis en bovinos del municipio de Sogamoso, Boyacá (Colombia). Entramado. 2020;16(2):312–20. 
12.     Velez M, Rendón Y, Valencia A, Ramirez N, Fernández J. Seroprevalencia de Mycobacterium avium Subsp. paratuberculosis (MAP) en una granja de ganado de carne de bosque húmedo tropical en Caucasia, Antioquia, Colombia. RECIA. 2016 1;8(2):167–76. 
13.     Jaramillo-Moreno S, Montoya-Zuluaga MA, Uribe-Santa JS, Ramírez-Vásquez NF, Fernández-Silva JA. Seroprevalencia de paratuberculosis (Mycobacterium avium subsp. paratuberculosis) en un hato de lechería especializada del altiplano norte de Antioquia, Colombia. Rev. Med. Vet. Zoot. 2017;11(2):24–33. 
14.     Constable PD, Hinchcliff KW, Done SH, Grünberg W. Veterinary Medicine. 11th ed. Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier; 2017. 2308 p. 
15.     Borujeni MP, Hajikolaei MRH, Ghorbanpoor M, Sahar HE, Bagheri S, Roveyshedzadeh S. Comparison of Mycobacterium avium subsp. paratuberculosis infection in cattle, sheep and goats in the Khuzestan Province of Iran: Results of a preliminary survey. Vet. Med. Sci. 2021;7(5):1970–9. 
16.     Elmagzoub WA, Adam NM, Idris SM, Mukhtar ME, Abdelaziz SA, Okuni JB, et al. Seroprevalence of Mycobacterium avium subsp. paratuberculosis in Dairy Cattle in Khartoum State, Sudan. Vet. Sci. 2020;7(4):209. 
17.     Ozsvari L, Lang Z, Monostori A, Kostoulas P, Fodor I. Bayesian estimation of the true prevalence of paratuberculosis in Hungarian dairy cattle herds. Prev. Vet. Med. 2020; 183:105124. 
18.     Abdala A, Aguirre N, Luca E, Storani G, Storero R, Torioni de Echaide S. Prevalence of paratuberculosis in dairy and beef catlle in two department of Santa Fe province (Argentina). INVET. 2019;21(1):17–26. 
19.     Sosa P, Alvarado M, Traveria G. ELISA indirecto para el diagnóstico de paratuberculosis en bovinos de tambo utilizando pooles de muestras de leche.Rev. med. vet. 2021;102(2):35–40. 
20.     Verdugo C, Valdes MF, Salgado M. Herd level risk factors for Mycobacterium avium subsp. paratuberculosis infection and clinical incidence in dairy herds in Chile. Prev. Vet. Med. 2020; 176:104888. 
21.     Vilar ALT, Santos CSAB, Pimenta CLRM, Freitas TD, Brasil AWL, Clementino IJ, et al. Herd-level prevalence and associated risk factors for Mycobacterium avium subsp. paratuberculosis in cattle in the State of Paraíba, Northeastern Brazil. Prev. Vet. Med 2015;121(1–2):49–55. 
22.     Doria - Ramos M, Oviedo M, Oviedo T, Canabal M, Pérez J, Fernández J. Seroprevalencia a Mycobacterium avium subsp. paratuberculosis en bovinos criollos colombianos Romosinuano y costeno con cuernos. Revi. MVZ Cordoba. 2020;25(2): e1611. 
23.     Feola RP, Collins MT, Czuprynski CJ. Hormonal modulation of phagocytosis and intracellular growth of Mycobacterium aviums subsp.paratuberculosis in bovine peripheral blood monocytes. Microb. Pathog. 1999;26(1):1–11. 
24.     Alhussien MN, Dang AK. Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review. Vet. World. 2020;13(9):1848. 
25.     Suarez ALP, López-Rincón G, Martínez Neri PA, Estrada-Chávez C. Prolactin in Inflammatory Response. Adv. Exp. Med. Biol. 2015; 846:243–64. 
26.     Hole N, Maclay M. The diagnosis of Johne’s disease in cattle and the identification of Mycobacterium johnei infection. Vet. Rec. Open. 1959; 71:1145–8. 
27.     Robinson MW, Donnelly Sheila, Hutchinson AT, To Joyce, Taylor NL, Norton RS, et al. A Family of Helminth Molecules that Modulate Innate Cell Responses via Molecular Mimicry of Host Antimicrobial Peptides. Wynn TA, editor. PLoS Pathogens. 2011;7(5): e1002042. 
28.     Sorge US, Lissemore K, Godkin A, Hendrick S, Wells S, Kelton D. Associations between paratuberculosis milk ELISA result, milk production, and breed in Canadian dairy cows. Int. J. Dairy Sci. 2011;94(2):754–61. 
29.     Kiser JN, White SN, Johnson KA, Hoff JL, Taylor JF, Neibergs HL. Identification of loci associated with susceptibility to Mycobacterium avium subspecies paratuberculosis (Map) tissue infection in cattle. Sci. J. Anim. Sci. 2017;95(3):1080–91. 
30.     Kirkpatrick B, Shook G. Genetic susceptibility to paratuberculosis. Vet Clin North Am Food Anim Pract. 2011;27(3):559–71. 
31.     Elzo MA, Rae DO, Lanhart SE, Wasdin JG, Dixon WP, Jones JL. Factors associated with ELISA scores for paratuberculosis in an Angus-Brahman multibreed herd of beef cattle. Sci. J. Anim. Sci. 2006 ;84(1):41–8. 
32.     Salgado M, Steuer P, Troncoso E, Collins M. Evaluation of PMS-PCR technology for detection of Mycobacterium avium subsp. paratuberculosis directly from bovine fecal specimens. Vet Microbiol. 2013;167(3–4):725–8. 
33.     Nielsen SS, Toft N, Okura H. Dynamics of Specific Anti-Mycobacterium avium Subsp. paratuberculosis Antibody Response through Age. PLoS One;8(4):e63009. 
34.     Nielsen S, Ersbøll A. Age at Occurrence of Mycobacterium avium Subspecies paratuberculosis in Naturally Infected Dairy Cows. Int. J. Dairy Sci. 2006;89(12):4557–66. 
35.     Mitchel R, Medley G, Collins M, Schukken Y. A meta-analysis of the effect of dose and age at exposure on shedding of Mycobacterium avium subspecies paratuberculosis (MAP) in experimentally infected calves and cows.     Epidemiol. Infect. 2012; 140(2):231–46. 
36.     Sweeney RW, Whitlock RH, Buckley CL, Spencer PA. Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Paratuberculosis in Dairy Cattle. J Vet Diagn Invest. 1995;7(4):488–93. 
37.     Woodbine KA, Schukken YH, Green LE, Ramirez-Villaescusa A, Mason S, Moore SJ, et al. Seroprevalence and epidemiological characteristics of Mycobacterium avium subsp. paratuberculosis on 114 cattle farms in south west England. Prev. Vet. Med. 2009;89(1–2):102–9. 
38.     Benavides B, Arteaga -Cadena A V, Montezuma Misnaza CA. Estudio epidemiológico de paratuberculosis bovina en hatos lecheros del sur de Nariño, Colombia. Rev. Med. Vet. Zoot. 2016;(31):57–66. 
39.     Hussain SM, Javed MT, Rehman AU, Rizvi F, Qamar M. Prevalence of paratuberculosis in cattle and buffaloes in Punjab Pakistan. Pak. J. Agric. Sci. 2018;55(2):427–32. 
40.     Correa-Valencia NM, Ramírez NF, Arango-Sabogal JC, Fecteau G, Fernández-Silva JA. Prevalence of Mycobacterium avium subsp. paratuberculosis infection in dairy herds in Northern Antioquia (Colombia) and associated risk factors using environmental sampling. Prev. Vet. Med. 2019; 170:104739. 
41.     Correa-Valencia N, Arango-Lezcano F, Fernández-Silva J. Mycobacterium avium subsp. paratuberculosis antibodies in cows of low-tropic dairy herds in Colombia. Rev. MVZ Córdoba. 2020;25(2):e1782. 
42.     Eisenberg SWF, Nielen M, Hoeboer J, Rutten V, Heederik D, Koets AP. Environmental contamination with Mycobacterium avium subspecies paratuberculosis within and around a dairy barn under experimental conditions. Int. J. Dairy Sci. 2012;95(11):6477–82. 
43.     Tavornpanich S, Johnson WO, Anderson RJ, Gardner IA. Herd characteristics and management practices associated with seroprevalence of Mycobacterium avium subsp paratuberculosis infection in dairy herds. Am. J. Vet. Res. 2008;69(7):904–11. 
44.     Eisenberg S, Nielen M, Hoeboer J, Bouman M, Heederik D, Koets A. Mycobacterium avium subspecies paratuberculosis in bioaerosols after depopulation and cleaning of two cattle barns. Vet. Rec. Open. 2011;168(22):587–587. 
45.     Cheng Z, Liu M, Wang P, Liu P, Chen M, Zhang J, et al. Characteristics and Epidemiological Investigation of Paratuberculosis in Dairy Cattle in Tai’an, China. Biomed Res. Int.2020;2020. 
46.     Tütüncü M, Kiliçoğlu Y, Güzel M, Pekmezci D, Gülhan T. Seropositivity of Mycobacterium paratuberculosis in Cattle with Chronic Diarrhea in the Middle Black Sea Region. Ataturk Univ. Vet. Bilim. Derg. 2018;13(1):1–5. 
47.     Corbett CS, Naqvi SA, de Buck J, Kanevets U, Kastelic JP, Barkema HW. Environmental sample characteristics and herd size associated with decreased herd-level prevalence of Mycobacterium avium ssp. paratuberculosis. Int. J. Dairy Sci.2018;101(9):8092–9. 
48.     Wolf R, Barkema HW, de Buck J, Orsel K. Dairy farms testing positive for Mycobacterium avium ssp. paratuberculosis have poorer hygiene practices and are less cautious when purchasing cattle than test-negative herds. Int. J. Dairy Sci. 2016;99(6):4526–36. 
49.     Barkema HW, Orsel K, Nielsen SS, Koets AP, Rutten VPMG, Bannantine JP, et al. Knowledge gaps that hamper prevention and control of Mycobacterium avium subspecies paratuberculosis infection. Transbound. Emerg. Dis. 2018; 65:125–48. 
50.     Alcaldía Municipal de Tuta. Nuestro municipio [Internet]. 2022 [cited 2021 Apr 25]. Available from: http://www.tuta-boyaca.gov.co/municipio/nuestro-municipio
51.     Alcaldía de Tuta. Nuestro Municipio [Internet]. sitio oficial de Tuta. 2013 [cited 2022 Apr 25]. Available from: http://www.tuta-boyaca.gov.co/informacion_general.shtml#economia
52.     ICA. Censo Pecuario Nacional año 2020. Instituto Colombiano Agropecuario. Bogotá; 2020. 
53.     Figueiredo Marques G, Augusto Pompei JC, Martini M. Manual Veterinario de Toma y Envío de Muestras 2017. Panaftosa. 2017;112. 
54.     Fonseca Martinez BA, Leotti VB, Silva G de S e., Nunes LN, Machado G, Corbellini LG. Odds ratio or prevalence ratio? An overview of reported statistical methods and appropriateness of interpretations in cross-sectional studies with dichotomous outcomes in veterinary medicine. Front. Vet. Sci. 2017; 4:193. 
55.     Martin SW, Meek AH, Willebreg P. Veterinary epidemiology: Principles and methods. Zaragoza (España): Acribia S. A; 1997. 384 p. 
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Volume 14, Issue 2 - Serial Number 27
This issue XML file is being prepared.
June 2022
Pages 38-46
  • Receive Date: 22 January 2022
  • Revise Date: 18 May 2022
  • Accept Date: 14 June 2022
  • First Publish Date: 14 June 2022