Evaluation of Antibacterial Activity of cLFchimera and its Synergistic Potential with Vancomycin against Methicillin-Resistant Staphylococcus aureus

Document Type : Research Article

Authors

1 Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

Frequent and unlimited use of antibiotics caused the development of antibiotic resistance by microorganisms. Therefore, there is an argent need to discover novel antibacterial agents or a combination of agents as a safe treatment strategy for various infections. In the present study the synergistic effects of cLFchimera, an antimicrobial peptides, and Vancomycin antibiotic were evaluated using checkerboard method against Methicillin Resistant Staphylococcus aureus (MRSA) bacteria strain. cLFchimera had antimicrobial activity against MRSA and Methicillin Sensitive Staphylococcus aureus (MSSA) (MIC: 256 and 512 µg/mL, respectively). A synergistic effect was observed in the combination of cLFchimera with Vancomycin (FIC: 0.375). The results showed that at FIC concentrations, release cytoplasmic materials from bacterial cells and the number of surviving cells were significantly (P≤0.05) higher and lower, respectively, than when peptides or antibiotic were used alone. SEM electron microscopic analysis at FIC concentration showed severe membrane damage of bacterial cells. In conclusion, the use of cLFchimera and Vancomycin at FIC concentration reduces the consumption of both substances.

Keywords


1. Stapleton PD, Taylor PW. Methicillin resistance in Staphylococcus aureus: mechanisms and modulation. Science Progress. 2002;85(1):57-72.
2. Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. The Journal of Clinical Investigation. 2003;111(9):1265-73.
3. Peacock SJ, Paterson GK. Mechanisms of methicillin resistance in Staphylococcus aureus. Annual Review of Biochemistry. 2015;84: 577-601.
4. Walsh C, Wencewicz T. Antibiotics: challenges, mechanisms, opportunities: John Wiley & Sons; 2016.
5. Zeng D, Debabov D, Hartsell TL, Cano RJ, Adams S, Schuyler JA, et al. Approved glycopeptide antibacterial drugs: mechanism of action and resistance. Cold Spring Harbor Perspectives in Medicine. 2016;6(12):1-16.
6. Wang S, Zeng X, Yang Q, Qiao S. Antimicrobial peptides as potential alternatives to antibiotics in food animal industry. International Journal of Molecular Sciences. 2016;17(5):603-615.
7. Linde A, Ross C, Davis E, Dib L, Blecha F, Melgarejo T. Innate immunity and host defense peptides in veterinary medicine. Journal of Veterinary Internal Medicine. 2008;22(2):247-265.
8. Pirkhezranian Z, Tanhaeian A, Mirzaii M, Sekhavati MH. Expression of Enterocin-P in HEK platform: evaluation of its cytotoxic effects on cancer cell lines and its potency to interact with cell-surface glycosaminoglycan by molecular modeling. International Journal of Peptide Research and Therapeutics. 2019:1-10.
9. Rodríguez-Rojas A, Moreno-Morales J, Mason AJ, Rolff J. Cationic antimicrobial peptides do not change recombination frequency in Escherichia coli. Biology Letters. 2018;14(3):777-782.
10. Tanhaiean A, Azghandi M, Razmyar J, Mohammadi E, Sekhavati MH. Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens. Microbial Pathogenesis. 2018;122:73-85.
11. Tanhaieian A, Sekhavati MH, Ahmadi FS, Mamarabadi M. Heterologous expression of a broad-spectrum chimeric antimicrobial peptide in Lactococcus lactis: Its safety and molecular modeling evaluation. Microbial Pathogenesis. 2018;125:51-69.
12. Tanhaeian A, Ahmadi FS, Sekhavati MH, Mamarabadi M. Expression and purification of the main component contained in camel milk and its antimicrobial activities against bacterial plant pathogens. Probiotics and Antimicrobial Proteins. 2018;10(4):787-93.
13. Tahmoorespur M, Azghandi M, Javadmanesh A, Meshkat Z, Sekhavati MH. A Novel Chimeric Anti-HCV Peptide Derived from Camel Lactoferrin and Molecular Level Insight on Its Interaction with E2. International Journal of Peptide Research and Therapeutics. 2019:1-13.
14. Tanhaeian A, Jaafari MR, Ahmadi FS, Vakili‐Ghartavol R, Sekhavati MH. Secretory expression of a chimeric peptide in Lactococcus lactis: assessment of its cytotoxic activity and a deep view on its interaction with cell-surface glycosaminoglycans by molecular modeling. Probiotics and Antimicrobial Proteins. 2019;11(3):1034-1041.
15. Daneshmand A, Kermanshahi H, Sekhavati MH, Javadmanesh A, Ahmadian M. Antimicrobial peptide, cLF36, affects performance and intestinal morphology, microflora, junctional proteins, and immune cells in broilers challenged with E. coli. Scientific Reports. 2019;9(1):1-9.
16. Fadli M, Saad A, Sayadi S, Chevalier J, Mezrioui N-E, Pagès J-M, et al. Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection–bacteria and their synergistic potential with antibiotics. Phytomedicine. 2012;19(5):464-471.
17. Liu H, Pei H, Han Z, Feng G, Li D. The antimicrobial effects and synergistic antibacterial mechanism of the combination of ε-Polylysine and nisin against Bacillus subtilis. Food Control. 2015;47:444-450.
18. Maisetta G, Mangoni ML, Esin S, Pichierri G, Capria AL, Brancatisano FL, et al. In vitro bactericidal activity of the N-terminal fragment of the frog peptide esculentin-1b (Esc 1–18) in combination with conventional antibiotics against Stenotrophomonas maltophilia. Peptides. 2009;30(9):16-22.
19. Gupta K, Singh S, Van Hoek ML. Short, synthetic cationic peptides have antibacterial activity against Mycobacterium smegmatis by forming pores in membrane and synergizing with antibiotics. Antibiotics. 2015;4(3):358-378.
20. Regmi S, Choi YH, Choi YS, Kim MR, Yoo JC. Antimicrobial peptide isolated from Bacillus amyloliquefaciens K14 revitalizes its use in combinatorial drug therapy. Folia Microbiologica. 2017;62(2):127-138.
21. Nuding S, Frasch T, Schaller M, Stange EF, Zabel LT. Synergistic effects of antimicrobial peptides and antibiotics against Clostridium difficile. Antimicrobial Agents and Chemotherapy. 2014;58(10):5719-5725.
22. Zasloff M. Antimicrobial peptides in health and disease. New England Journal of Medicine. 2002;347(15):1199-1215.
23. Michael N, Stuart B. Molecular mechanisms of antibacterial multidrug resistance.Cells. 2000;128(3): 1037-1050.
24. Brogden KA. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nature Reviews Microbiology. 2005;3(3):238-250.
25. Niyonsaba F, Ushio H, Hara M, Yokoi H, Tominaga M, Takamori K, et al. Antimicrobial peptides human β-defensins and cathelicidin LL-37 induce the secretion of a pruritogenic cytokine IL-31 by human mast cells. The Journal of Immunology. 2010;184(7):3526-3534.
26. Soren O, Brinch KS, Patel D, Liu Y, Liu A, Coates A, et al. Antimicrobial peptide novicidin synergizes with rifampin, ceftriaxone, and ceftazidime against antibiotic-resistant Enterobacteriaceae in vitro. Antimicrobial Agents and Chemotherapy. 2015;59(10):6233-6240.
27. Zhou Y, Peng Y. Synergistic effect of clinically used antibiotics and peptide antibiotics against Gram-positive and Gram-negative bacteria. Experimental and Therapeutic Medicine. 2013;6(4):1000-1004.
28. Shang D, Liu Y, Jiang F, Han X. Synergistic antibacterial activity of Trp-containing antibacterial peptides in combination with antibiotics against multidrug-resistant Staphylococcus epidermidis. Frontiers in Microbiology. 2019;10:27-39.
29. Wu X, Li Z, Li X, Tian Y, Fan Y, Yu C, et al. Synergistic effects of antimicrobial peptide DP7 combined with antibiotics against multidrug-resistant bacteria. Drug Design, Development and Therapy. 2017;11:939-1012.
30. Reyes-Cortes R, Acosta-Smith E, Mondragón-Flores R, Nazmi K, Bolscher JG, Canizalez-Roman A, et al. Antibacterial and cell penetrating effects of LFcin17–30, LFampin265–284, and LF chimera on enteroaggregative Escherichia coli. Biochemistry and Cell Biology. 2017;95(1):76-81.
31. Pirkhezranian Z, Tahmoorespur M, Daura X, Monhemi H, Sekhavati MH. Interaction of camel Lactoferrin derived peptides with DNA: a molecular dynamics study. BMC Genomics. 2020;21(1):60-72.
32. Pirkhezranian Z, Tahmoorespur M, Monhemi H, Sekhavati MH. Computational Peptide Engineering Approach for Selection the Best Engendered Camel Lactoferrin-Derive Peptide with Potency to Interact with DNA. International Journal of Peptide Research and Therapeutics. 2020:1-10.
33. Kapoor G, Saigal S, Elongavan A. Action and resistance mechanisms of antibiotics: A guide for clinicians. Journal of Anaesthesiology, Clinical Pharmacology. 2017;33(3):300-305.
34. van der Kraan MI, van Marle J, Nazmi K, Groenink J, van’t Hof W, Veerman EC, et al. Ultrastructural effects of antimicrobial peptides from bovine lactoferrin on the membranes of Candida albicans and Escherichia coli. Peptides. 2005;26(9):1537-1542.
35. CLSI. Performance Standards for Antimicrobial Susceptibility Testing 28th ed CLSI supplement M100 Wayne, PA: Clinical and Laboratory Standards Institute. 2018.
36. Amin Mir M, Sawhney S, Manmohan SJ. Antimicrobial Activity of Various Extracts of Taraxacum officinale. Journal of Microbial and Biochemical Technology. 2016;8(3):210-215.
37. Habibipour R, Moradi Haghgou L. Study on hydro-alcoholic extract effect of pomegranate peel on Pseudomonas aeruginosa biofilm formation. Scientific Journal of Hamadan University of Medical Sciences. 2015;22(3):195-202.
38. Wendakoon C, Calderon P, Gagnon D. Evaluation of selected medicinal plants extracted in different ethanol concentrations for antibacterial activity against human pathogens. Journal of Medicinally Active Plants. 2012;1(2):60-68.
39. Roshanak S, Shahidi F, Tabatabaei F, Javadmanesh A. Evaluation of Antimicrobial Activity of Buforin I and Nisin and Synergistic Effect of the Combination of them as a Novel Antimicrobial Preservative. The Journal of Food Protection. 2020;43(1):140-153.
40. Mackay M, Milne K, Gould I. Comparison of methods for assessing synergic antibiotic interactions. International Journal of Antimicrobial Agents. 2000;15(2):125-134.
41. Magalhães L, Nitschke M. Antimicrobial activity of rhamnolipids against Listeria monocytogenes and their synergistic interaction with nisin. Food Control. 2013;29(1):138-142.
Volume 14, Issue 1 - Serial Number 26
This issue XML file is being prepared.
April 2022
Pages 1-8
  • Receive Date: 21 September 2021
  • Revise Date: 27 October 2021
  • Accept Date: 30 October 2021
  • First Publish Date: 07 November 2021