Parasitological and pathological findings of coccidiosis in an experimental infection caused by Eimeria ahsata in lambs

Document Type : Research Article

Authors

1 Graduate student of the Veterinary Parasitology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

3 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

    This study was conducted to investigate the pathogenesis process of E. ahsata and its morphological, pathological, and distribution of lesions in the involved tissues during 42 days of infection. Twelve lambs were randomly divided into two groups including the control and the infected groups after confirmation of their health. The animals in the experiment group were orally infected with 1× 105 sporulated oocysts. From 7 days after inoculation (DAI), the feces were sampled and oocysts per gram of feces (OPG) were individually examined for each lamb. . At 7, 14, 21, 28, 35, and 42 DAI, one lamb from each group was necropsied and the abomasum, small and large intestine, mesenteric lymph nodes, spleens, and livers were grossly investigated. From 21 to 42 DAI, mild to severe clinical lesions such as congestion and edema were seen on the mucosal surface of the small intestine associated with white and small foci about 1-2 mm, especially jejunum and ileum. From 7 DAI to the end of the study various stages of the parasite life cycle, infiltration of inflammatory cells, epithelial hyperplasia of villi, and destruction of villi epithelium were seen.  The results showed that E. ahsata was pathogenic in lambs and the macro and microscopic lesions were mostly seen in the jejunum. 

Keywords


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