Hard ticks circulate Anaplasma spp. in South-Khorasan province, Iran

Document Type : Short communication

Authors

1 Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

2 Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

4 Department of Nutrition and Animal Breeding, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

5 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

6 Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran

7 Damavand Agricultural College, Technical and Vocational University, Tehran, Iran

8 North West of Tehran Health Center, Iran University of Medical Sciences, Tehran, Iran

9 Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract

Ticks are vectors for several important zoonoses including different species of Anaplasma. The present study aims to determine the presence of Anaplasma spp. in hard ticks collected from livestock of South-Khorasan province, Iran. A total of 684 livestock were sampled and 269 ticks were collected. Two genera and 6 species of ticks were identified including Rhipicephalus sanguineus, Hyalomma detritium, Hyalomma marginatum, Hyalomma anatolicum, Hyalomma asiaticum, Hyalomma dromedarii and Hyalomma spp. Eleven Hyalomma nymphs and 3 Rhipicephalus nymphs were also identified. 100 Out of 269 ticks were chosen for molecular detection. DNA was extracted followed by PCR technique to detect Anaplasma spp. The presence of Anaplasma spp. was confirmed in 20 out of 100 tested samples (20%). All positive samples collected from Birjand county were Rhipicephalus sanguineus. Results of the present study showed a relatively high infection rate of Anaplasma in hard ticks in South-Khorasan Province.

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1. Hosseini-Vasoukolaei N, Oshaghi MA, Shayan P, Vatandoost H, Babamahmoudi F, Yaghoobi-Ershadi MR, et al. Anaplasma infection in ticks, livestock and human in Ghaemshahr, Mazandaran Province, Iran. J Arthropod Borne Dis. 2014;8(2):204.
2. Noaman V. Identification of hard ticks collected from sheep naturally infected with Anaplasma ovis in Isfahan province, central Iran. Comp Clin Path. 2012;21(3):367–9.
3. Ranjbar R, Anjomruz M, Enayati AA, Khoobdel M, Rafinejad A, Rafinejad J. Anaplasma Infection in Ticks in Southeastern Region of Iran. J Arthropod Borne Dis. 2020;
4. Hurtado OJB, Giraldo-Ríos C. Economic and health impact of the ticks in production animals. In: Ticks and Tick-Borne Pathogens. IntechOpen; 2018.
5. Iran SC of. Country livestock statistics [Internet]. 2017. Available from: https://www.amar.org.ir/Portals/0/News/1396/a-dams96.pdf
6. Wallace MR, Hale BR, Utz GC, Olson PE, Earhart KC, Thornton SA, et al. Endemic infectious diseases of Afghanistan. Clin Infect Dis. 2002;34(Supplement_5):S171–207.
7. Walker AR. Ticks of domestic animals in Africa: a guide to identification of species. Bioscience Reports Edinburgh; 2003.
8. Rar VA, Livanova NN, Panov V V, Kozlova I V, Pukhovskaya NM, Vysochina NP, et al. Prevalence of Anaplasma and Ehrlichia species in Ixodes persulcatus ticks and small mammals from different regions of the Asian part of Russia. Int J Med Microbiol. 2008;298:222–30.
9. Tajedin L, Bakhshi H, Faghihi F, Telmadarraiy Z. High infection of Anaplasma and Ehrlichia spp. among tick species collected from different geographical locations of Iran. Asian Pacific J Trop Dis. 2016;6(10):787–92.
10. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547–9.
11. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10(3):512–26.
12. Jafarbekloo A, Bakhshi H, Faghihi F, Telmadarraiy Z, Khazeni A, Oshaghi MA, et al. Molecular detection of Anaplasma and Ehrlichia infection in ticks in borderline of Iran-Afghanistan. J Biomed Sci Eng. 2014;7(11):919.
13. Bekloo AJ, Bakhshi H, Soufizadeh A, Sedaghat MM, Bekloo RJ, Ramzgouyan MR, et al. Ticks circulate Anaplasma, Ehrlichia, Babesia and Theileria parasites in North of Iran. Vet Parasitol. 2017;248:21–4.
14. Ebrahimzadeh Abkooh E, Pazhoom F, Nabian S, Shayan P. Anaplasma infection in ticks infestating sheep and goat grazed in Savadkooh, Mazandaran. In: The 6 th International Congress of Clinical Laboratory and Clinic. 2014.