A newly discovered interference of the central nitrergic system on oxytocin-induced hypophagia in layer-type chickens

Document Type : Research Article

Authors

Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Abstract

   Various neurochemical pathways are participating in the regulation of food intake in mammals and birds. Both oxytocin (OT) and nitric oxide (NO) are known as hypophagic agents in birds.  This study consisted of 6 experiments and each experiment had 4 groups (ngroup=11, 5-day-old chickens). In all experiments, 3-hour food-deprived (FD3) birds received intracerebroventricular (ICV) injections either control diluent or drug solution. Then the birds had ad libitum access to the food and fresh water and then cumulative food intake (gr) was measured based on the percentage of the body weight (%BW). In experiments 1 to 3, ICV injections of L-arginine (precursor of NO, 200, 400, and 800 nmol), L-NAME (NOS inhibitor, 100, 200, and 400 nmol) and OT (2.5, 5, and 10 µg) were performed respectively. In experiment 4, each group received any ICV injections of L-NAME (100 nmol), OT (10 µg) or a co-injection of L-NAME (100 nmol) and OT (10 µg). In experiment 5, L-arginine (ICV, 200 nmol), OT (10 µg), or L-arginine (200 nmol) and OT (10 µg) were injected to the groups. Experiment 6 was similar to the experiment 5, although the dose of OT was 2.5 µg in all the treatment groups. Results showed that the ICV injection of L-NAME (100 nmol) significantly attenuated hypophagic effect induced by OT (10 µg) (p < 0.05). Findings suggested that NO might mediate the hypophagic effect of OT in FD3 neonatal layer-type chickens. 

Keywords

Main Subjects


Article Title [فارسی]

نقش میانجیگری مسیر نیتررژیک مرکزی بر هیپوفاژی القایی توسط اکسی توسین در جوجه های تخمگذار

Authors [فارسی]

  • مرتضی زنده دل
  • مینا خدادادی
  • حمیرا زندیه
  • کسری مختارپوریانی
  • بهروز رحمانی
  • علی باغبان زاده
گروه علوم پایه/ بخش فیزیولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
Abstract [فارسی]

سیستم های نوروترانسمیتری متنوعی در تنظیم اخذ غذا در پستانداران و پرندگان نقش دارند. اکسی توسین و نیتریک اسید بعنوان عوامل هیپوفاژیک در پرندگان شناخته شده اند. فرضیه ما احتمال یک تداخل بین سیستم نیتررژیک و اکسی توسین در تنظیم اخذ غذا در جوجه های تخمگذار نوزاد بود. در تمامی آزمون ها جوجه های سه ساعت محروم از غذا، محلول کنترل یا داروها را به روش تزریق داخل بطن مغزی دریافت کردند. سپس بصورت آزاد به غذا و آب دسترسی داشتند و اخذ غذای تجمعی (برحسب گرم) در آن ها بر اساس درصدی از وزن بدن اندازه گیری شد. نتایج نشان داد که تزریق داخل بطن مغزی ال-آرژینین (200نانومول) هیپوفاژی القایی توسط اکسی توسین (10 میکروگرم) را تقویت کرد و تزریق همزمان ال-آرژینین (200نانومول) و اکسی توسین ( 2.5 یا 10میکروگرم) اخذ غذا را بطور معنی داری کاهش داد. در نتیجه نیتریک اکساید احتمالاً کاهش اخذ غذای ناشی از اکسی توسین را در جوجه های نوزاد تخمگذار میانجی گری می کند.

Keywords [فارسی]

  • ال-نیم
  • ال-آرژینین
  • اخذ غذا
  • پرنده
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