Detection of mutant infectious bronchitis viruses of GI-23 lineage from commercial chicken flocks in Khorasan Razavi province, Iran in 2019

Document Type : Research Article

Authors

1 Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

2 Veterinary Head Office of Khorasan Razavi, Mashhad, Iran

Abstract

Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is one of the most important respiratory diseases in poultry. The implementation of preventive measures, including vaccination and biosecurity, is necessary for controlling the disease. To maintain biosecurity, it is important to identify the entry route of new viruses into a region and characterizing markers such as unique mutations that make viruses traceable. During a genotyping study for IBV infected commercial chicken flocks in Khorasan Razavi province, 11 viruses from 11 broiler and layer chicken flocks were detected in different cities by PCR. Sequencing of the S1 partial gene followed by phylogenetic analysis showed that eight viruses can be classified in GI-23 lineage (Is-Variant2), two viruses are classified in GI-1 lineage (Mass), and one virus is classified in GI-12 lineage (793B). Although detected viruses of GI-23 lineage are originated from Iran, seven viruses have synonymous (T954C and G1056A) and non-synonymous (C797T) mutations that have not been previously reported. It was found that the new genetic changes in Iranian IBVs of GI-23 lineage occurred in two different regions in Khorasan Razavi. In conclusion, this study indicates that the high prevalence of GI-23 lineage viruses in Iran may enhance the chance of virus mutations and the emergence of new viral strains, so effective vaccination and biosecurity measures are required to control the virus spread.

Graphical Abstract

Detection of mutant infectious bronchitis viruses of GI-23 lineage from commercial chicken flocks in Khorasan Razavi province, Iran in 2019

Keywords


1.    Schalk AF, Hawn MC. An Apparently New Respiratory Disease of Baby Chicks. J Am Vet Med Assoc. 1931;78:413–22.
2.    Jackwood MW, de Wit S. Infectious Bronchitis. In: Swayne DE, Boulianne M, Logue CM, McDougald LR, Nair V, Suarez DL, et al., editors. Diseases of Poultry. 14nd ed. Iowa: Wiley-Blackwell Publishing; 2020. p. 167-88.
3.    Cavanagh D. Coronavirus avian infectious bronchitis virus. Vet Res. 2007;38(2):281-97.
4.    Boursnell M, Brown T, Foulds I, Green P, Tomley F, Binns M. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol. 1987;68(1):57-77.
5.    Denison MR, Graham RL, Donaldson EF, Eckerle LD, Baric RS. Coronaviruses: an RNA proofreading machine regulates replication fidelity and diversity. RNA Biol. 2011;8(2):270-9.
6.    Cavanagh D. Coronavirus IBV glycopolypeptides: size of their polypeptide moieties and nature of their oligosaccharides. J Gen Virol. 1983;64(5):1187-91.
7.    Valastro V, Holmes EC, Britton P, Fusaro A, Jackwood MW, Cattoli G, et al. S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infect Genet Evol. 2016;39:349-64.
8.    Aghakhan S, Abshar N, Fereidouni SRN, Marunesi C, Khodashenas M. Studies on avian viral infections in Iran. Arch Razi Inst. 1994(44/45):1-10.
9.    Hamadan AM, Ghalyanchilangeroudi A, Hashemzadeh M, Hosseini H, Karimi V, Yahyaraeyat R, et al. Genotyping of Avian infectious bronchitis viruses in Iran (2015–2017) reveals domination of IS-1494 like virus. Virus Res. 2017;240:101-6.
10.    Najafi H, Langeroudi AG, Hashemzadeh M, Karimi V, Madadgar O, Ghafouri SA, et al. Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015. Arch Virol. 2016;161(1):53-62.
11.    Homayounimehr A, Pakbin A, Momayyez R, Fatemi SMR. Detection and identification of infectious bronchitis virus by RT-PCR in Iran. Trop Anim Health Prod. 2016;48(5):973-8.
12.    Yousefi Y, Bassami MR, Kalidari GA, Ghahramaniseno MM. Sequence characterization of full-length S1 gene of infectious bronchitis viruses isolated from poultry farms in Khorasan Razavi, Iran. Turk J Vet Anim Sci. 2019;43(2):235-43.
13.    Ganapathy K, Ball C, Forrester A. Genotypes of infectious bronchitis viruses circulating in the Middle East between 2009 and 2014. Virus Res. 2015;210:198-204.
14.    Callison S, Jackwood M, Hilt D. Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates. Avian Dis. 2001:492-9.
15.    Worthington KJ, Currie RJ, Jones RC. A reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006. Avian Pathol. 2008;37(3):247-57.
16.    Cavanagh D, Mawditt K, Britton P, Naylor C. Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions. Avian Pathol. 1999;28(6):593-605.
17.    Hall T, Biosciences I, Carlsbad C. BioEdit: an important software for molecular biology. GERF Bull Biosci. 2011;2(1):60-1.
18.    Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-9.