Biological effects of agricultural bio-materials on some blood and tissue factors in Balb/c mice

Document Type : Research Articles


1 Department of Agronomy, Shahr- e- Qods Branch, Islamic Azad University, Tehran, Iran

2 Department of Agronomy, Karaj Branch, Islamic Azad University, Karaj, Iran

3 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Alborz, Iran.

4 Department of Agronomy, Yadegar-e-Imam Khomeini (RAH) Shahr-e- Rey Branch, Islamic Azad University, Tehran, Iran.


Pseudomonas infections are an important cause of morbidity and mortality and saprophytic fungi are now increasingly being recognized as serious pathogens in immunocompromised patients.To investigate the effect of using bio-materials on mammalian tissues,two experiments were designed;the first one was feeding of Balb/c mice with irrigated lettuce with bio-fungicide (mutant and wild)and bio-fertilizers prepared with Pseudomonas (p) fluorescens, p. putida, p. aeruginosa, and the second was the usage of drinking water containing (Trichoderma (T) spores (mutant and wild) or P.fluorescens, P.putida, P.aeruginosa suspensions). Then, blood factors and inflammation of tissues (liver, kidney, spleen and large intestine) in all mice were analyzed after two months. Blood samples were taken from the mice to examine some of the hematological (RBC, MCV, MCH, MCHC) (data not shown) and biochemical (AST, ALT, ALP) factors, and also observed under a microscope. The study of tumor marker carcinoembryonic antigen (CEA) in all treatments showed that the strains in these bio-fertilizers did not stimulate carcinogenic indices.The results from the other blood factors were normal for all strains (data not shown).Only P.putida showed no adverse effect on the increase in alkaline phosphatase (ALP).The results also showed that the effect of bio-fungicide on mammalian tissues (spleen and large intestine) was normal. But a small number of mild liver necrosis was seen in the treatment groups with wild Trichoderma, and moderate necrosis in the the liver tissue after treatment with mutant Trichoderma isolates.More investigation should be made to determine the impact of these biotic factors on the mammalian tissues before commercialization.


Main Subjects

1. Benitez T, Rincon AM, Limon MC, Codon AC. Biocontrol mechanism of Trichoderma strains.Int. Microbiol. 2004; 7:249–260.
2. Scarpellini M, Franzetti L, Galli A. Development of PCR assay to identify pseudomonas fluorescens and its biotype. FEMS Microbial. Lett. 2004; 236: 257-260.
3. Hsueh PR, et al. Outbreak of Pseudomonas fluorescens bacteremia among oncology patients. J. Clin. Microbiol.1998; 36:2914–2917.
4. Khabbaz RF, et al. Pseudomonas fluorescens bacteremia from blood transfusion. Am. J. Med. 1984; 76:62–68.
5. Scott J, et al. A fatal transfusion reaction associated with blood contaminated with Pseudomonas fluorescens. Vox Sang. 1988; 54:201–204.
6. Taber TE, Hegeman TF, York SM, Kinney RA, Webb DH. Treatment of Pseudomonas infections in peritoneal dialysis patients. Perit. Dial. Int. 1991; 11:213–216.
7. Pier GB, Ramphal R. Pseudomonas aeruginosa, 2004; p. 2587–2615. In Mandell GL, Bennett JE, Dolin R (ed.), Mandell, Douglas, and Bennett’s principles and practice of infectious diseases, 6th ed. Churchill Livingstone, New York, NY.
8. Branski LK, Al-Mousawi A, Rivero H, Jeschke MG, Sanford AP, Herndon DN. Emerging infections in burns. SurgInfect.2009;10(5):389–97.
9. Rossolini GM, Mantengoli E. Treatment and control of severe infections caused by multiresistant Pseudomonas aeruginosa. Clin Microbiol Infect. 2005; 11 Suppl 4:17–32.
10. Yoshino Y, Kitazawa T, Kamimura M, Tatsuno K, and Ota Y, Yotsuyanagi H. Pseudomonas putida bacteremia in adult patients: five case reports and a review of the literature. J Infect Chemother 2011; 17:278-82.
11. Martino R, Martinez C, Pericas R, Salazar R, Sola C, Brunet S, et al. Bacteremia due to glucose non-fermenting gram-negative bacilli in patients with hematological neoplasias and solid tumors. Eur J Clin Microbiol Infect Dis 1996; 15:610-5.
12. Carpenter RJ, Hartzell JD, Forsberg JA, Babel BS, Ganesan A. Pseudomonas putida war wound infection in a US Marine: a case report and review of the literature. J Infect 2008; 56:234-40.
13. Korcova J, Koprnova J, Krcmery V. Bacteraemia due to Pseudomonas putida and other Pseudomonas non-aeruginosa in children. J Infect 2005; 51:81.
14. Blazevic DJ, Koepcke MH, Matsen JM. Incidence and identification of Pseudomonas fluorescens and Pseudomonas putida in the clinical laboratory. Appl Microbiol 1973; 25:107- 10.
15. Woo SL, Ruocco, M, Vinale F, Nigro M, Marra R, Lombardi N, Pascale A, Lanzuise S, Manganiello G, Lorito M. Trichoderma-based products and their widespread use in agriculture. Open Mycol. J. 2014; 8: 71–126.
16. Chouaki T, Lavarde V, Lachaud L, Raccurt CP, Hennequin C. Invasive infections due to Trichoderma species: report of 2 cases, findings of in vitro susceptibility testing, and review of the literature. Clin Infect Dis .2002; 35: 1360-1367.
17. Myoken Y, Sugata T, Fujita Y, Asaoku H, Fujihara M, Mikami Y. Fatal necrotizing stomatitis due to Trichoderma longibrachiatum in neutropenic patient with malignant lymphoma: a case report. Int J Oral Maxillofac Surg. 2002; 31: 688-691.
18. De Miguel D, Gomez P, Gonzalez R, et al. Nonfatal pulmonary Trichoderma viride infection in an adult patient with acute myeloid leukemia: report of one case and review of literature. Diagn Microbiol Infect Dis. 2005; 53: 33-37.
19. Alanio A, Brethon B, Feuilhade de Chauvin M, et al. Invasive pulmonary infection due to Trichoderma longibrachiatum mimicking invasive aspergillosis in a neutropenic patient successfully treated with voriconazole combined with caspofungin. Clin Infect Dis.2008; 46: e116- e118.
20. Kiyama T1, Onda M, Tokunaga A, Nishi K, Mizutani T, Yoshiyuki T, Shimizu Y, Matsukura N, Tanaka N, Asano G. Changes in serum and tissue carcinoembryonic antigen with growth of human gastric cancer xenograft in nude mice. Jpn J Cancer Res. 1990; 81(1):58-62.
21. Almeida AS, Faleiros ACG, Teixeira DNS, Cota UA, Chica, JEL.Reference values for blood- based biochemical parameters in BALB/C and C57BL/6 wild-type mice. Jornal Brasileiro de Patologia e Medicina Laboratorial.2008; (44)6, p. 29-32.
22. Branco ACSC, Diniz MFFM, Almeida RN, Santos HB, Oliveira KM, Ramalho JA, Dantas JG. Biochemical and hematological parameters of wistar rats and Swiss mice in the Professor Thomas George Animal Laboratory. Revista Brasileira de Ciências da Saúde,. 2011; 15(2): 209-214.
23. Al-Muhammadawi KJ. Biochemical and molecular study of exotoxin A from local isolate Pseudomonas aeruginosa.Ph.D. Thesis. College of science. Al-Mustansiriyah University, 2006.
24. Olgerts R. Pavlovskis, Frank A. Voelker, and Arligues H. Shackelford. Pseudomonas aeruginosa Exotoxin in Mice: Histopathology and Serum Enzyme Changes. The Journal of Infectious Diseases. 1976; Vol. 133, No.3.
25. Liu, P. V. Extracellular toxins of Pseudomonas aeruginosa. J. Infect. Dis.130 (Suppl.) 1974 SS94-S99.
26. Rees P. TRICHODEX® (Trichoderma harzianum): Acute Oral Toxicity/Pathogenicity Study in the Rat: (Amended Final Report): Lab Project Number: MAK/103: 91/MAK103/0881: 91/0881. Unpublished study prepared by Life Science Research Ltd. 48 p. Pharmacology & Toxicology. 1991; 16 p.
27. Leuschner P. Acute Toxicity Study of Trichoderma harzianum Strain ICC 012 by Oral Administration to Rats. Project Number: 17792/04. Unpublished study prepared by Laboratory of Pharmacology & Toxicology. 2004; 16 p.
28. Iida A, Sanekata M, Fujita T, Tanaka H, Enoki A, Fuse G, Kanai M, Rudewicz PJ, Tachikawa E. Fungalmetabolites.XVI.Structures of new peptaibols, trichokindins I-VII, from the fungus Trichoderma harzianum. Chem. Pharm. Bull. 1994; 42:1070–1075.
29. Chung JK, Wallace BA. Peptaibols: models for ion channels. Biochem. Soc. Trans. 2001; 29:565–570.