Isolation of broad-host-range bacteriophages against food- and patient-derived Shiga toxin-producing Escherichia coli

Document Type : Original Articles

Authors

1 Ferdowsi University of Mashhad

2 Department of Faculty of Veterinary Medicine

3 Mashhad university of medical sciences

Abstract

This study aimed to isolate bacteriophages specific to Shiga toxin-producing Escherichia coli (E. coli) strains, particularly EHEC O157:H7, in order to develop a collection of phages against different E. coli pathotypes isolated from northeast of Iran. Eighteen samples were screened without any preliminary enrichment and also with small scale enrichment using E. coli 12900, which did not result in the phage recovery. Seven samples were prepared with an extensive enrichment. Of them, 5 samples produced plaques. Eventually, seven phages out of thirteen isolated phages were selected for phage host range investigation. Results of the spotting host range assay demonstrated that 22 pathogenic E. coli strains and isolates (54%) were susceptible to at least one of the phages. Phage Ecol-MHD1 was polyvalent against E. coli and Salmonella isolates. The other phages were specific to E. coli pathotypes. In conclusion, the phages isolated in this study can be suggested as preventive or therapeutic candidates against foodborne E. coli infections in humans.

Keywords


Article Title [فارسی]

جداسازی باکتریوفاژهای وسیع الطیف علیه ایزوله های باکتری اشریشیاکلای تولید کننده شیگاتوکسین جدا شده از مواد غذایی و بیماران

Authors [فارسی]

  • گلشن شاکری 1
  • عبدالله جمشیدی 2
  • کیارش قزوینی 3
1 دانشگاه فردوسی مشهد
3 دانشگاه علوم پزشکی مشهد
Abstract [فارسی]

هدف از این مطالعه جداسازی باکتریوفاژهای اختصاصی STEC و جهت تولید یک مجموعه‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌ی باکتریوفاژ علیه پاتوتایپ های اشریشیاکلای موجود در شمال شرق ایران بود. روشهای پلیت مستقیم و غنی سازی در حجم کم، در گروه A منجر به تشخیص فاژ نشد. در حالیکه غنی سازی کامل در گروه ‌B، منجر به تشخیص فاژ در ۵ نمونه از مجموع ۷ نمونه شد. در ۵۴% باکتری های اشریشیاکلای نسبت به فاژها حساس بودند. فاژ پلی والان Ecol-MHD1، علیه اشریشیاکلای و سالمونلا مؤثر بود. در نتیجه، این فاژها برای کنترل عفونت های ناشی از اشریشیاکلای مناسب هستند.

Keywords [فارسی]

  • ای کولای تولید کننده شیگاتوکسین
  • باکتریوفاژ
  • دامنه میزبانی
  • غنی سازی
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