The anti-planktonic and anti-biofilm formation activity of Iranian pomegranate peel hydro-extract against Staphylococcus aureus

Document Type : Original Articles

Authors

Shahid Bahonar University of Kerman

Abstract

Staphylococcal infections and contaminations have elicited a growing and perennial concern in the medical and food industries. Meanwhile, the manifestation of antibiotic-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA) beside the production of disinfectant-resistant biofilms makes the confrontation with the bacteria more cumbersome and challenging. Pomegranate peel as a waste product of juicing factories is a natural antibacterial agent. The pomegranate peel hydro-extract (PPHE), as a bio-friendly material, was prepared from an Iranian pomegranate cultivar, Rabab, and its phenolic compounds and antioxidant (via DPPH and FRAP assays) and anti-staphylococcal (anti-planktonic and anti-biofilm) properties were assessed. The Rabab PPHE inhibited planktonic cells and biofilm formation of three S. aureus. The Rabab PPHE produced large and obvious staphylococcal inhibition zones in which their diameters were significantly dose-dependent for the milk isolated S. aureus (p < 0.05). Despite the resistance of MRSA (ATCC 33591) to beta-lactam antibiotics, the minimum inhibitory concentration (MIC) of PPHE against its planktonic cells was only 3.75mg mL-1. Furthermore, Rabab PPHE inhibited bacterial biofilms formation in a dose-dependent manner. The MIC of Rabab PPHE against planktonic milk-isolated S. aureus, S. aureus (ATCC 29737), and MRSA prevented 47, 36, and 26% of their biofilm formation, respectively. This addresses the differences between the anti-planktonic and anti-biofilm activity of Rabab PPHE. The anti-planktonic and to a lesser extent the anti-biofilm forming activity of this water-based extract supports the notion of its effectiveness and salubrious application in food and pharmaceutical industries.

Keywords


Article Title [فارسی]

فعالیت عصاره آبی پوست انار ایرانی علیه سلولهای پلانکتونیک و تشکیل بیوفیلم استافیلوکوکوس اورئوس

Authors [فارسی]

  • هادی ابراهیم نژاد
  • مریم عبادی
  • لادن منصوری نژند
Abstract [فارسی]

عفونتهای استافیلوکوکی باعث نگرانی رو به رشد درصنایع پزشکی وغذایی شده اند.ظهور سویه های مقاوم به آنتی بیوتیک مانند استافیلوکوکوس اورئوس مقاوم به متی سیلین(MRSA)در کنارتولید بیوفیلم های مقاوم در برابر ضدعفونی کننده ها، مقابله با این باکتریها را سختترمی کند. پوست انارمحصول ضایعات کارخانه های آبمیوه گیری، یک ماده ضد باکتری طبیعی است. عصاره آبی پوست انار(PPHE)، به عنوان یک ماده دوستدار حیات، از یک رقم انارایرانی،رباب، تهیه شد و ترکیبات فنلی و ویژگیهای آنتی اکسیدانی(توسط آزمونهای DPPH وFRAP) و ضداستافیلوکوکی آن(علیه سلولهای پلانکتونیک و بیوفیلم) ارزیابی شد.عصاره آبی پوست انار رباب سلولهای پلانکتونیک و تشکیل بیوفیلم توسط سه استافیلوکوکوس اورئوس را مهار کرد. عصاره آبی پوست انار رباب هاله های واضح و بزرگ مهار استافیلوکوک را تولید کرد بطوریکه قطرآن دررابطه با استافیلوکوکوس اورئوس جداشده از شیر بطور معنی داری وابسته به دوزبود (p<0/05). با وجود مقاومت استافیلوکوکوس اورئوس مقاوم به متی سیلین (ATCC 33591)در برابرآنتی بیوتیک بتا-لاکتام، حداقل غلظت مهارکننده عصاره علیه سلولهای پلانکتونیک تنها 3/75 میلی گرم درمیلی لیتربود.علاوه برآن،عصاره آبی پوست اناررباب تشکیل بیوفیلم های باکتریایی را بطور وابسته به دوز مهارکرد.حداقل غلظت مهارکننده عصاره آبی پوست اناررباب علیه سلولهای پلانکتونیک استافیلوکوکوس اورئوس جداشده از شیر،استافیلوکوکوس اورئوس(ATCC 29737) واستافیلوکوکوس اورئوس مقاوم به متی سیلین ازتشکیل بیوفیلم های آنها به ترتیب به میزان 47، 36 و 26% جلوگیری کرد.این مساله به تفاوتهای بین فعالیت ضدپلانکتونیک وضدبیوفیلمی عصاره آبی پوست اناررباب اشاره می کند. فعالیت ضدپلانکتونیک وبه میزان کمتری ضدبیوفیلمی این عصاره ی برپایه ی آب، باعث تصور کاربرد موثر آن در صنایع غذایی ودارویی می شود.

Keywords [فارسی]

  • پوست انار
  • رباب
  • استافیلوکوکوس اورئوس
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