Detection and identification of avian adenovirus in broiler chickens suspected of inclusion body hepatitis in Khuzestan, Iran during 2015-2016

Document Type : Research Articles


1 Shahid Chamran University of Ahvaz

2 Shahid Chamran university

3 Shahid Chamran University


Avian adenoviruses (AAV) are known as a very diverse group of pathogens causing a variety of clinical symptoms or being totally asymptomatic in poultry flocks. The aim of this study was the molecular detection of avian adenoviruses in broiler flocks suspected of the IBH and respiratory syndrome in the southwest of Iran. For this intent, the liver and lung samples with macroscopic lesions were collected from 30 different poultry flocks (poultry of slaughterhouse and flock mortalities). Subsequently, DNA was extracted from samples and examined using PCR. The L1 (Loop1) region of the hexon gene was amplified. PCR products were sequenced to reveal the identity of the avian adenoviruses. The data resulted from the nucleo
tide sequencing were analyzed using programs and services provided by National Center for Biotechnology Information (NCBI). The results showed that the pools of liver samples from a 25 days old flock were positive in the PCR test. Based on the sequence data, adenoviruses belonged to the D genotype of avian adenoviruses. In phylogenetic analysis, FADV isolates were closely related to the FADV-11 isolates of Iran, China, Canada and Australia with nucleotide homology up to 99%. This is the first study on molecular detection and analyzing the nucleotide sequence of hexon gene fragment of FADV in broiler farms in Southwest Iran.


Main Subjects

1. P. De Herdt, T. Timmerman, P. Defoort, K. Lycke, R. Jaspers. Fowl adenovirus infections in Belgian broilers: a ten-year survey. Vlaams Diergeneeskundig Tijdschrift. 2013; 82: p. 125-132.
2. Nateghi E., Razmyar J., Bassami M.R. Molecular characterization of avian adenoviruses in Iranian broiler flocks. Iranian Journal of Veterinary Research. 2014; 15(2): p. 164-167.
3. Harrach B, BenkĊ M., Both G.W., Brown M., Davison A.J., Echavarria M., Hess M, Jones M.S., Kajon A., Lehm-kuhl H.D., Mautner V., Mittal S.K., Wadell G. Family Adenoviridae. In King A.M.Q. AMJ,CEB,LEJ, editor. Virus Taxonomy: IXth Report of the International Committee on Taxonomy of Viruses. San Diego: Elsevier Academic Press; 2012. p. 125-141.
4. Adair B.McC., Fitzgerald S.D. Group I Adenovirus infections. In Saif Y.M FAM,GJR,LR,NLK,SDE, editor. Diseases of Poultry. 12th ed. Ames: Blackwell Publishing Professional; 2008. p. 252-266.
5. Hosseini H. , Morshed R. Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran. Iranian Journal of Virology. 2012; 6(4): p. 7-12.
6. Gomis S, Goodhope R, Ojkic D, Willson P. Inclusion body hepatitis as a primary disease in broilers in Saskatchewan. Avian Disease. 2006; 50: p. 550-555.
7. Okuda Y, Ono M, Yazawa S, Shibata I, and Sato S.. Experimental infection of specificpathogen-free chickens with serotype-1 fowl adenovirus isolated from a broiler chicken with gizzard erosions. Avian Disease. 2001; 45: p. 19-25.
8. Toro H, Gonzales C, Cerda L, Hess M, Reyes E, Geisse C. Chicken anemia virus and fowl adenoviruses: association to induce the inclusion body hepatitis/hydropericardium syndrome. Avian Disease. 2000; 44: p. 51-58.
9. Steer PA and Noormohammadi AH. Inclusion Body Hepatitis Outbreaks in Australian Meat Breeder and Broiler Flocks Australia: RIRDC Publication; 2011.
10. M. H. Fowl adenovirus infections in chickens: current status and control approaches.. In Proceedings of the XVII World Veterinary Poultry Congress; 2011; Cancun, Mexico. p. 41-48.
11. Meuleman G, Boschmans M, van den Berg TP, Decaesstecker M. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathology. 2001; 30(6): p. 655-660.
12. Ojkic D, Martin E, Swinton J, Vaillancourt J P, Boulianne M, and Gomis S. Genotyping of Canadian isolates of fowl adenoviruses. Avian Pathology. 2008; 37(1): p. 95-100.
13. P. M. Canadian Poultry Consultants Ltd. [Online].; 2003. Available from: www.canadianpoultry. ca/cms_pdfs/IBH_Final1pdf.
14. Lim TH, Lee HJ, Lee DH, Lee YN, Park JK, Youn HN, et al. Identification and virulence characterization of fowl adenoviruses in Korea. Avian Disease. 2011; 55(4): p. 554-560.
15. SF. L. Hungarian Veterinary Archive. [Online].; 2010. Available from: handle/10832/330.
16. Alvarado IR, P Villegas, J El-Attrache, E Jensen, G Rosales, F Perozo, LB Purvis. Genetic characterization, pathogenicity and protection studies with an avian adenovirus isolate associated with inclusion body hepatitis. Avian Disease. 2007; 51: p. 27-32.
17. Toro H, C Gonzalez, L Cerda, MA Morales, P Dooner, M Salamero. Prevention of inclusion body hepatitis/hydropericardium syndrome in progeny chickens by vaccination of breeders with fowl adenovirus and chicken anemia virus. Avian Disease. 2002; 46: p. 547-554.