A survey on Feline Leukemia virus infection in cats of Ahvaz district, Iran: Seroprevalence and risk factors

Document Type : Research Articles


Shahid Chamran University of Ahvaz


The purpose of the present survey was to determine the seroprevalence rate of FeLV in cats in Ahvaz district, South-West of Iran, as well as, risk factors such as age, gender, breed, life style and clinical findings were evaluated. Blood samples were collected from 60 companion and 124 stray cats and antibody titers were measured against FeLV with ELISA kits. The seroprevalence was obtained 79.89% (95% CI: 74.1-85.68 percent). Chi-square test showed a significant relationship between age groups and infection (p < 0.01). Infection rate in cats with age below 2 years was significantly less than cats between 3-4 years (p < 0.05) and above 4 years old (p < 0.01). Also the seroprevalence was significantly higher in domestic short hair breed than Persian (p < 0.01). The seroprevalence was higher in stray cats than companion, nevertheless, the difference was not significant (p > 0.05). In conclusion, the seroprevalence was very high in cat’s population of Ahvaz district and there was a significant difference between clinical findings and serological results.


Main Subjects

1. Akhtardanesh B, Ziaali N, Sharifi H, Rezaei S. Feline immunodeficiency virus, Feline leukemia virus and Toxoplasma gondii in stray and household cats in Kerman–Iran: Seroprevalence and correlation with clinical and laboratory findings. Research in Veterinary Science. 2010 Oct;89(2):306-10.
2. Alves F, de Souza Rajao D, Del Puerto HL, Braz GF, Leite RC, Mazur C, et al. Occurrence of Feline immunodeficiency virus and Feline leukemia virus infection in cats. American Journal of Animal Veterinary Science. 2011;6(3):125-9.
3. Bandecchi P, Matteucci D, Baldinotti F, Guidi G, Abramo F, Tozzini F, et al. Prevalence of Feline immunodeficiency virus and other retroviral infections in sick cats in Italy. Veterinary Immunology and Immunopathology. 1992 Mar;31(3-4):337-45.
4. Beatty JA, Tasker S, Jarrett O, Lam A, Gibson S, Noe-Nordberg A, et al. Markers of Feline leukaemia virus infection or exposure in cats from a region of low seroprevalence. Journal of Feline Medicine and Surgery. 2011 Dec;13(12):92733.
5. Cattori V, Tandon R, Riond B, Pepin AC, Lutz H, Hofmann-Lehmann R. The kinetics of Feline leukaemia virus shedding in experimentally infected cats are associated with infection outcome. Veterinary Microbiology. 2009 Jan 13;133(3):292-6.
6. Feldman BF, Zinkl JG, Jain NC, Schalm OW. Reference values. In: Schalm OW, editor. Schalm’s Veterinary Hematology, 6st ed. Blackwell Publishing; 2006.
7. Goldkamp CE, Levy JK, Edinboro CH, Lachtara JL. Seroprevalences of Feline leukemia virus and Feline immunodeficiency virus in cats with abscesses or bite wounds and rate of veterinarian compliance with current guidelines for retrovirus testing. Journal of the American Veterinary Medical Association. 2008 Apr;232(8):1152-8.
8. Hartmann K. Feline Leukemia Virus Infection. In: Greene, CE, editor. Infectious Disease of the Dog and Cat. Vol.1, 4rd ed. Elsevier Health Sciences, Saunders Company; 2012.
9. Hitt ME, Spangler L, McCarville C. Prevalence of Feline immunodeficiency virus in submissions of feline serum to a diagnostic laboratory in Atlantic Canada. The Canadian Veterinary Journal. 1992 Nov;33(11):723-6.
10. Hofmann-Lehmann R, Huder JB, Gruber S, Boretti F, Sigrist B, Lutz H. Feline leukaemia provirus load during the course
of experimental infection and in naturally infected cats. Journal of General Virology. 2001 Jul;82(7):1589-96.
11. Jamshidi S, Saedi A, Bokaie S. Seroepidemiological study of Feline leukemia virus in stray and domestic cats of Tehran. Journal of Veterinary Research. 2008;63:317–9.
12. Jarret O, Hosie MJ. Feline leukemia virus infection. In: Chandler EA, Gaskell CJ, Gaskell RM, editors. Feline Medicine and Therapeutics. 3rd ed. Oxford: Blackwell; 2004.
13. Katrin, H. Feline Leukemia Virus Infection. In: Greene CE, editor. Infectious diseases of the dog and cat. Saunders; 2006.
14. Lee IT, Levy JK, Gorman SP, Crawford PC, Slater MR. Prevalence of Feline leukemia virus infection and serum antibodies against Feline immunodeficiency virus in unowned free-roaming cats. Journal of the American Veterinary Medical Association. 2002 Mar;220(5):620-2.
15. Levy JK, Crawford PC. Feline leukemia virus. In: Ettinger SJ, Feldman EC, editors. Textbook of veterinary internal medicine. Vol.1, 7th ed., St. Louis, Elsevier Saunders; 2010.
16. Levy J, Crawford C, Hartmann K, Hofmann-Lehmann R, Little S, Sundahl E, et al. 2008 American Association of Feline Practitioners’ feline retrovirus management guidelines. Journal of Feline Medicine and Surgery. 2008 Jun;10(3):300-16.
17. Levy JK, Scott HM, Lachtara JL, Crawford PC. Seroprevalence of Feline leukemia virus and Feline immunodeficiency virus infection among cats in North America and risk factors for seropositivity. Journal of the American Veterinary Medical Association. 2006 Feb;228(3):371-6.
18. Little S, Sears W, Lachtara J, Bienzle D. Seroprevalence of Feline leukemia virus and Feline immunodeficiency virus infection among cats in Canada. Canadian Veterinary Journal. 2009 Jun; 50(6):644-8.
19. Malik R, Kendall K, Cridland J, Coulston S, Stuart AJ, Snow D, et al. Prevalences of Feline leukaemia virus and feline immunodeficiency virus infections in cats in Sydney. Australian Veterinary Journal. 1997 May;75(5):323-7.
20. Maruyama S, Kabeya H, Nakao R, Tanaka S, Sakai T, Xuan X, et al. Seroprevalence of Bartonella henselae, Toxoplasma gondii, FIV and FeLV infections in domestic cats in Japan. Microbiology Immunology. 2003;47(2):147-53.
21. Muirden A. Prevalence of Feline leukaemia virus and antibodies to Feline immunodeficiency virus and feline coronavirus in stray cats sent to an RSPCA hospital. Veterinary Research. 2002 May;150(20):621-5.
22. Najafi H, Madadgar O, Jamshidi S, Ghalyanchi Langeroudi A, Darzi Lemraski M. Molecular and clinical study on prevalence of Feline herpesvirus type 1 and calicivirus in correlation with Feline leukemia and immunodeficiency viruses. Veterinary Research Forum. 2014 Autumn;5(4):255-61.
23. Nakamura K, Miyazawa T, Ikeda Y, Sato E, Nishimura Y, Nguyen NT, et al. Contrastive prevalence of feline retrovirus infections between northern and southern Vietnam. J Vet Med Sci. 2001 Aug;63(8):921-3.
24. O’Connor TP Jr, Tonelli QJ, Scarlett JM. Report of the National FeLV/FIV Awareness Project. Journal of Veterinary Medical Science. 1991 Nov;199(10):1348-53.
25. Shahrani F, Doosti A, Arshi A. Molecular study for detection of Feline Leukemia Virus (FeLV) in Iranian cats. African Journal of Microbiology Research. 2011 Aug;5(15):2103-6.
26. Sherding, RG. Feline Leukemia Virus. In: Brichard SJ, Sherding RG, editors. WB Saunders Manual of Small Animal Practice. 3rd ed. Saunders Company; 2006.
27. Sukhumavasi W, Bellosa ML, Lucio-Forster A, Liotta JL, Lee AC, Pornmingmas P, et al. Serological survey of Toxoplasma gondii, Dirofilaria immitis, Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) infections in pet cats in Bangkok and vicinities, Thailand. Veterinary parasitology. 2012 Aug;188(1-2):25-30.
28. Sukura A, Salminen T, Lindberg LA. A survey of FIV antibodies and FeLV antigens in free-roaming cats in the capital area of Finland. Acta Veterinary Scandinavica. 1992;33(1):9-14.
29. Torkan S, Momtaz H, Jafarian Dehkordi M, Khamesipour F. Molecular study of the prevalence of Feline Leukemia Virus (FeLV) in Iranian domestic cats from blood samples by reverse transcription polymerase chain reaction (RT-PCR) in Iran. Int. International Journal of Plant, Animal and Environmental Sciences. 2014;4(2):728-33.
30. Yilmaz H, Ilgaz A, Harbour DA. Prevalence of FIV and FeLV infections in cats in Istanbul. Journal of Feline Medicine and Surgery. 2000 Mar;2(1):69-70.
31. Yuksek N, Kaya A, Altug N, Ozkan C, Agaoglu ZT. Prevalence of Feline retrovirus infections in van cats. Bulletin of Veterinary Institution Pulawy. 2005;49:375-7.