Morphologic and morphometric variations of the adult and the eggs of frequent Fasciola species from domestic ruminants of North West of Iran

Document Type : Research Articles


1 Nazlu campus, Urmia

2 PhD student of Veterinary Parasitolo Shahid Chamran University OF Ahvaz


Worldwide including Iran, Fasciola species are the causes of human and animal fasciolosis which have comparatively identical morphologies. The present study is aimed to identify different Fasciola forms by using morphologic and morphometric variations from domestic ruminants of northwestern Iran. A total of 130, 67 and 140 livers of cattle, water buffaloes and sheep respectively were collected from Urmia slaughterhouse. The adult helminths were removed and stained using Acetocarmine staining. The Fasciola eggs were directly extracted from the uterus of adult helminths. The overall frequency of infection was 28.19%. The highest infection rate was found in water buffalos (34%). The highest number of helminth per each animal was recorded for cattle (9.23%). The predominant infecting fasciolid in the examined ruminants was F. gigantica (51.89%) from water buffaloes origin. There was a significant difference among the width (W), the length (L), and the distance between ventral sucker to the posterior end of the body (DBVE) of all Fasciola forms from the examined animals. The L of different Fasciola forms had a significant difference for F. gigantica and intermediate form of Fasciola from cattle and water buffaloes origins. The W and the proportion of the body length to the width (SI) of eggs from all Fasciola forms had no significant difference. The Ls of eggs of F. hepatica and F. gigantica were significantly different. The results of this study elucidated three forms of Fasciola co-existing in the ruminants of the region. Additionally, the morphology and morphometry of adult and eggs of Fasciola species within a range of hosts may be taxonomically informative and one of the character sets for discrimination of fasciolid forms.


Main Subjects

Ali, M.A.H. (1993) Studies on some tissue parasites of some animals used for human consumption. M.Sc. Thesis, Faculty of Medicine, Assiut University, Egypt.
Agatsuma, T., Terasaki, K., Yang, L., Blair, D. (1994) Genetic variation in triploids of Japanese Fasciola species and relationships with other species in the genus. Journal of Helmintology 68, 181186.
Agatsuma, T., Arakawa, Y., Iwagami, M., Honzako, Y., Cahyaningsih, U., Kang, S.Y., Hong, S.J. (2000) Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica. Parasitology International 49(3), 231-238.
Akahane, H., Harada, Y. and Oshima, T. (1970) Patterns of the variation of the common liver fluke (Fasciola sp.) in Japan. III. Comparative studies on the external form, size of egg and number of eggs in uterus of the fluke in cattle, goat and rabbit. Kisechugaku Zasshi 19, 619– 627.
Ashrafi, K., Valero, M.A., Panova, M., Periago, M.V., Massoud, J., Mas-Coma S. (2006) Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantic and intermediate forms from the endemic region of Gilan, Iran. Parasitology International 55, 249–260.
Bargues, M.D., Fuentes, M.V., Mansoorian, A.B., Moghaddam, A.S., Ashrafi, K., Savioli, L.Y. (2002) Determinacion especifica de los parasitos implicados en la Fascioliasis humana Y animal en la provincial de Gilan, Iran, mediante secuenciacion de and ribosomal nuclear. III Congreso Semiti - Siciedad Epanola de Medicina Tropical Y Salud international, Madrid, Spain.
Cheng, T.C., Bogitsh, J.B. (1998) Fasciola hepatica. Human Parasitology. 2nd ed., Sandiego, Academic Press, pp. 208- 212.
Dalton, JP. (1999) Fasciolosis. 1th ed., CAB International Publishing.
Eslami, A. (2008) Family Fasciolidae. In: Veterinary Helminthology. Tehran University Press, pp. 45-86.
Esteban, J.G., Bargues, M.D., Mas-Coma, S. (1998) Geographical distribution, diagnosis and treatment of human fascioliasis: a review. The Revista Brasileira de Parasitologia Veterinaria 58(1), 13-42.
Farag, H.F., Barakat, R.M., Ragab, M., Omar, E. (1979) A focus of human fascioliasis in the Nile Delta, Egypt. The American Journal of Tropical Medicine and Hygiene 82,188–190.
Ghobadi, K., Yakhchali, M. (2003) Survey of helminthes infection rate of liver and economical losses in Urmia slaughterhouse, Iran. Journal of Faculty of Veterinary Medicine, Tehran University 58(1), 19-20.(in Persian with English abstract)
Imani-Baran, A., Yakhchali, M., Malekzadeh Viayeh, R., Paktarmani, R. (2012) Molecular study for detecting the prevalence of Fasciola gigantica in field-collected snails of Radix gedrosiana (Pulmonata: Lymnaeidae) in northwestern Iran. Veterinary Parasitology 189(2-4), 374-377.
Karimi, M. (2008) Genetic diagnosis of Fasciola species base on 18S rDNA sequences. Journal of biology Science 8(7), 1166-1173.
Kendall, S.B., McCullough. F.S. (1951) The emergence of cercariae of Fasciola hepatica from the snail Lymnaea truncatula. Journal of Helminthology 25, 77–92.
Kimura, S., Shimizu, A., Kawano J. (1984) Morphological observation on liver fluke detected from naturally infected Carabaos in the Philippines. Scientific report of the Faculty of Agriculture, Kobe University Japan, pp. 353-357.
Malek, AM. (1981) Snail-transmited parasitic diseases. CRC Press, Florida, USA. Mayne, M. (2000) Mayer's Pathology of Infectious Diseases. Hepatic trematodes. Armed Forces Institute of Pathology American Registry of Pathology, Washington, DC, USA.
Mas-Coma, S., Bargues, MD. (1997) Human liver flukes: a review. Research and Reviews in Parasitology 57, 145–218.
Mas-Coma, S. (2003) Fascioliasis, the vectorborne parasitic disease with the widest latitudinal, longitudinal and altitudinal distribution known. Proceedings of the International Symposium: Asian unique strategy against Asian parasitic diseases for Asian people by Asian parasitologists (AAA) and the 2nd Congress of the Federation of Asian Parasitologists (FAP), Ministry of Education, Culture, Sports, Science and Technology, Chiba, Japan, pp. 20–22.
Moghaddam, A.S., Massoud, J., Mahmoodi, M., Mahvi, A.H., Periago, M.V., Artigas, P., Fuentes, M.V., Bargues, M.D., Mas-Coma, S. (2004) Human and animal Fascioliasis in Mazandaran province northern Iran. Parasitology Research 94(1), 61-69.
Oshima, T., Akahane, H., Koyama, H., Shimazu, T., Harada, Y. (1968) Patterns of the variation of the common liver fluke (Fasciola sp.) in Japan II. A comparative study of the flukes from cattle and goat. Kisechugaku Zasshi 17, 534–539.
Massoud, J. (1974) Observation on Lymnaea gedrosiana, the intermediate host of Ornithobilharziaturkestanicum in Khuzestan, Iran. Journal of Helminthology 48, 133-138.
Paniker, C.K.J. (1997) Text book of medical parasitology. 4th ed., pp. 129-130.
Parija, SC. (1996) Text book of medical parasitology. 4th ed., pp. 249-253.25. Rim, H.J., Farag, H.F., Sornmani, S., Cross, J.H. (1994) Food-borne trematodes: ignored or emerging. Parasitology Today 10, 207–209.
Sahba, G.H., Arfaa, F., Farahmandian, I., Jalali, H. (1972) Animal fascioliasis in Khuzestan, southwestern Iran. Journal of Parasitology 58, 712-716.
Salahi-Moghaddam, A. (2004) Study of human Fascioliasis and its intermediate host in Mazandaran province. Dissertation, Tehran. Tehran University of Medical Sciences. (in persian with English abstract]
Scmidt, G.D., Roberts, L.S. (2000) Foundation
of parasitology. 6th ed., pp.256-262.
Sinitsin, D.F. (1993) Studien ueber die phylogenie der trematoden. VI. The life history of some American liver flukes. Zeitschrift fuer Parasitenkunde 6, 170– 191.
Soulsby, E.J.L. (1982) Helminths, arthropods and protozoa of domesticated animals. 7th ed., Bailliere Tindall and Company, London, UK, pp. 800-809.
Srimuzipo, P., Komalamisra, C., Choochote, W., Jitpakdi, A., Vanichthanakorn, P., Keha, P., Riyong, D., Sukontasan, K., Komalamisra, N., Sukontasan, K., Tippawangkosol, P. (2000) Comparative morphometry, morphology of egg and adult surface topography under light and scanning electron microscopies, and metaphase karyotype among three sizeraces of Fasciola gigantica in Thailand. The Southeast Asian journal of tropical medicine and public health 31, 366–373.
Terasaki, K., Akahane, H., Habe, S. (1982) The geographical distribution of common liver fluke (The genus Fasciola) with normal and abnormal spermatogenesis. Japanese Journal of Veterinary Science 44, 223-231.
Valero, M.A., Darce, N.A., Panova, M., MasComa, S. (2001) Relationships between host species and morphometric patterns in Fasciola hepatica adults and eggs from the Northern Bolivian Altiplano hyperendemicregion. Veterinary Parasitology 102, 85-100.
Watanabe, S. (1962) Fascioliasis of ruminants in Japan. Bulletin de la Office International Epizooties 58, 313–322.
Yakhchali, M., Ghobadi, K. (2005) A survey on helminths infection of liver and economic loss in slaughtered sheep of Urmia slaughterhouse, Iran. Iranian Veterinary Journal 11, 60-65. (in Persian with English abstract)
Yakhchali, M., Malekzadeh-Viayeh, ImaniBar, A., Mardani, K. (2015) Morphological and molecular discrimination of Fasciola species isolated from domestic ruminants of Urmia city, Iran. Iranian Journal of Parasitology 10(1), 46–55.