Relationships between trace elements, oxidative stress and subclinical ketosis during transition period in dairy cows

Document Type : Research Articles


1 Ferdowsi University of Mashhad, Mashhad, Iran

2 Ferdowsi University of Mas


The possible relationships between trace elements, oxidative stress and subclinical ketosis during the transition period were evaluated in dairy cows. Blood samples were obtained by jugular venipuncture at four times of the transition period: 3 weeks and 1 week before and 1 week and 3 weeks after calving. The highest concentrations of malondialdehyde (MDA) and betahydroxybutyrate (BHB) were observed on the week 1 after calving. In contrast, the serum values of copper, zinc, albumin, uric acid and TIBC decreased after calving. After calving, zinc concentration showed a significant negative correlation with MDA concentration (p


Adela, P., Zinveliu, D., Pop, R.A., Andrei, S. and Kiss, E. (2006) Antioxidant status in dairy cows during lactation. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca 63, 130-135.
Akhtar, M.S., Farooq, A.A. and Mushtaq, M. (2009) Serum trace minerals variation during pre and post-partum period in nili-ravi buffaloes. The Journal of Animal and Plant Sciences 19, 182-184.
Al-Qudah, K. (2011) Oxidant and antioxidant profile of hyperketonemic ewes affected by pregnancy toxemia. Veterinary Clinical Pathology 40, 60-65.
Azab, M.E. and Abdel-Maksoud, H.A. (1999) Changes in some hematological and biochemical parameters during prepartum and postpartum periods in female Baladi goats. Small Ruminant Research, 34, 77-85(9).
Bonnefont Rousselot, D., Bastard, J.P., Jaudon, M.C. and Delattre, J. (2000) Consequences of the diabetic status on the oxidant/antioxidant balance. Diabetes and Metabolism 26, 163–176.
Bell, A.W. (1995) Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. Journal of Animal Science 73, 2804– 2819.
Bernabucci, U., Ronchi, B., Lacetera, N. and Nardone, A. (2002) Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. Journal of Dairy Science 85, 2173–2179.
Bernabucci, U., Ronchi, B., Lacetera, N. and Nardone, A. (2005) Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science 88, 2017–2026.
Castillo, C., Hernandez, J., Bravo, A., LopezAlonso, M., Pereira, V. and Benedito, J.L. (2005) Oxidative status during late pregnancy and early lactation in dairy cows. The Veterinary Journal 169, 286– 292.
Ceylan, A., Sern, L., Aksit, H. and Seyrek, K. (2008) Concentrations of some elements in dairy cows with reproductive disorders. The Bulletin of the Veterinary Institute in Pulawy 52, 109–112.
Duffield, T. (2000) Subclinical ketosis in lactating dairy cattle. Veterinary Clinics of North America: Food Animal Practice 16, 231-253.
Duzguner, V. and Kaya, S. (2007) Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits. Free Radical Biology and Medicine 42, 1481– 1486.
Evans, P. and Halliwell, B. (2001) Micronutrients: oxidant/antioxidant status. British Journal of Nutrition 85, 57–74.
Faure, P., Benhamou, P.Y., Perard, A., Halimi, S. and Roussel, A.M. (1995) Lipid peroxidation in insulin-dependent diabetic patients with early retina degenerative lesions: effects of an oral zinc supplementation. European Journal of Clinical Nutrition 49, 282–288.
Gehrke, M. and Markiewicz, H. (2009) Culling and fertility of cows with energy metabolic disturbances in the early postparturient period. The Bulletin of the Veterinary Institute in Pulawy 53, 375-381.
Goff, J.P. (2006) Macromineral physiology and application to the feeding of the dairy cow for prevention of milk fever and other periparturient mineral disorders. Animal Feed Science and Technology 126, 237–257.
Grummer, R.R., Mashek, D.G. and Hayirli, A. (2004) Dry matter intake and energy balance in the transition period. Veterinary Clinics of North America: Food Animal Practice 20, 447–470.
Jain, S.K., McVie, R. and Bocchini, J.A. (2006) Hyperketonemia (ketosis), oxidative stress and type 1 diabetes. Pathophysiology 13, 163–170.
Kankofer, M., Albera, E., Feldman, M., Gundling, N. and Hoedemaker, M. (2010) Comparison of antioxidative/oxidative profiles in blood plasma of cows with and without retained fetal placental membranes. Theriogenology 74, 1385-1395.
Spears, J.W. and Weiss W.P. (2008) Role of antioxidants and trace elements in health and immunity of transition dairy cows. The Veterinary Journal 176, 70–76.
Lykkesfeldt, J. and Svendsen, O. (2007) Oxidants and antioxidants in disease: oxidative stress in farm animals. The Veterinary Journal 173, 502–511.
McCarthy, S.D., Waters. S.M., Kenny. D.A., Diskin, M.G., Fitzpatrick, R., Patton, J., Wathes, D.C. and Morris, D.G. (2010) Negative energy balance and hepatic gene expression patterns in high-yielding dairy cows during the early postpartum period: a global approach. Physiological Genomics. 42, 188-199.
Meglia, G., Holtenius K, Petersson L, Ohagen P. and Waller KP. (2004) Prediction of vitamin A, vitamin E, selenium and zinc status of periparturient dairy cows using blood sampling during the mid dry period. Acta Veterinaria Scandinavica 45, 119-128.
Miller, J.K., Brzezinska-Slebodzinska, E. and Madsen, F.C. (1993) Oxidative stress, antioxidants, and animal function. Journal of Dairy Science 76, 2812–2823.
Moran, L.K., Gutteridge, J. and Quinlan, G.J. (2001) Thiols in cellular redox signalling and control. Current Medicinal Chemistry 8, 763.
Mudron, P. and Konvicna, J. (2006) Thiobarbituric acid reactive substances and plasma antioxidative capacity in dairy cows at different lactation stages. Deutsch Tierarztl Wochenschr 113, 189-191.
Nordberg, J. and Arner, E.S.J. (2001) Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radical Biology and Medicine 31, 1287– 1312.
Pedernera, M., Celi, P., Garcia, S.C., Salvin, H.E., Barchia, I. and Fulkerson, W.J. (2010) Effect of diet, energy balance and milk production on oxidative stress in early-lactating dairy cows grazing pasture. The Veterinary Journal 186, 352-357.
Placer, Z.A., Cushman, L. and Johnson, B. (1966) Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical system. Analytical Biochemistry 16, 359–364.
Quiroz-Rocha, G.F., LeBlanc, S., Duffield, T., Wood, D., Leslie, K.E. and Jacobs, R.M. (2009a) Evaluation of prepartum serum cholesterol and fatty acids concentrations as predictors of postpartum retention of the placenta in dairy cows. Journal of the American Veterinary Medical Association 234, 790–793.
Quiroz-Rocha, G.F., LeBlanc, S., Duffield, T., Wood, D., Leslie, K.E. and Jacobs, R.M. (2009b) Reference limits for biochemical and hematological analytes of dairy cows one week before and one week after parturition. The Canadian Veterinary Journal 50, 383–388.
Sahoo, S.S., Parta, R.C., Behera, P.C. and Swarup, D. (2009) Oxidative stress indices in the erythrocytes from lactating cows after treatment for subclinical ketosis with antioxidant incorporated in the therapeutic regime. Veterinary Research Communication 33, 281–290.
Sharma, N., Singh, N.K., Singh, O.P., Pandey, V. and Verma, P.K. (2011) Oxidative stress and antioxidant status during transition period in dairy cows. AsianAustralasian Journal of Animal Science 24, 479 - 484.
Shen, X.P., Zou, S.B., Wu, H.J. and Zhang, Y.
(2009) The relationship between serum level of leptin and oxidative stress in patients with hyperglycemia crisis. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 21, 353–653.
(Article in Chinese) Sordillo, L.M. (2005) Factors affecting mammary gland immunity and mastitis susceptibility. Livestock Production Science 98, 89–99.
Sordillo, L.M. and Aitken, S.L. (2009) Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology and Immunopathology 128, 104–109.
Soriani, M., Pietraforte, D. and Minetti, M. (1994) Antioxidant potential of anaerobic human plasma: role of serum albumin and thiols as scavengers of carbon radicals. Archives of Biochemistry and Biophysics 312, 180– 188.
Turk, R., Juretic, D., Geres, D., Svetina, A., Turk, N., Rekic, B. and Simeon-Rudolf, V. (2004) Serum paraoxonase activity and lipid parameters in the early postpartum period of dairy cows. Research in Veterinary Science 76, 57– 61.
Turk, R., Juretic, D., Geres, D., Svetina, A., Turk, N. and Flegar-Mestric, Z. (2008) Influence of oxidative stress and metabolic adaptation on PON1 activity and MDA level in transition dairy cows. Animal Reproduction Science 108, 98 – 106.
Turk, R., Samardžija, M. and Bačić, G. (2011) Oxidative stress and reproductive disorders in dairy cows. In: Marek, E. R., Dairy cows: nutrition, fertility and milk production. Nova Science Publishers, New York, USA, pp, 57-98.
West, I. C. (2000) Radicals and oxidative stress in diabetes. Diabetic Medicine 17, 171–180. Wilde, D. (2006) Influence of macro and micro minerals in the periparturient period on fertility in dairy cattle. Animal Reproduction Science 96, 240–249.
Youssef, M., El-Khodery, S.A., El-deeb, W.M. and Abou El-Amaiem, W.E. (2010) Ketosis in buffalo (Bubalus bubalis): clinical findings and the associated oxidative stress level. Tropical Animal Health and Production 42, 1771-1777.
Zhang, Z,G., Li, X.B., Gao, L., Li, Y.F., Liu, G.W., Wang, H.B. and Wang, Z. (2011) Serum antioxidant capacity of dairy cows with subclinical ketosis. Veterinary Record 168, 22.