Chromosomal analysis of two buffalo breeds of Mazani and Azeri from Iran

Document Type : Research Articles


1 University of Guilan

2 Agriculture Biotechnology Research Institute


In the present study karyotype of Mazani river buffalo was studied in comparison with those of Azeri buffalo populations from Iran. Blood samples were taken from ten (5 males and 5 females) Mazani buffaloes and thirty (15 males and 15 females) Azeri buffaloes. The Mazani buffaloes belong to Mazendaran province and Azeri buffaloes belong to west and east Azerbaijan and Ardebil provinces. Blood lymphocytes cultured at 37ºC for 72 hours in the presence of phytohemagglutinin and the metaphase spreads were performed on microscopic slide. Giemsa was used to stain chromosomes. The Mazani and Azeri Buffalo exhibited the same karyotype with diploid number of 2n = 50. The fundamental numbers (NF) were 60 in male and female. The types of chromosome were 6 submetacentric, 4 metacentric and 40 telocentric which the X chromosome is the largest telocentric and the Y chromosome is one of the smallest telocentric chromosomes. The relative length of chromosomes ranged between 2.17% to 7.2% in Mazani buffalo, and also 2.21% to 6.55% in Azeri buffalo. No obvious abnormality was found among chromosomes. Therefore, based on the identified karyotype both Mazani and Azeri buffaloes are riverine.


Ahmad, I., Javed, K., and Sattar, A. (2004)Screening of breeding bulls of different breeds through karyotyping. Pakistan Veterinary Journal 24,190-192.
Ali, A., Abdullah, M., Javed, K., Babar, M E., Mustafa, H., Ahmad, N. and Akhtar, M. (2012). Cytogenetic and Genome Studies in Pakistani Buffalo (Bubalusbubalis) - A Review. The Journal of Animal and Plant Sciences 22, 225-227.
Balakrishnan, C.R. and Yadav, B.R. (1984). Normal and abnormal chromosomes in the Indian River buffaloes. Buffalo Bulletin 3, 13-17.
Bidhar, G.C.,Pattnaik, G.R., Rao, P.K. and Patro, B.N. (1986). Chromosome number and morphology of paralakhemundi buffaloes in Orissa. Buffalo Bulletin 5(3), 54-56.
Cribiu, E.P. and Obeidah, A. (1978). The C-banding pattern of Egyptian water buffalo (Bubalusbubalis). Annales de genetiqueet de selectionanimale 10(2), 271-274.
De Hondt, H.A. and Ghanam, S.A. (1971). Cytogenetic studies of the Egyptian water buffalo (Bubalusbubalis). Buffalo Journal 1, 33-40.
Gersen, S.L. and Keagle, M.B., (2005). The principle of Clinical Cytogenetics. 2nd edition. New Jersey, Human press; P.63-79.
Guerra, M.D.S. (1986). Short communication reviewing the chromosome nomenclature of Leavanet al. Brazilian Journal of genetics 4, 741-743.
Gupta, P., Chaudhri, S.P. (1978). Robertsonian changes in the chromosomes of Indian Murrah buffalo, Bubalusbubalis. The Nucleus 21, 90-97.
Halnan, C.R.E., (1976). A cytogenetic survey of 1101 Australian cattle of 25 different breeds. Annales de genetiqueet de selectionanimale 8 (2), 131-139.
Harisah, M., Azmi, T.I., Hilmi, M., Vidyadaran, M.K., Bongso, T.A., Nav., Z.M., Momongan, V. and Baasrur, P.K. (1989). Identification of crossbred buffalo genotypes and their chromosome segregation patterns. Genome 32(6), 999-1002.
Hasanzadeh, S. and Monazzah, S. (2011). Gross morphology, histomorphology and histomorphometry of the jejunum in the adult river buffalo. Iranian Journal of Veterinary Research 12, 99-106.
Hasanzadeh, S. and Orojee, S. (2003). Gross, morphology, histology and histomorphometry of the ileum in river buffalo. Buffalo Journal 19, 273-282.
Iannuzzi, L. (1994). Standard karyotype of the river buffalo (Bubalusbubalis L., 2n=50) report of the committee for the standardization of banded karyotypes of the river buffalo. Cytogenetics and Cell Genetics 67, 102-113.
Joshi, S.K. and Govindaiah, M.G. (1997).Karyological studies in South Kanara buffaloes of Karnataka. Indian Veterinary Journals 74, 1037 – 1039.
Kenthao, A., Tanomtong, A., Supanuam, P., Pinyoteppratan, C., Muangprom, P., Buranarom, P. and Sanoamuang, L. (2012). Standardize karyotype and idiogram of Mehsani Buffaloes, Bubalusbubalis by conventional staining, GTG-Banding, CBG-Banding and AG-NOR Banding techniques. Buffalo Bulletin 31, 24-39.
Khavary, H. (1978). [Le caryotype normal du buffle d'Iran (Bubalusbubalis)]. Bulletin de la Societe des sciences 80, 203-205 [in French].
Kumar, P., and Yadav, B.R. (1991). Comparative cytogenetical studies in Mehsana, Murrah and Surti buffaloes. Indian Journal of Dairy Sciences 46, 157-161.
Lin, C.C., Newton, D.P. and Church, R.B. (1977). Identification and nomenculture for G-banded bovine chromosomes. Canadian Journal of Genetics and Cytology 19, 281-283.
Meo, G.P., Perucatti, A., Floriot, S., Incarnato, D., Rullo, R., CaputiJambrenghi, A., Ferretti, L., Vonghia, G., Cribiu, E., Eggen, A. and Iannuzzi, L. (2005). Chromosome evolution and improved cytogenetic maps of the Y chromosome in cattle, zebu, river buffalo, sheep and goat. Chromosome Research 13, 349–355.
Mirhoseinie, S.Z FarhadVahidie, S.M. and Gharehyazie, B. (2005). Survey of efficiency of six microsatellite loci in Iranian indigenous cattle and buffalo populations. Iranian journal of biotechnology3, 41-47.
Miyake, Y., Kanagawa, H. and Ishikawa, T. (1980). A Chromosomal Analysis on The G and C Band Staining Techniques of The Buffalo (Bubalusbubalis). The Japanese Journal of Veterinary Research 28, 122-128.
Moorhead, P.Z., Novell, P.C., Mellman, W.J., Battis, D.M. and Hungerford, D.A. (1960). Chromosomal preparations of leukocytes cultured from human peripheral blood. Experimental cell research 20, 613-619.
Murrali, N., Devendran, P., and Panneerselvan, S. (2009). Cytogenetic studies on the chromosomes of Toda buffaloes. Buffalo Bulletin 28(2), 95-100.
Nair, P.G., Balakrishnan, M., Yadav, B.R. (1986). The Toda buffaloes of Nilgiris. Buffalo Journal 2, 167-178.
Naserian, A.A., and Saremi, B. (2007). Water buffalo industry in Iran. Italian journal of animal sciences 6, 1404-1405.
Pires, R.M.L., Reichert, R.H. and Kasahara, S. (1998).Cytogenetics of Three Breeds of River Buffalo (Bubalusbubalis L), with Evidence of a Fragile site on The X chromosome. Yheriogenology 49, 529-538.
Ramesha, K.P., and Hegde, B.P. (1992). Chromosomes of Surti and nondescript buffaloes of Kamataka. Indian veterinary journal 69, 34-37.
Toll, G.L. and Halnan, C.R.E. (1976a).The karyotype of the Australian swamp buffalo (Bubalusbubalis). Canadian Journal of Genetics and Cytology 18, 101-104.
Vijh, R.K., Tanita, M.S., and Sahai, R. (1994). Translocation in Murrah buffalo. Indian Journal of Animal Sciences 64, 534-538.
Yadav, B.R., Balakrishnan, C.R. and Tomer, O.S. (1984).Chromosomal screening of male cattle and buffaloes. Indian Journal of Animal Sciences 54, 519-523.