Detection of antibiotic residues in poultry carcasses in Mashhad poultry abattoir

Document Type : Research Articles


Ferdowsi University of Mashhad


In poultry breeding, use of any antibiotics as a food additive that promotes growth, prevention or treatment of infectious diseases will increase the chance of drug residues in poultry meat products.
In this study, the presence of different antibiotics in ten broiler flocks, which were later transported to Mashhad poultry abattoir have been investigated, using four plate test (FPT) method. A total of one hundred breast muscle and sixty liver samples (10 breast muscles and 6 liver samples from each flock) were collected randomly.
The results of this study showed, the 18.75% of samples were positive for antibiotic residues and 6.88% of samples were suspected. The results also showed that sulfonamides residue in meat and liver samples were higher than other investigated antibiotics (22% in meat and 11.7% in livers). It can be concluded that the withdrawal time of antibiotics before slaughter has been ignored in many of these farms therefore, the administration of antibiotics in poultry farms must be more rigidly controlled to prevent drug residues in food-producing animals.


Al-Ghamdi, M. S., Al-Mustafa, Z. H., El-Morsy, F., Al-Faky, A., Haider, I. and Essa, H. (2000) Residues of tetracycline compounds in poultry product in the eastern province of Saudi Arabia. Public Health 114, 300-304.
Cerina, S., Krastina, V. and Vitina, I. (2011) Production and expenses of enriched composition broiler chicken meat in Latvia. Agronomy Research 9, 383-388.
Charlton K. E., Probst Y., Tapsell L.C. and Blackall J. P. (2008) Food, health and nutrition: Where does chicken fit? Australian Chicken Meat Federatio (ACMF) Inc, publication p:1-19.
Dipeolu, M. A. and Alonge, D. O. (2002) Residues of streptomycin antibiotic in meat sold for human consumption in some states of SW Nigeria. Archivos de Zootecnica 51, 477-480.
Donoghue, Dan, J. (2003) Antibiotic residues in poultry tissues and eggs: human health concerns? Poultry Science 82, 618-621.
Givens, D, I. (2005) The role of animal nutrition in improving the nutritive value of animal derived foods in relation to chronic disease. Proceedings of the Nutrition Society. 64, 395-402.
Hussein, K., Marcinak, S., Mate, D., Kozarova, Ivona., Sokol, J. and Zdolec, N. (2005) Use of Premi test for the detection sulphonamide residues in chicken eggs. Acta Veterinaria (Beogard). 55 (5-6), 493-500.
Hussein, K. (2001) Experimental design for the microbiological four-plate test for the detection of Sulphadimidine residues at the levels of concern. The Bulletin of the Veterinary Institute in Pulawy 48, 403-407.
Ivanovic, S. (2003) Investigation of the influence of probiotics on the selected indicators of broiler’s meat hygiene and quality, PhD. Thesis, Faculty of Veterinary Medicine, Belgrade University, Serbia (in Serbian).
Javadi, A., Mirzaie, H. and Khatibi, S. A. (2011) Effect of roasting, boiling and microwaving cooking methods on Enrofloxacin residues in edible tissues of broiler. African Journal of Pharmacy and Pharmacology 5(2), 214-218.
Javadi, A., Mirzaie, H. and Khatibi, S. A. (2009) Effect of roasting process on antibiotic residues in edible tissues of poultry by FPT method. Journal of Animal and veterinary Advances 8(12), 2468-2472.
Kabir, J., Umoh, V. J., Audu-okoh, E., Umoh, J. U. and Kwaga, J. K. (2004) Veterinary drug use in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna State, Nigeria, Food Control 15(2), 99-105.
Karraouan, B., Bouchrif , B., Ziyate, N., Talmi, A., Yahia, Kh. I. S., Cohen, N and Fassouane, A. (2009) Evaluation of multi-plate microbial assay for the screening of antibacterial residues in poultry muscle. Europian Journal of Scientific Research 35(2), 311-317.
Kaya, S. E. and Filazi, A. (2010) Determination of antibiotic residues in milk samples. Kafkas Univ Vet Fak Derg 16, 31-35.
Kirbis, A. (2007) Microbiological screening method for detection of aminoglycosides, β-lactames, macrolides, tetracyclines and quinolones in meat samples. Slov Vet Res 44(1/2): 11-18.
Korsrud, G. O., Boison, J.O., Nouws, J.F. and MacNeil, J. D. (1998) Bacterial inhibition tests used to screen for antimicrobial veterinary drug residues in slaughtered animals. Journal of AOAC International. 81, 21-24.
Lemus, J. A., Blanco, G., Grande, J., Arroyo, B., Garcia-Montijano, M. and Martinez, F. (2008) Antibiotics threaten wildlife: circulating quinolone residues and disease in avian scavengers. PLoS ONE. 3(1), 1-6.
Lynas, L., Currie, D., Elliott, C. T., McEvoy, J. D. and Hewitt, S. A. (1998) Screening for chloramphenicol residues in the tissues and fluids of treated cattle by the four plate test, Charm II radioimmunoassay and Ridascreen CAP-Glucuronid enzyme immunoassay. Analyst. 123, 2773-2777.
Mahgoub, O., Kadim, I. T., Mothershaw, A., Al Zadjali, S. A. and Annamalai, K. (2006) Use of enzyme linked immunosorbent assay (ELISA) for detection of antbiotic and anabolic residues in goat and sheep meat. World Journal of Agricultural Sciences. 2 (3), 298-302.
Meredith, W. E., Weiser, H. H. and Winter, A. R. (1965) Chlortetracycline and Oxytetracycline residues in poultry tissues and eggs. Applied Microbiology 13(1), 86-88.
Myllyniemi, A. L. (2004) Development of microbiological methods for the detection and identification of antimicrobial residues in meat. National Veterinary and Food Research Institue. Helsinki, Finland.
Paryad, A. and Mahmoudi, M. (2008). Effect of different levels of supplemental yeast (saccharomyces cerevisiae) on performance, blood constituents and carcass characteristics of broiler chicks. African Journal of Agricultural Research 3(12), 835-842.
Reyes-Herrera, I., Schneider, M. J., Cole, K., Farnell, M. B., Blore, P. J. and Donoghue, D. J. (2005) Concentration of antibiotic residues vary between different edible muscle tissues in poultry. Journal of Food Protection 68(10), 2217-2219.
Saitanu, K., Amornsin, A., Kondo, F. and Tsai C. E. (1994). Antibiotic residues in tiger shrimp (Penaeus monodon). Asian Fisheries Science 7, 47-52. Stead, S., Sharman, M. and Tarbin, J. A. et al. (2004) Meeting maximum residue limits: an improved screening technigue for the rapid detection of antimicrobial residues in animal food products. Food Additive and Contaminants 21, 216-221.
Tajik, H., Maleknejad, H., Razavi-Rouhani, S. M., Pajouhi, R. Mahmoudi, M. R. and Haghnazari, A. (2010) Chloramphenicol residues in chicken liver, kidney and muscle: A comparison among the antibacterial residues monitoring methods of Four Plate Test, ELISA and HPLC. Food and Chemical Toxicology 48(8-9), 2464-2468.