Histopathological evaluation of the effect of platelet-rich fibrin on canine cutaneous incisional wound healing

Document Type : Research Articles


1 Research Institute , Mashhad

2 Islamic Azad University, Tabriz

3 Mashhad University of Medical Sciences

4 Ferdowsi University of Mashhad

5 Clinic of Dermatology, Mashhad


Platelets are a natural source of growth factors and cytokines that promote wound healing, and platelet-rich fibrin contains concentrated growth factors. Growth factors in platelet-rich fibrin may promote wound repair. In this study the effect of platelet-rich fibrin was evaluated in a dog model of cutaneous incisional wounds using histology and semi-quantitative evaluation. A pair of ten-centimeter full-thickness parallel linear cutaneous wounds were created on the backs of 15 dogs on the both sides of vertebral column. On the left side the platelet-rich fibrin clot was applied to the edges of the wound (Treatment group) and the right side received nothing (Control group). All wounds were then closed with 3-0 non-absorbable Nylon suture. The dogs were divided into three groups of five dogs. The wound tissues were sampled by electrosurgery in group one after 3 days, group two after 7 days and group three after 14 days post surgery. For each specimen, histopathological examination and semi-quantitative evaluation was performed by light microscopy using Hematoxylin & Eosin and Masson’s trichrome staining. The results demonstrated that platelet-rich fibrin improved and accelerated cutaneous incisional wound healing.


Bancroft, J., and Stevens, A. (1982) Textbook of Theory and practice of histological techniques, 2nd edn., pp 20-61.
Dohan, M., Choukroun, J., Diss, A., Dohan, S., Dohan, A., Mouhyi, J. (2006) Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Oral Surgery, Oral Medicine, Oral pathology, oral Radiology, and Endodontology 101, 37-44.
Dohan, M., Del Corso, M., Diss, A., Mouhyi, J., Charrier, J. (2010) Three-Dimensional Architecture and Cell Composition of a Choukroun’s Platelet-Rich Fibrin Clot and Membrane. Journal of periodontal 81, 546-555.
Dohan, M., Rasmusson, L., Albrektsson, T. (2009) Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte - and platelet-rich fibrin (L-PRF). Trends in Biotechnology 27, 158-167.
Fossum, T., Hedland, C., Hulse, D., Johnson, A. (2002) Textbook of Small Animal Surgery, 2nd edn., Mosby. pp 134-137, 255-258.
Gal, P., Toporcer, T., Vidinsky, B., Mokry, M., Novotny, M., Kilik, R. (2006) Eearly changes in the Tensile strength and morphology of primary sutured skin wounds in Rats. Folia Biologica 52, 110-115.
Hichman, J., Houlton, J., Edwards, B. (1995) An Atlas of Veterinary surgery, 3rd edn., Blackwell science. pp 63-68.
Kimura, A., Ogata, H., Nakajima, T., Yazawa, M., Watanabe, N., Mori, T. (2005) The effects of platelet–rich plasma on cutaneous incisional wound healing in rats. Journal of Dermatological Science 40, 205-208.
Pallua, N., Wolter, T., Markowicz, M. (2010) Platelet-rich plasma in burns. Journal of burns 36, 4-8.
Raja, s., Naidu, M. (2008) Platelet-rich fibrin: Evolution of a second –generation platelet concentrate. Indian Journal of Dental Research 19, 42-47.