Antifungal and toxicity effects of new combined essential oils on Oncorhynchus mykiss in comparison with malachite green

Document Type : Research Articles


1 1Department of Fisheries, Faculty of Marine Natural Resources, Khoramshahr Marine Sciences and Technology University, Khoramshahr

2 2Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran

3 Department of Horticulture, Faculty of Agriculture, University of Tarbiat Modarres, Tehran

4 4Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran

5 5Department of Food Control & Hygen, Faculty of Veterinary Medicine, University of Tehran, Tehran


Mold infection is one of the most important problems in aquaculture. Recently, administration of some chemicals such as malachite green in order to control mold infections has been limited in aquaculture. In the current decade, researchers have been more focused on using herbal extracts and essential oils in aquaculture. In this study, Minimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) of a new combination of four essential oils (Eucalyptus globulus, Mentha piperita, Salvia officinalis and Thymus vulgaris) were determined by serial dilution method. LC50 of the combined essential oils for 48 and 96 hours were then determined on Oncorhynchus mykiss fingerlings. MIC and MFC of combined essential oils were 0.025μl/ml and 0.050μl/ml for F. solani and 0.018μl/ml and 0.035μl/ml for S. parasitica, which was lower compared to malachite green. It was also 0.060μl/ml and 0.300μl/ml for F. solani and 0.045μl/ml and 0.120μl/ml for S. parasitica respectively (p


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