Lipid rich carcinoma and solid carcinoma in mammary gland of a dog: histopathologic and immunohistochemical features

Document Type : Research Articles

Authors

Shahid Chamran University of Ahvaz

Abstract

Lipid rich carcinoma is rare in dog and women and is characterized by cells that have an abundant foamy cytoplasm. A 10 year old, mix breed female dog suffered two subcutaneous masses in ventral abdomen was referred to surgery department of veterinary hospital of Ahvaz Shahid Chamran University. Surgical excision and microscopic examination of masses were done. After grading by Elston and Ellis method, istochemical staining was performed such as PAS, Cong red and Oil Red O.Immunohistochemical detection of pKi67, P53, cerbB2
and factor VIII – related antigen were carried out.icroscopically, the masses were two parts; first compose of big cells with vacuolated cytoplasm and euchromatin nuclei and
second part cells with scant cytoplasm and pleomorphic nuclei and obvious membrane. Grade of tumor was Grade III, poorly differentiated. Positive reaction was seen in vacuolated
cytoplasm for Oil Red O. Neoplastic cells of lipid rich area demonstrated intense immunoreactivity for pKi67. All tumor cells lacked p53 and c-erbB2. This is the first report of coincidence of lipid rich carcinoma and solid carcinoma in a dog with description of
histopathologic and immunohistochemical characteristics.

Keywords


Chang S C, Liao J W, Wong M L, Lai Y S and Liu C I (2007) Mammary carcinoma with sebaceous differentiation in a dog.
Veterinary pathology, 44, 525-527.
Chen J, Zhang J, Liang Q, Bao Z, Zheng Z, Hu N and Yan H (2007) Bilateral breast cancer with a unilateral lipid – rich
carcinoma: a case report. Chinese Journal of clinical oncology, 4, 376-377.
Espinosa De Los Monteros A, Hellmen E,Ramirez G A, Herraez P, Rodriguez F,Ordas J, Millan Y, Lara A and Martin de las Mulas J (2003) Lipid – rich carcinoma of the mammary gland in
seven dogs: clinicopathologic and immunohistochemical features.
Veterinary Pathology, 40, 718-723.
Kamstock D A, Fredrickson R and Ehrhart E J (2005) Lipid – rich carcinoma of the mammary gland in a cat. Veterinary
Pathology 42, 360-362.
Luong R H, Baer K E, Craft D M, Ettinger S N, Scase T J and Bergman P J (2006) Prognostic significance of intratumoral
microvessel density in canine soft – tissue sarcomas. Veterinary Pathology,43, 622-631.
Misdorp W (2002). Tumors of the mammary gland. In Meuten, D. J. Tumors in domestic animals. 4th edition, A Blackwell Publishing Company ,Iowa state press, pp: 575-606.
Misdorp W, Else, RW, Hellmen E and Lipscomb TP. (1999). Histological Classification of Mammary Tumors of the Dog and the Cat. Vol VII, Armed Forces Institute of Pathology, American Registry of Pathology, Washington, DC, and the World Health
Organization Collaborating Center for Worldwide Reference on Comparative Oncology, pp. 1–59.
Pena L, Perez-Alenza M D, Rodriguez-Bertos A and Nieto A (2003) Canine inflammatory mammary carcinoma: histopathology, immunohistochemistry and clinical implications of 21 cases.
Breast cancer research and technology, 78, 141-148.
Perez – Martinez C, Garcia – Iglesias M J,Duran – Navarrete A J, Espinosa-Alvarez J, Garcia-Fernandez RA,Lorenzana-Robles N, Fernandez-Perez S and Garcia-Marin JF (2005) Histopathological and immunohistochemical characteristics of two canine lipid – rich mammary carcinomas. Journal of Veterinary Medicine, series A, 52, 61-66.
Rezaie A, Tavasoli A, Bahonar A and Mehrazma M (2009) Grading in canine mammary carcinoma. Journal of Biological sciences, 9, 333-338.
Rezaie A, Tavasoli A, Mehrazma M and Bahonar A (2011) Immunohistochemical staining expression of Ki67 protein in canine mammary gland carcinoma. Comparative clinical pathology, 20 (6), 557-561.
Shi P, Wang M, Zhang Q and Sun J (2008) Lipid – rich carcinoma of the breast. A clinicopathological study of 49 cases.
Tumori, 94, 342-346.
Yoshimura H, Kimura N, Nakahira R,Michishita M, Ohkusu – Tsukada K and Takahashi K (2010) Lipid – rich carcinoma in the mammary gland of a Djungarian hamster (Phodopus
sungorus). Journal of veterinary diagnostic investigation, 22, 305-309.