Dirofilaria immitis infection in a Dachshund dog: Diagnosis and Treatment

Document Type : Research Articles


Shahid Chamran University of Ahvaz


Dirofilaria immitis is a filariid worm which typically lives as adult in the right ventricle of the heart and the pulmonary arteries of the dog, causing the canine heartworm disease.Therapy of canine dirofilariosis due to Dirofilaria immitis is indicated for dogs suffering
from clinical signs of the disease (such as chronic cough). The present paper describes diagnostic and treatment features of a D. immitis affected dog in Ahvaz district, Southwest of
Iran. The dog had coughing, tachypnea, labored breathing, panting, hind limb edema,weakness, and exercise intolerance, at the time of referral. Auscultation revealed grade III/VI
systolic murmur over the left apex of the heart. Giemsa stained blood smears containing microfilaria was morphologically identified as D. immitis. CBC revealed an inflammatory leukogram and mild anemia. Combined therapy with ivermectin (440 μg/kg as single dose),levamisol (10 mg/kg q 24 h for 10 days) and aspirin (10 mg/kg q 12 h for 10 days), during 2 stages, was effective. The number of microfilaria dropped from 1250±50/ml blood pretreatment to 150±10/ml (following the first treatment stage, after 10 days) and reached 0 a day after the second treatment. The animal remained negative regarding D. immitis microfilaremia
during a follow-up period of 180 days. This record confirms the efficacy of ivermectin with levamisol and aspirin in the treatment of adult heartworm infection in a dog.


Main Subjects

Arther, R.G., Bowman, D.D., Slone, R.L. and Travis, L.E. (2005) Imidacloprid plus moxidectin topical solution for the prevention of heartworm disease (Dirofiloria immitis) in dogs.Parasitology Research 97, 76-80.
Bazzocchi, C., Mortarino, M., Grandi, G.,Kramer, L.H., Genchi, C., Bandi, C.,Genchi, M., Sacchi, L. and McCall,J.W. (2008) Combined ivermectin and doxycycline treatment has microfilaricidal and adulticidal activity against Dirofilaria immitis in
experimentally infected dogs.International Journal for Parasitology 38,1401-1410.
Bourguinat, C., Keller, K., Bhan, A.,Peregrine, A., Geary, T. and Prichard,R. (2011) Macrocyclic lactone resistance in Dirofilaria immitis.Veterinary Parasitology 181, 388-392.
Carter, J.E., Chanoit, G. and Kata, C. (2011) Anaphylactoid reaction in a heartworm-infected dog undergoing lung lobectomy. Journal of the American Veterinary Medical Association 238, 1301-1304.
Cunningham, C.P., Brown, J.M., Jacobson,G.A., Brandon, M.R. and Martinod, S.R. (2006) Evaluation of a coveredrod silicone implant containing ivermectin for long-term prevention of
heartworm infection in dogs. American Journal of Veterinary Research 67,1564-1569.
Dantas-Torres, F., Lia, R.P., Barbuto, M.,Casiraghi, M., Crovace, A., Caligiani,L., Genchi, C. and Otranto, D. (2009) Ocular dirofilariosis by Dirofilaria immitis in a dog: first case report from
Europe. The Journal of Small Animal Practice 50, 667-669.
Dillon, R., Brawner, W.R. and Hanrahan, L.(1995) Influence of number of parasites and exercise on the severity
of heartworm disease in dogs.Proceedings of the ’95 Heartworm
Symposium, American Heartworm Society, Batavia, IL. p 113-120.
Di Sacco, B. and Vezzoni, A. (1992) Clinical classification of heartworm disease for the purpose of adding objectivity to the
assessment of therapeutic efficacy of adulticidal drugs in the field. Proceedings of the Heartworm Symposium ’92, American Heartworm Society, Batavia. p 209-214.
Grandi, G., Quintavalla, C., Mavropoulou, A.,Genchi, M., Gnudi, G., Bertoni, G. and Kramer, L. (2010) A combination of
doxycycline and ivermectin is adulticidal in dogs with naturally
acquired heartworm disease (Dirofilaria immitis). Veterinary
Parasitology 169, 347-351.
Greene, C.E. (2006) Infectious Diseases of the Dog and Cat. 3rd edition, W.B.Saunders Co. Philadelphia.Kamalu, B.P. (1991) Canine filariasis caused by Dirofilaria repens in southeastern
Nigeria. Veterinary Parasitology, 40,335-338.
Knight, D.H. (1995). Guidelines for diagnosis and management of heartworm (Dirofilaria immitis) infection. In: J.D.Bonagura (Ed). Kirk’s Current Veterinary Theraphy XII Small Animal Practice. W.B. Saunders Co.
Lee, A.C., Montgomery, S.P., Theis, J.H.,Blagburn, B.L. and Eberhard, M.L.(2010) Public health issues concerning the widespread distribution of canine h e a r t w o r m disease. Trends in Parasitology 26, 168-173.
McCall, J.W., Guerrero, J., Roberts, R.E., Supakorndej, N., Mansour, A.E.,Dzimianski, M.T. and McCall, S.D.(2001). Further evidence of clinical prophylactic (reachback) and adulticidal activity of monthly administration of ivermectin and
pyrantel pamoate in dogs experimentally infected with heartworms. In L. Seward (Ed).Proceedings of the American
Heartworm Symposium ‘01. American Heartworm Society, Batavia, IL. pp 189-200.
Merawin, L.T., Arifah, A.K., Sani, R.A.,Somchit, M.N., Zuraini, A., Ganabadi, S. and Zakaria, Z.A. (2010) Screening of microfilaricidal effects of plant extracts against Dirofilaria immitis.
Research in Veterinary Science 88,142-147.
Oliveira, C., Rademacher, N., David, A.,Vasanjee, S. and Gaschen, L. (2010) Spontaneous pneumothorax in a dog
secondary to Dirofilaria immitis infection. Journal of Veterinary
Diagnostic Investigation 22, 991-994.
Otranto, D., de Caprariis, D., Lia, R.P.,Tarallo, V., Lorusso, V., Testini, G.,Dantas-Torres, F., Latrofa, S., Diniz, P.P., Mencke, N., Maggi, R.G.,Breitschwerdt, E., Capelli, G. and Stanneck, D. (2010) Prevention of endemic canine vector-borne diseases
using imidacloprid 10% and permethrin 50% in young dogs: a
longitudinal field study. Veterinary Parasitology 172, 323-332.
Pollono, F., Pollmeier, M. and Rossi, L.(1998). The prevention of Dirofilaria repens infection with ivermectin/pyrantel chewables.
Parassitologia 40, 457-459.
Rohrbach, B.W., Lutzy, A. and Patton, S.(2011) Attributes, knowledge, beliefs,and behaviors relating to prevention of
heartworm in dogs among members of a national hunting dog club. Veterinary Parasitology 176, 324-332.
Rossi, M.I., Paiva, J., Bendas, A., Mendes-de-Almeida, F., Knackfuss, F., Miranda,M., Guerrero, J., Fernandes, O. and
Labarthe, N. (2010) Effects of doxycycline on the endosymbiont
Wolbachia in Dirofilaria immitis (Leidy, 1856)-naturally infected dogs.Veterinary Parasitology 174, 119-123.
Schnieder, T., Lechler, M., Epe, C.,Kuschfeldt, S. and Stoye, M. (1996) The efficacy of doramectin on arrested larvae of Ancylostoma caninum in early pregnancy of bitches. Zentralblatt
Veterinarmedizin 43, 351-356.
Schnyder, M., Tanner, I., Webster, P.,Barutzki, D. and Deplazes, P. (2011) An ELISA for sensitive and specific detection of circulating antigen of Angiostrongylus vasorum in serum
samples of naturally and experimentally infected dogs.
Veterinary Parasitology 179, 152-158.
Tarello, W. (2002) Dermatitis associated with Dirofilaria (Nochtiella) repens microfilariae in dogs from central Italy.
Acta Veterinaria Hungarica 50, 63-78.
Venco, L., McCall, J.W., Guerrero, J. and Genchi, C. (2004) Efficacy of longterm monthly administration of ivermectin on the progress of naturally acquired heartworm infections in dogs.
Veterinary Parasitology 124, 259-268.
Vezzoni, A., Genchi, C. and Raynaud, J.P.(1992) Adulticide efficacy of RM 340 in dogs with mild and severe natural
infections. In: M.D. Soll (Ed).Proceedings of the Heartworm
Symposium ’92, American Heartworm Society, Batavia, IL. pp 231-240.