Variations of Energy Related Biochemical Metabolites During Periparturition Period in Fat-Tailed Baloochi Breed Sheep

Document Type : Research Articles

Authors

Ferdowsi University of Mashhad

Abstract

Negative energy balance in pregnant ewes during last two month of gestation could result in fatal pregnancy toxemia and affects ewe and lamb health. The understanding of variations of energy related metabolites during this critical period may cast light upon to a better management of the situation. This study describes the variations of serum energy related biochemical metabolites at a period of 45 days before to 21 days after parturition in Baloochi breed ewes. Blood from the jugular vein was collected from each sheep at days 45, 30, 20, 10, 1 prior to parturition, and days 4, 7, 13, 19 and 21 post-partum to determine total protein, albumin, urea , cholesterol, glucose, triglyceride, aspartate aminotransferase (AST), -Hydroxybutyrate (BHBA) and non-esterified fatty acid (NEFA). NEFA and BHBA concentrations reached peak levels at parturition. However, AST activity had highest levels at the first and second week after lambing. AST activity significantly correlated to energy metabolism indicators. This enzyme had positive significant correlations with NEFA and BHBA and negative correlations with cholesterol and triglyceride. NEFA concentrations had a positive correlation with BHBA and negative correlation with glucose and cholesterol. At pre-partum period NEFA correlation with BHBA and AST were stronger than post-partum. This finding indicates that NEFA testing at pre-parturition days is a reliable predictor of fat mobilization and energy status of pre-parturition period in sheep. Urea and total protein concentrations reached lowest levels at parturition and then gradually increased afterwards.

Keywords


Al-Dewachi, O.S. (1999) Some biochemical constituents in the blood serum of pregnant Awassi ewes. Iraqi Journal of Veterinary
Science, 12, 275–279.
Balikci, E., Yildiz, A. and Gurdogan, F. (2007) Blood metabolite concentrations during pregnancy and postpartum in Akkaraman
ewes. Small Ruminant Research, 67, 247–251.
Bauman, D.E. and Currie, W.B. (1980) Partitioning of Nutrients during pregnancy and lactation: A review of mechanisms
involving homeostasis and hemeorhesis.Journal of Dairy Science, 63, 1514-1529.
Bertics, J.S., Grummer, R.R., Cadorniga-Valino, C. and Stoddard, E. E. (1992) Effect of prepartum dry matter intake on liver triglyceride concentration and early lactation. Journal of Dairy Science, 75,1914.
Charismiadou, M.A., Bizelis, J.A. and Rogdakis, E. (2000) Metabolic changes during the perinatal period in dairy sheep
in relation to level of nutrition and breed. I.Late pregnancy. Journal of Animal Physiology Animal. Nutrition, 84, 61–72.
Edmondson, M.A.and Pugh, D.G. (2009) Pregnancy toxemia in sheep and goats. In Anderson, D.E., Rings, D.M., editor:
Current veterinary therapy food animal practice, Philadelphia, Saunders, pp. 144-145.
Hamadeh, M. E., Bostedt, H. and Failing, K.(1996) Concentration of metabolic parameters in the blood of heavily pregnant and nonpregnant ewes. Berliner und Munchener tierarztliche
Wochenschrift, 109, 81–86.
Henze, P., Bickhardt, K. and Fuhrmann, H.(1994) The influences of insulin, cortisol,growth hormone and total oestrogen on the
pathogenesis of ketosis in sheep. Deutsche Tierarztliche Wochenschrift, 101, 61–65.
Herdt, T. H. (1988) Fatty liver in dairy cows.Veterinary Clinics of North America: Food Animal Practice 4, 269-287.
Herdt, T. H. and Gerloff, B. J. (2009) Ketosis.In Current Veterinary Therapy Food Animal Practice, 5th ed., Anderson, D.E.
and Rings, D.M. (Eds.), Saunders, St.Louis, pp. 141-144.
Kaneko, J. J., Harvey, J. W. and Bruss, M. L.(2008) Clinical biochemistry of domestic animals, 6th edn. Elsevier Academic,
Amsterdam, pp 356–365.
Khan, A., Bashir, M., Ahmad, K. M., Javed,M. T., Yayyab, K. M. and Ahmad, M.(2002) Forecasting neonatal lamb mortality
on the basis of haematological and enzymological profiles of Thalli ewes at the pre-lambing stage. Small Ruminant Research 43, 149-156.
Jainudee, M. R. and Hafez, E. S. E. (1994) Gestation, prenatal physiology and parturition. In: Hafez, E.S.E. (Ed.),Reproduction in Farm Animals. Lea and Febiger, Philadelphia, pp. 247–283.
LeBlanc, S. J. (2006) Monitoring programs for transition dairy cows. Proceeding of 26th World Biuatrics Congress, Nice, 460-472.
Navarre, C. B. and Pugh, D. G. (2002) Diseases of the gastrointestinal system. In Pugh, D. G. editor: Sheep and goat
medicine, Philadelphia, Saunders, pp. 97-99.
Reid, I. M., Rowlands, G. J., Dew, A.M.,Collins, R. A. Roberts, C. J. and Manston,R. (1983) The relationship between postpartum
fatty liver and blood compositions in dairy cows. Journal of Agricultural Science 101, 473-480.
Sattari, M. (1988) Sheep Husbandry in Iran:Sheep Breeds. Tehran University Press,Tehran. pp. 184-225.
Seifi, H. A., Gorji-Dooz, M., Mohri, M., Dalirnaghadeh,B. and Farzaneh, N. (2007) Variations of energy-related biochemical
metabolites during transition period in dairy cows. Comparative Clinical Pathology 16, 253-258.
Shetaewi, M. M. and Daghash, H. A. (1994) Effects of pregnancy and lactation on some biochemical components in the blood of Egyptian coarse-wool ewes. Association Veterinary Medicine Journal 30, 64–73.
Sommer, H. (1995) The role of metabolic profile test in the control of cattle feeding.Magyar Allatorvosok Lapja (Hungrian
Veterinary Journal) 50, 714-717.
Taghipour, B., Seifi, H. A., Mohri, M.,Farzaneh, N. and Naserian, A. A. (2010) Effect of prepartum administration of monensin on metabolism of pregnant ewes. Livestock Science.
doi:10.1016/j.livsci.2010.07.015
Takarkhede, R .C., Gondane, V. S., Kolte, A.Y. and Rekhate, D. H. (1999) Biochemical profile during different phases of
reproduction in ewes in comparison to rams. Indian Veterinary Journal 76, 205–207.
Watson, T. D. G., Burns, L., Packard, C. J. and Shepherd, J. (1993) Effects of pregnancy and lactation on plasma lipid and
lipoprotein concentrations, lipoprotein composition and post-heparin lipase activities in Shetland pony mares. Journal
of Reproduction and Fertility 97, 563-568.