The Effect of Storage on the Protein Electrophoretic Pattern in Bovine Serum

Document Type : Research Articles


University of Veterinary Medicine and Pharmacy, Komenského 73


This study was aimed at the evaluation of the influence of storage under various conditions
on the relative concentrations of major protein fractions and their proportion in bovine serum.
Blood samples were taken from six dairy cattle of a low-land black spotted breed and its
crossbreeds. The separated blood serum was fractioned into aliquots. One aliquot was
analysed immediately after the separation without storage. The second aliquot was stored at 4
°C for 1 day, the remaining aliquots were kept frozen at -18 °C for 2, 7, and 21 days, and then
analysed. Blood serum was analysed by agarose gel electrophoresis for the major protein
fractions – albumin (%), alpha-globulins (%), beta-globulins (%), and gammaglobulins (%).
Over time, the relative concentrations of albumin in bovine serum showed a tendency of
significant decrease during the storage at -18 °C (p


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