The Effect of Storage on the Protein Electrophoretic Pattern in Bovine Serum

Document Type : Research Articles

Authors

University of Veterinary Medicine and Pharmacy, Komenského 73

Abstract

This study was aimed at the evaluation of the influence of storage under various conditions
on the relative concentrations of major protein fractions and their proportion in bovine serum.
Blood samples were taken from six dairy cattle of a low-land black spotted breed and its
crossbreeds. The separated blood serum was fractioned into aliquots. One aliquot was
analysed immediately after the separation without storage. The second aliquot was stored at 4
°C for 1 day, the remaining aliquots were kept frozen at -18 °C for 2, 7, and 21 days, and then
analysed. Blood serum was analysed by agarose gel electrophoresis for the major protein
fractions – albumin (%), alpha-globulins (%), beta-globulins (%), and gammaglobulins (%).
Over time, the relative concentrations of albumin in bovine serum showed a tendency of
significant decrease during the storage at -18 °C (p

Keywords


Bossuyt, X., Schiettekatte, G., Bogaerts, A.,Blanckaert, N. (1998) Serum protein electrophoresis by CZE 2000 clinical
capillary electrophoresis system. Clinical Chemistry 44, 749-759.
Boyanton, B., Blick, K.E. (2002) Stability studies of twenty-four analytes in human plasma and serum. Clinical Chemistry 48,
2242-2247.
Cray, C., Rodriguez, M., Zaias, J., Altman, N.(2009) Effects of storage temperature and time on clinical biochemical parameters
from rat serum. Journal of the American Association for Laboratory Animal Science 48, 202-204.
Ehsani, A., Afshari, A., Bahadori, H., Mohri,M., Seifi, H.A. (2008) Serum constituents analyses in dairy cows: Effects of duration
and temperature of the storage of clotted blood. Research in Veterinary Science 85,473-475.
Chaudhary, Z.I., Iqbal, J., Rashid, J. (2003) Serum protein electrophoretic pattern in young and adult camels. Australian
Veterinary Journal 81, 625-626.
Jakubowski, J., Luetzelschwab, J., Aebischer,V., Vogel, B., Donatsch, P., Cordier, A.(1998) Stability of clinical chemistry
parameter values in minipig serum under different storage conditions. Scandinavian Journal of Animal Sciences 25 (Suppl. 1),
197-204.
Jenkins, M.A., Guerin, M.D. (1995) Quantification of serum proteins using capillary electrophoresis. Annals of
Clinical Biochemistry 32, 493-497.
Jonsson, M., Carlson, J. (2002) Computersupported interpretation of protein profiles after capillary electrophoresis. Clinical Chemistry 48, 1084-1093.
Luraschi, P., Brambilla, S., Infusino, I.,Franzini, C. (2003) Precision and longterm stability of capillary electrophoresis
measurement of serum protein zones.Clinica Chimica Acta 333, 41-45.
Marshall, W.J., Bangert, S.K. (2008) Clinical biochemistry: metabolic and clinical aspects, 2nd edn., Churchill Livingstone,
Elsevier, Philadelphia, USA. pp 7-16.
Medaille, C., Briend-Marchal, A., Braun, J.P.(2006) Stability of selected hematology variables in canine blood kept at room
temperature in EDTA for 24 and 48 hours.Veterinary Clinical Patholology 35, 18-23.
Riond, B., Wenger-Riggenbach, B., Hofmann-Lehmann, R., Lutz, H. (2009) Serum protein concentrations from clinically
healthy horses determined by agarose gel electrophoresis. Veterinary Clinical Patholology 38, 73-77.
Sharma, P. (2009) Preanalytical variables and laboratory performance. Indian Journal of Clinical Biochemistry 24, 109-110.
Schram, J.L., Pearson, R.E. (2005) Array analysis of human whole blood changes in protein fractions. Available at
www.iwdtechnology.com Trumel, C.S.F., Braun, J.P., Guelfi, J.F. (1996) Serum protein electrophoresis: guidelines for diagnosis evaluation in the dog, cat, and horse. Revue de Medicine Veterinaire 147, 123-130.
Wijnen, P.A., van Dieijen-Visser, M. (1996)Capillary electrophoresis of serum proteins: Reproducibility, comparison with agarose gel electrophoresis and a review of the literature. European Journal of Clinical Chemistry and Clinical Biochemistry 34, 535-545.