Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001Antifungal and toxicity effects of new combined essential oils on Oncorhynchus mykiss in comparison with malachite green182644910.22067/veterinary.v4i2.9457ENMohammad Mousavi1Department of Fisheries, Faculty of Marine Natural Resources, Khoramshahr Marine Sciences and Technology University, Khoramshahr0000-0002-7366-8808Seyed Saeed Mirzargar2Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, TehranHosseinali Ebrahimzadeh Mousavi2Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, TehranReza OmidbaigiDepartment of Horticulture, Faculty of Agriculture, University of Tarbiat Modarres, TehranAlireza Khosravi4Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, TehranAlireza Bahonar5Department of Food Control & Hygen, Faculty of Veterinary Medicine, University of Tehran, Tehran0000-0002-0846-282XJournal Article20110818Mold infection is one of the most important problems in aquaculture. Recently, administration of some chemicals such as malachite green in order to control mold infections has been limited in aquaculture. In the current decade, researchers have been more focused on using herbal extracts and essential oils in aquaculture. In this study, Minimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) of a new combination of four essential oils (Eucalyptus globulus, Mentha piperita, Salvia officinalis and Thymus vulgaris) were determined by serial dilution method. LC50 of the combined essential oils for 48 and 96 hours were then determined on Oncorhynchus mykiss fingerlings. MIC and MFC of combined essential oils were 0.025μl/ml and 0.050μl/ml for F. solani and 0.018μl/ml and 0.035μl/ml for S. parasitica, which was lower compared to malachite green. It was also 0.060μl/ml and 0.300μl/ml for F. solani and 0.045μl/ml and 0.120μl/ml for S. parasitica respectively (phttps://ijvst.um.ac.ir/article_26449_83327801f4ce7c424a65d95c78d090fe.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001A histological survey of the brain development in the sturgeon (Acipenser gueldenstaedtii)9162647310.22067/veterinary.v4i2.9931ENAli Akbar Rahmani AmoliGraduated student, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranYaser ToosizadehGraduated student, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranZohreh SaadatfarDepartment of Anatomical Science, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranJournal Article20111001Brain development of the Acipenser gueldenstaedtii (A.gueldenstaedtii) was described from days 1 to 36, post-hatch (dph) in this study. Larvae were euthanized, and serial sections of specimens were examined. Our ontogenical study was determined to concentrate on important parts of the brain containing forebrain, midbrain and hindbrain. The primary observation of the telencephalon was in 1-week-old specimens. The size of the forebrain remained relatively steady and did not demonstrate considerable changes, as compared to the other parts of brain. The diencephalon changes were noticeable in 3-week-old A.gueldenstaedtii. 2 major parts of mesencephalon, (tegmental portion and optic tectum) were detected during 4 weeks of development. As in other chondrosteans, the cerebellum appeared highly developed. Medulla oblongata appeared larger in 1-week-old specimens than in 4-week-old specimens.https://ijvst.um.ac.ir/article_26473_8b6f98b8edf7326ccaf149e7d302b1cd.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti – Fasciola gigantica antibodies in sheep17242657110.22067/veterinary.v4i2.16169ENMohammad Hossein Razi JalaliDepartments of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz0000-0002-8160-8522Hossein HamidinejatDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, AhvazMassoud GhorbanpoorDepartments of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, AhvazSaleh EsmaeelzadehDepartments of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, AhvazAbbas SohbatiGraduated of parasitology, Faculty of veterinary medicine , Shahid Chamran u nivercity , AhvazJournal Article20121020Tropical fasciolosis caused by infection with Fasciola gigantica is regarded as one of the most important single helminthes infections of ruminants in Asia and Africa. Fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. To evaluate the usefulness of ELISA for diagnosis of anti – Fasciola gigantica antibodies in parasitized sheep, crude, as well as excretory-secretory (E/S) antigens were examined with 50 positive and 45 negative serum samples. Results were compared with liver inspection in slaughterhouse. The cut off values were determined in 0.469 and 0.516 for crude and E/S antigen respectively. We found 82% of sensitivity, 95.6 of specificity, 95.3% positive predictive value and 83 % negative predictive value using crude antigen. We have also found 92% of sensitivity, 93% specificity, 93.8% positive predictive value and 91.4 % negative predictive value using E/S antigen. We concluded that ELISA, specially using E/S antigen, is a good tool for serodiagnosis of Fasciola gigantica infection in sheep herds.https://ijvst.um.ac.ir/article_26571_a3e05789909c4162baf685dd5e0e1c3f.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001A survey of Nosema apis infection in apiaries of North Khorasan province, Iran25302659810.22067/veterinary.v4i2.17418ENAli MoshaveriniaDepartment of Pathobiology, Faculty of veterinary medicine, Ferdowsi University of Mashhad0000-0002-3466-3502Vali Abedi2Veterinary office of Esfarayen, North Khorasan provinceHassan SafaeiVeterinary Office of Esfarayen,IranJournal Article20121203Nosema apis is an obligate intracellular parasite belonging to phylum Microsporidia. This para site is known as main causative agent of nosemosis in honeybees especially in the European honeybee (Apis mellifera). Nosemosis can cause queen supersedure, reduction of honey yield and dwindling the population of honeybees. A total number of 54 apiaries were randomly sampled from April to July 2011 and April to July 2012 in North Khorasan province, Iran. Collected samples were examined for infection to N.apis. In the collected bees from 30 apiaries, any Nosema infection was not observed in spring 2011, while of 24 apiaries sampled in spring 2012, 12 (50%) apiaries were infected with N. apis. The seasonal rainfall of spring in 2012 in the study area was 53% higher than the same period of last year. It was concluded that there has been a direct relationship between rainfall and frequency of N. apis infection in apiaries of North Khorasan province.https://ijvst.um.ac.ir/article_26598_2fbdec726b24bfa154e208bf9cefdef9.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001Influence of feed contaminated with seeds of cleavers (Galium aparine) on the health and production traits in broiler chickens31362662110.22067/veterinary.v4i2.16152ENIvan DinevDepartment of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora0000-0002-0197-3491Journal Article20121019Clinical observations and gross pathological studies have been performed in field cases in order to determine the effect of cleavers (Galium aparine) seeds upon the productive traits and the health of broiler chickens. A negative impact upon productive traits (feedconversion ratio, growth rates and equalization) has been observed. The results of our studies allowed assuming that the health of broiler chickens was influenced by the mechanical ileus due to deposition and accumulation of weed seeds in the gastrointestinal tract of chickens.https://ijvst.um.ac.ir/article_26621_271d81ffa0cf060ea1394fbc251a3b29.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001A survey of ecto- and endo-parasites of domestic pigeons (Columba livia) in Mashhad, Iran37422664510.22067/veterinary.v4i2.3215ENHasan BorjiDepartment of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran0000-0003-4706-4995Elham MoghaddasDepartment of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranGholam Reza RazmiDepartment of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran0000-0002-0754-1278Mahbobeh AzadDepartment of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranJournal Article20100707As there is no data available on parasite infection of pigeons (Columba livia) in the region, the present study was carried out on domestic pigeons in Mashhad city in the north-east of Iran. Three hundred specimens were examined for the presence of ecto and endoparasites. A total of 300 pigeons, %21.6(n=65) and %15.3(n=46) were infected with nematodes and cestodes respectively whereas no trematodes were found. The helminths and their prevalence were Nematoda: Ascaridia columbae (20.37%), Capillaria bursata (3.7%), Capillaria caudinfillata (1.85%), Eulimdana clava (2.7%), Gongylonema spp (0.9), Cestoda: Choanotaenia infundibulum (11.11%), Raillietina echinobothrida (6.48%). Six different species of ectoparasites: Columbicloa columbae (42.8%), Pseudolynchia canariensis (16.1%), Menocanthus stramineus (10.7%), Menopon gallinae (7.1%), Lipeurus caponis (5.3%), Argas reflexus (3.5%) and 2 haemoparasites species Haemoproteus columbae (50%) and Leucocytozoon spp. (2%) were identified. Further studies are recommended in assessing the effects of the parasites on the pigeons’ health and production.https://ijvst.um.ac.ir/article_26645_88261487b33cc7d8df4c0cb1a619edd1.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001Polydipsia/polyuria syndrome in a Congo African grey parrot (Psittacus erithacus erithacus):a case report43482668410.22067/veterinary.v4i2.15357ENJamshid RazmyarDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.0000-0002-1247-4591Mohammad HeidarpourDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.0000000205807984Masoud RajabiounDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.0000-0002-8881-2024Journal Article20120917Polydipsia/polyuria syndrome is not a common manifestation in birds. Hyperglycemia was found on plasma chemistry in an African Grey Parrot (Psittacus erithacus), which was presented with polyphagia and self mutilation symptoms. Radiography demonstrated signs of mild renalomegaly, air sac involvement which proved to be due to mycoplasma spp. infection on bactriological examination. The polydipsia, polyphagia and polyuria were treated successfully by insulin regime but self mutilation around the neck was to be most consistent sign for 45 days since the bird presented to our Faculty’s clinic. The exact origin of the Polydipsia/polyuria and mild hyperglycemia remains unclear but the history of long time topical cortone therapy might be involved.https://ijvst.um.ac.ir/article_26684_219ef4fc72aa92985679af7e18b8c0a0.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X4220121001Unusual case of cutaneous squamous cell carcinoma in an ewe49532641010.22067/veterinary.v4i2.16901ENVahid NajarnezhadDepartment of Clinical Sciences, School of Veterinary Medicine, University of Urmia, UrmiaMohammad Reza AslaniDepartment of Clinical Sciences, School of Veterinary Medicine, University of Sharekord, Sharekord0000-0001-5479-676XJournal Article20121113Squamous cell carcinoma (SCC) is a malignant tumor of epidermal cells in which the cells show differentiation to keratinocytes and is common in the horses, cattle, cats and dogs but is uncommon in the sheep. This communication describes a case of cutaneous squamous cell carcinoma (SCC) in a 5-year-old ewe. The tumor mass located on lumbar back and histological evaluation of biopsy sample revealed features of active SCC including atypical squamous cells with pleomorphism and hyperchromatism and atypical mitotic figures with dyskeratotic cells.https://ijvst.um.ac.ir/article_26410_d466e5a3b1881ebeeabaeded6dd512c8.pdf