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Behnam Roozbeh Mahtab Moazami Amir Rashidlamir Zahra Moosavi Ali Javadmanesh

Abstract

Growth hormone has mitotic and anti-apoptotic effects which may increase proliferation and transformation of cells when it is expressed aberrantly. This study investigated the effects of resistance training and growth hormone injection on circulating IGF-1, IGFBP-3 levels and IGF-1/IGFBP-3 ratio in male Wistar rats. Thirty-two male Wistar rats were randomly assigned to a control group (C, n = 8), a resistance training group (RT, n = 8), a growth hormone injection group (GI, n = 8) and a resistance training + growth hormone injection group (RG, n = 8). The resistance training protocol comprised of climbing a ladder (5 days/week, 3 sets/5 reps) while carrying a weight suspended from the tail. The growth hormone (2 mg/kg/day, 5 days/week) was injected before an exercise session. Serum IGF-1, IGFBP-3 levels, and IGF-1/IGFBP-3 ratio were measured after 8 weeks. One-way ANOVA analysis was used for comparison of serum IGF-1 and IGFBP-3 levels between groups. Serum IGF-1 levels and IGF-1/IGFBP-3 ratio signifi cantly decreased, but serum IGFBP-3 levels showed no significant change in the RT group compared to the C group. Also, both serum IGF-1 and IGFBP-3 levels and IGF-1/IGFBP-3 ratio in GI and RG groups significantly increased compared to the other groups. In conclusion, resistance training decreases serum IGF-1 levels and/or IGF-1/IGFBP-3 ratio in normal condition. On the other hand, the growth hormone injection with and without the resistance training increases serum IGF-1 levels and IGF-1/IGFBP-3 ratio which could be noted as a condition with a higher risk of neoplasm.

Article Details

Keywords

Resistance training, Growth hormone injection, IGF-1, IGFBP-3, Cancer

References
1. Bird SR, Goebel C, Burke LM, Greaves RF. Doping in sport
and exercise: anabolic, ergogenic, health and clinical issues.
Ann Clin Biochem. 2016;53(Pt 2):196-221.
2. Hermansen K, Bengtsen M, Kjaer M, Vestergaard P, Jorgensen
JOL. Impact of GH administration on athletic performance
in healthy young adults: A systematic review and
meta-analysis of placebo-controlled trials. Growth Horm IGF
Res. 2017;34:38-44.
3. Ghanipoor-Samami M, Javadmanesh A, Burns BM, Th omsen
DA, Nattrass GS, Estrella CAS, et al. Atlas of tissue- and
developmental stage specifi c gene expression for the bovine
insulin-like growth factor (IGF) system. PLoS One.
2018;13(7):e0200466.
4. Jones JI, Clemmons DR. Insulin-like growth factors and
their binding proteins: biological actions. Endocr Rev.
1995;16(1):3-34.
5. Denduluri SK, Idowu O, Wang Z, Liao Z, Yan Z, Mohammed
MK, et al. Insulin-like growth factor (IGF) signaling in tumorigenesis
and the development of cancer drug resistance.
Genes Dis. 2015;2(1):13-25.
6. Majorczyk M, Smolag D. Eff ect of physical activity on IGF-1
and IGFBP levels in the context of civilization diseases prevention.
Rocz Panstw Zakl Hig. 2016;67(2):105-11.
7. Allen NE, Key TJ. Re: plasma insulin-like growth factor-I, insulin-
like growth factor-binding proteins, and prostate cancer
risk: a prospective study. J Natl Cancer Inst. 2001;93(8):649-
51.
8. Chan JM, Stampfer MJ, Giovannucci E, Gann PH, Ma
J, Wilkinson P, et al. Plasma insulin-like growth factor-I
and prostate cancer risk: a prospective study. Science.
1998;279(5350):563-6.
9. Endogenous H, Breast Cancer Collaborative G, Key TJ, Appleby
PN, Reeves GK, Roddam AW. Insulin-like growth factor
1 (IGF1), IGF binding protein 3 (IGFBP3), and breast
cancer risk: pooled individual data analysis of 17 prospective
studies. Lancet Oncol. 2010;11(6):530-42.
10. Hankinson SE, Willett WC, Colditz GA, Hunter DJ, Michaud
DS, Deroo B, et al. Circulating concentrations of insulin-
like growth factor-I and risk of breast cancer. Lancet.
1998;351(9113):1393-6.
11. Singh P, Rubin N. Insulinlike growth factors and binding proteins
in colon cancer. Gastroenterology. 1993;105(4):1218-37.
12. Stattin P, Rinaldi S, Biessy C, Stenman UH, Hallmans G,
Kaaks R. High levels of circulating insulin-like growth factor-
I increase prostate cancer risk: a prospective study in
a population-based nonscreened cohort. J Clin Oncol.
2004;22(15):3104-12.
13. Johansson H, Baglietto L, Guerrieri-Gonzaga A, Bonanni B,
Mariette F, Macis D, et al. Factors associated with circulating
levels of insulin-like growth factor-I and insulin-like growth
factor binding protein-3 in 740 women at risk for breast cancer.
Breast Cancer Res Treat. 2004;88(1):63-73.
14. Yu H, Spitz MR, Mistry J, Gu J, Hong WK, Wu X. Plasma
levels of insulin-like growth factor-I and lung cancer risk: a
case-control analysis. J Natl Cancer Inst. 1999;91(2):151-6.
15. Zhao H, Grossman HB, Spitz MR, Lerner SP, Zhang K, Wu X.
Plasma levels of insulin-like growth factor-1 and binding protein-
3, and their association with bladder cancer risk. J Urol.
2003;169(2):714-7.
16. Ma J, Pollak MN, Giovannucci E, Chan JM, Tao Y, Hennekens
CH, et al. Prospective study of colorectal cancer risk in men
and plasma levels of insulin-like growth factor (IGF)-I and
18 IGF-binding protein-3. J Natl Cancer
Inst. 1999;91(7):620-5.
17. Renehan AG, O’Dwyer ST, Shalet SM.
RESPONSE: more about: prospective
study of colorectal cancer risk in
men and plasma levels of insulin-like
growth factor (IGF)-I and IGF-binding
protein-3. J Natl Cancer Inst.
2000;92(23):1949.
18. Koziris LP, Hickson RC, Chatterton
RT, Jr., Groseth RT, Christie JM,
Goldfl ies DG, et al. Serum levels of
total and free IGF-I and IGFBP-3 are
increased and maintained in longterm
training. J Appl Physiol (1985).
1999;86(4):1436-42.
19. Kraemer WJ, Aguilera BA, Terada M,
Newton RU, Lynch JM, Rosendaal G,
et al. Responses of IGF-I to endogenous
increases in growth hormone
aft er heavy-resistance exercise. J Appl
Physiol (1985). 1995;79(4):1310-5.
20. Kraemer WJ, Hakkinen K, Newton
RU, Nindl BC, Volek JS, McCormick
M, et al. Eff ects of heavy-resistance
training on hormonal response patterns
in younger vs. older men. J Appl
Physiol (1985). 1999;87(3):982-92.
21. Kraemer WJ, Marchitelli L, Gordon
SE, Harman E, Dziados JE, Mello R,
et al. Hormonal and growth factor
responses to heavy resistance exercise
protocols. J Appl Physiol (1985).
1990;69(4):1442-50.
22. Rosendal L, Langberg H, Flyvbjerg A,
Frystyk J, Orskov H, Kjaer M. Physical
capacity infl uences the response of insulin-
like growth factor and its binding
proteins to training. J Appl Physiol
(1985). 2002;93(5):1669-75.
23. Schwarz AJ, Brasel JA, Hintz RL, Mohan
S, Cooper DM. Acute eff ect of
brief low- and high-intensity exercise
on circulating insulin-like growth
factor (IGF) I, II, and IGF-binding
protein-3 and its proteolysis in young
healthy men. J Clin Endocrinol Metab.
1996;81(10):3492-7.
24. Eliakim A, Brasel JA, Mohan S,
Wong WL, Cooper DM. Increased
physical activity and the growth hormone-
IGF-I axis in adolescent males.
Am J Physiol. 1998;275(1 Pt 2):R308-
14.
25. Bang P, Brandt J, Degerblad M, Enberg
G, Kaijser L, Th oren M, et al. Exercise-
induced changes in insulin-like
growth factors and their low molecular
weight binding protein in healthy
subjects and patients with growth
hormone defi ciency. Eur J Clin Invest.
1990;20(3):285-92.
26. Mannerkorpi K, Landin-Wilhelmsen
K, Larsson A, Cider A, Arodell O,
Bjersing JL. Acute eff ects of physical
exercise on the serum insulin-like
growth factor system in women with
fi bromyalgia. BMC Musculoskelet
Disord. 2017;18(1):37.
27. Borst SE, De Hoyos DV, Garzarella
L, Vincent K, Pollock BH, Lowenthal
DT, et al. Eff ects of resistance training
on insulin-like growth factor-I and
IGF binding proteins. Med Sci Sports
Exerc. 2001;33(4):648-53.
28. Michael V. Vitiello, Charles W.
Wilkinson, George R. Merriam, Karen
E. Moe, Patricia N. Prinz, David
D. Ralph, et al. Successful 6-Month
Endurance Training Does Not Alter
Insulin-Like Growth Factor-I
in Healthy Older Men and Women.
Th e Journals of Gerontology.
1997;52A(3):M149-M54.
29. Redd MJ, Hoff man JR, Gepner Y,
Stout JR, Hoff man MW, Ben-Dov D,
et al. Th e eff ect of HMB ingestion on
the IGF-I and IGF binding protein
response to high intensity military
training. Growth Horm IGF Res.
2017;32:55-9.
30. Nishida Y, Matsubara T, Tobina T,
Shindo M, Tokuyama K, Tanaka K, et
al. Eff ect of low-intensity aerobic exercise
on insulin-like growth factor-I
and insulin-like growth factor-binding
proteins in healthy men. Int J Endocrinol.
2010;2010.
31. Chicharro JL, Lopez-Calderon A,
Hoyos J, Martin-Velasco AI, Villa G,
Villanua MA, et al. Eff ects of an endurance
cycling competition on resting
serum insulin-like growth factor
I (IGF-I) and its binding proteins IGFBP-
1 and IGFBP-3. Br J Sports Med.
2001;35(5):303-7.
32. Nindl BC, Pierce JR. Insulin-like
growth factor I as a biomarker of
health, fi tness, and training status.
Med Sci Sports Exerc. 2010;42(1):39-
49.
33. Eliakim A, Moromisato M, Moromisato
D, Brasel JA, Roberts C, Jr., Cooper
DM. Increase in muscle IGF-I protein
but not IGF-I mRNA aft er 5 days of
endurance training in young rats. Am
J Physiol. 1997;273(4 Pt 2):R1557-61.
34. Nishijima T, Piriz J, Dufl ot S, Fernandez
AM, Gaitan G, Gomez-Pinedo U,
et al. Neuronal activity drives localized
blood-brain-barrier transport of serum
insulin-like growth factor-I into
the CNS. Neuron. 2010;67(5):834-46.
35. Sachdev D, Yee D. Disrupting insulin-
like growth factor signaling as a
potential cancer therapy. Mol Cancer
Th er. 2007;6(1):1-12.
36. Collett-Solberg PF, Cohen P. Th e
role of the insulin-like growth factor
binding proteins and the IGFBP
proteases in modulating IGF action.
Endocrinol Metab Clin North Am.
1996;25(3):591-614.
37. Kemmler W, Wildt L, Engelke K, Pintag
R, Pavel M, Bracher B, et al. Acute
hormonal responses of a high impact
physical exercise session in early
postmenopausal women. Eur J Appl
Physiol. 2003;90(1-2):199-209.
38. Nindl BC, Kraemer WJ, Marx JO, Arciero
PJ, Dohi K, Kellogg MD, et al.
Overnight responses of the circulating
IGF-I system aft er acute, heavy-resistance
exercise. J Appl Physiol (1985).
2001;90(4):1319-26.
39. Sandhu MS, Dunger DB, Giovannucci
EL. Insulin, insulin-like growth
factor-I (IGF-I), IGF binding proteins,
their biologic interactions, and
colorectal cancer. J Natl Cancer Inst.
2002;94(13):972-80.
40. Lee S, Farrar PR. Resistance training
induces muscle-specifi c changes
in muscle mass and function in
Rat. Journal of Exercise Physiology.
2003;6(2).
How to Cite
Behnam, R., Mahtab, M., Amir, R., Zahra, M., & Ali, J. (2019). The Eff ect of resistance training and growth hormone injection on circulating IGF-1 and IGFBP-3 levels in a rat model. Iranian Journal of Veterinary Science and Technology, 11(1), 13-18. https://doi.org/10.22067/veterinary.v1i11.78504
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Original Articles