##plugins.themes.bootstrap3.article.main##

Seyed Amin Kazemi Asl Mohammad Reza Aslani Abdonaser Mohebbi Azam Mokhtari

Abstract

Contagious ecthyma (CE) is a zoonotic skin disease of small ruminants, caused by an epitheliotropic parapoxvirus and has a worldwide distribution with significant economic importance. The objective of this study was to determine clinicopathlogic abnormalities in goats naturally infected with CE. Thirty two goats, 16 affected with CE and 16 normal healthy goats were used in this study. CE was confirmed by histopathology and PCR. Blood samples were collected from jugular veins for hematological and biochemical analysis. The PCV, WBC and neutrophil counts of CE affected goats were significantly higher than those in the unaffected goats (p < 0.05). Serum biochemical analysis revealed significantly higher levels of BUN, glucose, MDA and iron concentrations as well as CK, AST, GGT and catalase activities in CE affected goats than healthy animals (p < 0.05). The serum activity of catalase, SOD and GPx in goats with CE were significantly lower than those in normal goats. Creatinine concentration in serum of goats with CE was significantly lower than that in heathy ones (p < 0.05). There was no significant difference in serum total protein, albumin, total and direct bilirubin, and cholesterol concentrations between CE affected and healthy goats. The alterations observed in hematological and biochemical parameters of CE affected goats could be related to weight loss, subnutrition, oxidative stress and pathological changes including inflammation and secondary bacterial infection. These findings could be useful for the management of cases of sheep and goats with CE.

Article Details

Keywords

Contagious ecthyma, Goats, Parapoxvirus, Orf, Clinical pathogy

References
1. Constable PD, Hinchcliff KW, Done SH, Grumberg W. Veterinary
Medicine, A text Book of the Diseases of Cattle, Horses,
sheep, Pigs and goats. 11th edition, 2017; Elsevier, PP. 1593-
1596.
2. Reid HW, Rodger SM. Orf. In: Diseases of sheep. 4th edition,
2007;Blakwell, Ames-Iowa, PP. 297-302.
3. Spyrou V, Valiakos G. Orf virus infection in sheep or goats. Vet
Microbiol. 2015;181: 178-182.
4. Hasheminasab SS, Mahmoodi A, Mahmoodi P, Maghsood H.
Orf virus infection in human ecthymacontagiosum: a report
of two cases in the West of Iran. Virus Dis. 2016; 27: 209-210.
5. Abba Y, Igbokwe IO, Adamu L, Buba I. Alterations in hematological
and serum biochemical parameters of Sahel goats with
clinical mastitis. IOSR J Agr Vet Sci. 2013;7: 74-77.
6. Hashemnia M, Khodakaram-Tafti A, Rasavi SM, NasifiS.Hematological
and serum biochemical analyses in experimental
caprine coccidiosis. J Parasit Dis. 2014;38:116–123.
7. Kumar A, VihanVS, Sharma HN. Haematological and biochemical
effects of tick infestation in common Indian goat.
Advance Biores. 2010;1: 163-168.
8. Neamat-Allah ANF. Immunological, hematological, biochemical,
and histopathological studies on cows naturally infected
with lumpy skin disease. Vet World, 2015; 8: 1131-1136.
9. Sevik M, Avci O, Dogan M, Ince OB. Serum biochemistry of
lumpy skin disease virus- infected cattle. Biomed Res Inter.,
2016; doi.org/10.1155/2016/6257984.
10. Kaneko JJ, Harvey JW, Bruss ML. Clinical Biochemistry of
Domestic Animals. 2008; 6th edition, Academic Press,
11. Koner BC, Banerjee BD, Ray A. Effects of stress on gamma
glutamyltranspeptidase (GGT) activity in lymphoid system of
rats: modulation by drugs. Indian J Exp Biol. 1997:35:222-4.
12. Geogieva NV. Oxidative stress as a factor of disrupted ecological
oxidative balance in biological systems – a review. Bulg J
Vet Sci. 2005; 8:1-11.
13. Celi P. The role of oxidative stress in small ruminants’ health
and production. R BrasZootec. 2010;39: 348-363.
14. Celi P, Gabai G. Oxidant/antioxidant balance inanimal nutrition
and health: the roleof protein oxidation. Frontiers in
VetSci. 2015; 2: 1-13.
15. Kirmizigul AH., Orgun M., Ozen H., Erkilic EE., Gokce E.,
Karman M., Kukurt A. Oxidative stress and total sialic acid
levels in sheep naturally infected with pox virus. Pak Vet J.
2016;36: 312-315.
16. Kizil O., Ozdemir H., Karahan M., Kizil M. Oxidative stress and
alterations of antioxidant status in goats naturally infected with
mycoplasma agalactiae. Revue MédVét. 2007; 158:326-330.
17. Valyi-Nagy T, Olson SJ, Valyi-Nagy K, Montine TJ, DermodyTS.
Herpes simplex virus type 1 latency in the murine nervous
system is associated with oxidative damage to neurons.
Virol. 2000; 278: 309-321.
18. Schachtele SJ, Hu S, Little MR, Lokensgard JR. Research Herpes
simplex virus induces neural oxidative damage via microglial
cell Toll-like receptor-2. JNeuroinflammation. 2010; 7:35.
19. Tawadrous GA, Aziz AA, Amin DG, Eldemery A, Mostafa
MAA. RANTES, TNF-alpha, oxidative stress, and hematologicalabnormalities
in hepatitis C virus infection. J Invest Med.
2012; 60:878–882.
20. Phanka M. Role of oxidative stress in infectious diseases: a
review. Folia Microbiol. 2013; 58:503–513.
21. Goth L. A simple method for determination of serum catalase
activity and revision of reference range. ClinChimActa. 1991;
196:143–151.
22. Sun YI, Oberley LW, Li Y. A simple method for clinical assay
of superoxide dismutase. ClinChem 1988; 34: 497-500.
23. Paglia DE, Valentine WN. Studies on the quantitative and qualitative
characterization of erythrocyte glutathione peroxidase.
J Lab ClinMed 1967; 70: 158-169
24. Buege JA and Aust SD. Microsomal lipid peroxidation. Meth
Enzymol. 1978; 52: 302.
How to Cite
Seyed Amin, K. A., Mohammad Reza, A., Abdonaser, M., & Azam, M. (2019). Hematological and biochemical evaluation of goats naturally infected with contagious ecthyma. Iranian Journal of Veterinary Science and Technology, 10(2), 43-47. https://doi.org/10.22067/veterinary.v2i10.69865
Section
Original Articles