Detection of Ciprofloxacin resistance genes in Escherichia coli isolated from dogs with urinary tract infections

Document Type : Research Articles

Authors

• Muhammad Mustapha ¹
• Parveen Goel ²

¹ Department of Veterinary Medicine, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana India.

² Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana India.

10.22067/veterinary.v12i1.86316

Abstract

This research was performed on uropathogenic Escherichia coli (E. coli) isolates and established the genes of resistance to ciprofloxacin between the isolates. A total of one hundred and three urine samples were tested for uropathogenic E. coli which were obtained from dogs with urinary tract infections (UTIs) using cultural isolation, antimicrobial susceptibility test, and polymerase chain reaction (PCR). The results revealed that genes associated with ciprofloxacin resistance are 24.3% positive for E. coli. The E. coli isolates were resistant to both ciprofloxacin and ampicillin (100%), highly susceptible to chloramphenicol (84.0%), and less susceptible to gentamycin (44.0%) and amikacin (40.0%). The PCR tests showed the presence of the ParC (in 25 samples; 100%), GyrA (in 25 samples; 100%), and GyrB (in 4 samples; 16.0%) genes. The findings of the present study showed an upsetting rate of ciprofloxacin and ampicillin resistance among the E. coli isolates from dogs with UTIs.

Keywords

• Dog
• Uropathogenic E. coli
• Antibiotic-resistant genes
• Ciprofloxacin
• PCR

20.1001.1.2008465.2020.12.1.3.9

Main Subjects

• Pharmacology

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