Effect of Zataria multiflora essential oil on rooster semen during storage at 4°C

Document Type : Research Articles

Authors

• Fahimeh Geraminezhad ¹
• Mohammad Roostaei-Ali Mehr ²

¹ University of Guilan

² Unversity of Guilan

10.22067/veterinary.v10i1.72126

Abstract

Experiment was conducted to determine effect of Zataria multiflora boiss essential oil on stored spermatozoa. Semen collection was performed by using 15 mature roosters twice a week at four times. In each session, ejaculates were pooled and split into seven parts. The amounts of 0 (EO0), 50 (EO50), 100 (EO100), 200 (EO200), 400 (EO400), 600 (EO600) and 1000 (EO1000) ng/ml Zataria multiflora boiss essential oil were added to each part. Samples were chilled to 4°C and maintained for 72 h. Sperm assessment was performed at 0, 24, 48 and 72 h. Lipid peroxidation was evaluated after 48 h. Results showed that there was no interaction between Zataria multiflora essential oil and incubation time on membrane integrity, sperm motility and viability (p > 0.05). The highest sperm progressive motility (80.43%), viability (86.31%) and functional membrane integrity (85.81%) was observed in EO200 (p < 0.05). The lowest sperm motility (61.31%) and viability (73.31%) was observed in EO1000 (p < 0.05). The concentrations of malondialdehyde was lowest in EO200 (0.17 nM/ml, p < 0.05). Therefore, addition of 200 ng/ml Zataria multiflora boiss essential oil to semen improved longevity of rooster spermatozoa at 4°C.

Keywords

• Antioxidant
• Essential oil
• rooster sperm

20.1001.1.2008465.2018.10.1.3.5

Main Subjects

• Food hygiene
• Poultry disease

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During the time of storage, membrane integrity, sperm viability and motility were declined, which was in agreement with study performed by other investigators [20]. Decrease in quality of stored spermatozoa in liquid form related to the accumulation of the toxic products of metabolism [21]

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