Preparation and in vitro evaluation of chitosan-based films for the sustained delivery of enrofloxacin

Document Type : Research Articles

Authors

• Ali Rassouli ¹
• Sakineh Khanamani Falahatipour ²
• Yalda Hosseinzadeh Ardakani ³
• Hamid Akbari Javar ³
• Katayoun Kiani ¹
• Taghi Zahraee Salehi ¹

¹ University of Tehran

² Rafsanjan Univ Med Sci

³ Tehran University of Medical Sciences

10.22067/veterinary.v10i1.70288

Abstract

The implantable drug products are developed mainly to sustain the drug release. This study was conducted to formulate and evaluate cross-linked films of chitosan/β-glycerophosphate (β-GP) for the sustained delivery of enrofloxacin (ENR). Two types of formulations, single-layer (F1 and F2) and triple-layer (F3 and F4) films, were prepared. In vitro drug release, kinetic modelling, Fourier transform infrared spectroscopy (FTIR) spectra, morphological and microbiological studies were performed. Drug release from F1 and F2 continued up to 5 hours but from F3 and F4, it was extended over 96 and 168 hours, respectively. The cumulative drug release for F1, F2, F3 and F4 were 72.6, 70.1, 90.5 and 82.4%, respectively. The inhibition zones of bacterial growth by using positive controls and single layer films were significantly greater than those of triple-layer films (p < 0.05), indicating sustained drug release pattern of the multi-layer films. These findings suggest that the triple-layer chitosan/β-GP films could be effective to deliver ENR for a long period.

Keywords

• Enrofloxacin
• Chitosan
• β-glycerophosphate
• Sustained release
• Triple-layer film

20.1001.1.2008465.2018.10.1.2.4

Main Subjects

• Bacteriology
• Infectious diseases
• Pharmacology

---

References

Rajgor N, Patel M and Bhaskar VH. Implantable drug delivery systems: An overview. Syst. Rev. Pharm. 2011; 2: 91-95.

Iqbal MM, Gupta S, Sagar S and Ibrahim M. Design and evaluation of subcutaneous implantable drug delivery system of tramadol using natural biodegradable polymer. Ann. Phytomed. 2012; 1: 30-8.

Hoare TR and Kohane DS. Hydrogels in drug delivery: progress and challenges. Polymer. 2008; 49(8): 1993-2007.

Pandya Y, Sisodiya D and Dashora K. Atrigel, implants and controlled released drug delivery system. Int. J. Biopharmaceut. (2014) 5(3): 208-213.

Begin A and Van Calsteren MR. Antimicrobial films produced from chitosan. Intl. J. Biol. Macromol. 1999; 26(1): 63-67.

Li C, Ren S, Dai Y, Tian F, Wang X, Zhou S, Deng S, Liu Q, Zhao J and Chen X. Efficacy, pharmacokinetics, and biodistribution of thermosensitive chitosan/β-glycerophosphate hydrogel loaded with docetaxel. AAPS Pharm. Sci. Tech. 2014; 15(2): 417-424.

Shuwisitkul D. Biodegradable implants with different drug release profiles. [Dissertation]. Freie Universität Berlin, Germany. 2011; 1-46.

Martinez M, McDermott P and Walker R. Pharmacology of the fluoroquinolones: a perspective for the use in domestic animals. The Vet. J. 2006; 172(1): 10-28.

Rao CHR and Arunkumar C. Development of a new validated HPLC method for analysis of antibiotics in aqua food chloramphenicol, enrofloxacin in shrimps. Int. J. A. PS. BMS. 2012; 1(4): 398-412.

Radha CV, Swetha N, Bharathi P, Aruna Gowri PSSRK and Neeraja N. Formulation and evaluation of transdermal films of enalapril maleate. J. Pharmaeut. Sci. Innov. 2013; 2(1): 57-60.

Kyo-Han KIM and Ramaswamy N. Electrochemical surface modification of titanium in dentistry. Dental Materials Journal. 2009; 28(1): 20-36.

Jiang B, Liang J, Sun C, Lan J, Sun X and Huang H. Preparation and characterization of chitosan/β-GP membranes for guided bone regeneration. Journal of Wuhan University of Technology-Mater. Sci. Ed. 2011; 26(2): 241-245.

Shivashankar M and Mandal, BK. Formulation and evaluation of bupivacaine-loaded glutaraldehyde-crosslinked high molecular weight chitosan microspheres. Trop. J. Pharmaceut. Res. 2013; 12(1): 13-18.

Tabandeh H, Mortazavi SA and Guilani TB. Preparation of sustained-release matrix tablets of aspirin with ethylcellulose, Eudragit RS100 and Eudragit S100 and studying the release profiles and their sensitivity to tablet hardness. Iranian J Pharmaceut Res. 2010; 201-206.

Dhanikula AB and Panchagnula, R. Development and characterization of biodegradable chitosan films for local delivery of paclitaxel. The AAPS Journal. 2004; 6(3): 88-99.

Aviv M, Berdicevsky I and Zilberman M. Gentamicin‐loaded bioresorbable films for prevention of bacterial infections associated with orthopedic implants. J. Biomed. Materials Res. Part A. 2007; 83(1): 10-19.

Mashak A, Mobedi H and Mahdavi H. A Comparative study of progesterone and lidocaine hydrochloride release from poly (L-lactide) films. Pharmaceut. Sci. 2015; 21(2): 77-85.

Kalam MA, Humayun M, Parvez N, Yadav S. Garg A, Amin S. Sultana Y and Ali A. Release kinetics of modified pharmaceutical dosage forms: A Review. Continental J. Pharmaceut. Sci. 2007; 1:30–35.

Jahangirian MJ, Haron H, Shah MH, Abdollahi Y, Rezayi M and Vafaei N. Well diffusion method for evaluation of antibacterial activity of copper phenyl fatty hydroxamate synthesized from canola and palm kernel oils. Digest Journal of Nanomaterials & Biostructures. 2013; 8(3): 1263-1270.