Effects of different antimicrobials agents on mycoplasma species isolated from ruminants by macro culture technique

Document Type : Research Articles

Author

• Roya Sadri

Razi vaccine & serum research Institute

10.22067/veterinary.v4i1.26468

Abstract

Mycoplasma is an important respiratory tract pathogen worldwide, causing respiratory tract infection in small ruminants ( sheep and goats). It is a bacterium that causes acute respiratory illness ranging in severity from mild respiratory illness to severe pneumonia. Mycoplasmosis in small ruminants is a serious and major problem in Iran. This study was determined to isolate Mycoplasma species and detect antibacterial effect of Oxytetracycline, Tylosine, Chloramphenicole, Erythromycine, Enrofloxacin and Florfenocole on respiratory Mycoplasma subsp in small ruminantes by Macro culture technique. As, there is no an effective vaccine against Mycoplasma disease, treatment and controlling is mainly by chemotherapy. Basically, it was approved that uncontrolled usage of antimicrobial elements has caused the development of antimicrobial resistance. The antimicrobial susceptibility test showe some Mycoplsma species –specific differences, with M.capricolum subsp. It was more susceptible to erythromycine and Tylosine, while Florfeniclole and Chloramphenocole were the least effective for all three Mycoplasma species. It is observed that there was not any significant difference in antimicrobial susceptibility between goat and sheep isolates or between isolate from different regions in affected province. Results showed that some isolates of M.capricolum.and M. putrefaciens had minimal inhibitory concentration (MIC) level with Oxytetracyclin as was the same with two isolates of M.mycoides subsp. mycoides LC with Tylosine. It seems resistance factor against antimicrobials is involved.

Keywords

• susceptibility
• antimicrobial
• Mycoplasma
• small ruminants
• Macro culture
• MIC

20.1001.1.2008465.2012.4.1.8.8

Main Subjects

• Bacteriology
• Ruminator

---

References

Aaraabi, I. Sotoodehnia , A. (1984) Isolation and carbonydrate fermentation tests of Mycoplasma agalactiae and Mycoplasma subsp, Mycoides strains in Iran . Archives of Razi Institute 34-

35,67-70.

Ayling , R.D. , Baker, S.E., Nicholas, R.A.J.,Peek, M.L. and Simon, A. J. (2000)Comparison of in vitro activity of danofloxacin, florfenicol,oxytetracycline, spctinomycin and

tilmicosin against Mycoplasma mycoides subsp. mycoides small

colony type .Veterinary Record 146,243-245.

Bashiruddin. J. B., Taylor, T.K. and Gould, A.R. (1994) A PCR based test for the specific identification of M. mycoides

sub species mycoides SC. Journal of Veterinary Diagnostics and

Investigation 6, 428-434.

Bebear, C. (1996) Introductory remarks.In:J.G.Tully and S.Razin (eds),Molecular and Diagnostic procedures,vol.II, Academic press,London 181-183.

Ghaleh, G.B.N. (2006) Prevalence determination of agalactia in Fars province and gentic identification of mycoplsma agent producing disease by PCR. Project final report, Razi Vaccine and Serum Research Institute.Hannan, P.C.T. (2000) Guidelines and

recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary Mycoplasma species .Veterinary Research 31, 373-395.

Loria, G. P., Sammartino,C. , Nicolas, and Ayling, R. D. (2003) In

vitrosusceptibilities of field isolates of Mycoplasma agalactia to

oxytetracyclin and tylosin,enrofloxacin, spiramycin and lincomycin.-spectinimycine . Research in Veterinary Science 75, 3-7.

Nicolas, R., Bashiruddin, J., Ayling, R.and Miles, R.J. (2000) Contagious bovine pleuropneumonia ; a review of recent

developments. Veterinary Bulletin 70 (8), 827-838.

Nicholas, R., Ayling, R., McAuliffe, L. (2008)Mycoplasma Diseases of Ruminants,Centre for Agricultural Bioscience International, 199- 200.

Proveda, J.B. and Nicholas, R. A. J. (1998)Serological identification by growth inhibition and metabolic inhibition

tests. In: R. J. Miles and R. A. J.Nicolas (eds), Mycoplasma protocols,Humana Press , 105-113.

Puglisi, J.D., Blanchar D.C., Dahlquist, K.D.,Eason, R.G., Fourmy, D., Lynch, S.R.,Recht,M.I. and Yoshizawa, S. (2000) Aminoglycoside antibiotics and decoding. In: R.A.Carret,

S.R.Douthwaite , A .Liljas ,A.T.Matheson ,P.B. Moore and H. F. Noller (eds) , The Ribosomes structure Functions,Antibiotics and Cellular Interactions. American Society for Microbiology ,Washington DC, 419-430.

Woods,G.L. and Washington, J.A. (1995)Antibacterial susceptibility tests,dilution and disk diffusion methods.In: Manual of Clinical Microbiology,American Society for Microbilogy,Washington DC, 1327-1341.