Behrooz Fathi Fatemh Amani Atena Jami al ahmadi Abbase Zare


Echis carinatus (Saw-scale viper) is one of the most venomous snakes in Asia and some
parts of the Africa with deadly hemotoxic venom. It has been reported that suramin, an antitrypansomiasis
drug, can inhibits the toxic effects of some snake venoms. This study was
conducted to evaluate the antagonistic effects of suramin against the venom of the Iranian
snake Echis carinatus.
Adult albino mice weighing 35±5g were divided into nine groups (A1, A2, B1, B2, B3, C1,
C2, D and E) of 6-10 mice. Groups A1 and A2 (protocol A), which received the venom at 7 and
13 mg/kg, succumbed after 169±32 min and 53±16 min, respectively. Groups B1, B2 and B3
(protocol B) were treated with different doses of suramin 15 min after injection of venom.
The results show that suramin significantly delayed time to death in groups B1, B2 and B3
compared with groups A1 and A2 (P<0.05). In groups C1 and C2 (Protocol C), venom was preincubated
with suramin for 15 min prior to injection into animals. The survival times of these
two groups were significantly increased when compared to group A2. Hhowever, only group
C1 in comparison with group A1 had a significantly increased survival time (P<0.05). Group D
(protocol D) was treated with suramin 15 min before injection of venom. Time to death in this
group increased in comparison to group A2. Group E received suramin alone as a control, and
all the animals in this group remained alive. The results of this study showed that a single
dose of suramin have a protective role against Echis carinatus venom at least in delaying time
to death of envenomed animals. Repeated administration or higher doses of suramin may be
able to prevent death caused by the venom

Article Details

Ali, G., Kak, M., Kumar, M., Bali, S.K., Tak,S.I., Hassan, G. and M.B., W. (2004)Acute renal failure following echis
carinatus (saw-scaled viper) envenomation.Indian Journal of Nephrology 14, 177-181.
Arruda, E.Z., Silva, N.M., Moraes, R.A. and Melo, A.P. (2002) Effect of suramin on myotoxicity of some crotalidsnake venom.
Brazilian Journal of Medical and Biological Research 35, 723-726.
Backshall, S. (2007) Steve Backshall's venom: Poisonous creatures in the natural world,New Holland publishers (UK) Ltd,
Bawaskar, H.S. and Bawaslar, P.H. (2002) Aphasia in a farmer after viper bite. Lancet 30, 23-25.
Emam, S.J. and Nikzamir, A. (2008) Evaluation of haematological and biochemical parameters in snakebite patients referred to razi hospital, Ahwaz,Iran. Pakistan Journal of Medical Sciences
25, 712-718.
Fathi, B. (2001) The effect of snake venom phospholipase A2 on cloned voltage-gated potassium channels and on neuromuscular
function, University of Strathclyde, Glasgow.
Fernandes, R.S., Assafim, M., Arruda, E.Z.,Schwartz, E.F., Melo, P.A., Zingali, R.B.and Monterio, R.Q. (2007) Suramin counteracts the haemostatic disturbances produced by Bothrops jararaca snake venom. Toxicon 49, 931-938.
Henning, R., Nelemans, H.A., Scaf, A.H.J., Eekeren, J.V., Agoston, S. and Hertog, A.D. (1992) Suramin reverses nondepolarizing neuromuscullar blockade in rat diaphragm. European Journal of Pharmacology 216, 73-79.
Kemparaj, K.B., Prasad, N. and Gowda, V.T.(1994) Purification of a basic phospholipase A2 from Indian saw-scaled viper (Echis carinatus) venom:characterization of antigenic, catalytic and
pharmacological properties. Toxicon 32,1187-1196.
Lin-Shiau, S.Y. and Lin, M.J. (1999) Suramin inhibits the toxic effects of presynaptic neurotoxins at the mouse nerve terminals.
European Journal of Pharmacology 382,75-80.
Mallow, D., Ludwig, D. and Nilson, G. (2003) True Vipers: Natural History and Toxinology of Old World Vipers, Krieger Publishing Company, Malabar, Florida.
Marsh, N.A. (1994) Snake venom affecting haemostatic mechanism- a consideration of their mechanism, practical applications and biological significance. Blood Coagulation
and Fibrinolysis 5, 399-410.
Monterio, R.Q., Campana, P.T., Melo, P.A.and M.L., B. (2004) Suramin interaction with human alpha-thrombin: inhibitory
effects and binding studies. Int. J.Biochem. Cell Biol 36, 2077-2085.
Oliveira, D.M., Cavalcante, W.L., Arruda,E.Z., Melo, P.A., Dal-Pai Silva, M. and Gallacci, M. (2003) Antagonism of myotoxic and paralysis activities of bothropstoxin-I by suramin. Toxicon 42,
Phelps, T. (1981) Poisonous Snakes, Blandford Press, Dorset, UK.
Sifuentes, D.N., El-Kik, C.Z., Ricardo, H.D., Tomaz, M.A., Strauch, M.A., Calil-Elias, S., Arruda, E.Z., Schwartz, E.F. and Melo, P.A. (2008) Ability of suramin to antagonize the cardiotoxic activities of Bothrops jararacussu venom. Toxicon 52,
Stein, C.A. (1993) Suramin, a novel antineoplastic agent with multiple potential mechanisms of action. Cancer Research
53, 2239-2248.
Warrell, D.A., Davidson, N.M., Greenwood, B.M., Ormerod, E.D., Pope, H.M., Watteins, B.J. and Prentice, C. (1977) Poisoning by Bites of the Saw-Scaled or Carpet Viper (Echis carinatus) in Nigeria.QJM: An International Journal of
Medicine 46, 33-62.
How to Cite
FathiB., AmaniF., Jami al ahmadiA., & ZareA. (2010). Antagonistic Effects of Suramin Against the Venom of the Iranian Snake Echis carinatus in Mice. Iranian Journal of Veterinary Science and Technology, 2(1), 9-16. https://doi.org/10.22067/veterinary.v2i1.3943
Original Articles