The Fabrication of a Biomedical Scaffold from Human Placenta

Document Type : Short communication

Authors

• Jassim mohammed Khalaf Albozachri ¹
• Hameed Ali AL-Timmemi ²

¹ Department of Veterinary Surgery and Obstetrics, College of Veterinary Medicine, University of Kerbala,Kerbala, Iraq.

² Department of Veterinary Surgery and Obstetrics, Collegeof Veterinary Medicine, University of Baghdad, Baghdad, Iraq.

10.22067/ijvst.2024.88051.1376

Abstract

The powder derived from human placenta (HP) was successfully used in regenerative medicine. The purpose of this study was to fabricate powder from the human placenta and evaluate it by histological analysis, scanning electron microscopy, and X-ray diffraction. The placenta was decellularized chemically and then lyophilized by a lyophilizer (FTS Systems Bulk Freeze Dryer Model 8–54) for 24 hours at -56 °C and 5 mm Hg until they were totally dried. The assessment used histological analysis, Scanning Electron Microscopy, and x-ray diffraction. The hematoxylin and eosin stain demonstrated that cellular populations and nuclear residues were totally absent from HP tissue. The freeze-drying process of preparing acellular human placenta powder resulted in structures that are made up of highly interconnected, open networks of pores. The particle size mean diameter was approximately ranging from a minimum of 89.44 µm to a maximum of 172.82 µm, and the pore sizes ranged between 44.28 µm and 81.40 µm. Using conventional diffraction database cards, the X-ray diffraction analysis of acellular human placenta powder demonstrated the existence of the constituent organic and inorganic components. It was discovered that the presence of semi-crystalline or amorphous organic components, such as chondroitin sulfate, collagen, and hyaluronic acid. The study concluded from the structural powder that it can be used in regeneration treatments such as treating the spinal cord in animals.

Keywords

• powder
• X-Ray Diffraction
• Extracellular matrix
• Scanning Electron Microscopy
• histological analysis

Main Subjects

• Histology

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