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Muhammad Mustapha Parveen Goel

Abstract

This research was performed on uropathogenic Escherichia coli (E. coli) isolates and established the genes of resistance to ciprofloxacin between the isolates. A total of one hundred and three urine samples were tested for uropathogenic E. coli which were obtained from dogs with urinary tract infections (UTIs) using cultural isolation, antimicrobial susceptibility test, and polymerase chain reaction (PCR). The results revealed that genes associated with ciprofloxacin resistance are 24.3% positive for E. coli. The E. coli isolates were resistant to both ciprofloxacin and ampicillin (100%), highly susceptible to chloramphenicol (84.0%), and less susceptible to gentamycin (44.0%) and amikacin (40.0%). The PCR tests showed the presence of the ParC (in 25 samples; 100%), GyrA (in 25 samples; 100%), and GyrB (in 4 samples; 16.0%) genes. The findings of the present study showed an upsetting rate of ciprofloxacin and ampicillin resistance among the E. coli isolates from dogs with UTIs.

Article Details

Keywords

Dog, Uropathogenic E. coli, Antibiotic-resistant genes, Ciprofloxacin, PCR

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How to Cite
MustaphaM., & GoelP. (2020). Detection of Ciprofloxacin resistance genes in Escherichia coli isolated from dogs with urinary tract infections. Iranian Journal of Veterinary Science and Technology, 12(1), 21-28. https://doi.org/10.22067/veterinary.v12i1.86316
Section
Original Articles