Modulation of growth performance, haemato-immunological parameters, gut microbiota and stress resistance upon feeding juvenile Schizothorax zarudnyi (Nikolskii, 1897) by fructo-oligosaccharid

Document Type : Research Articles

Authors

1 Islamic Azad University, Kashmar Branch, Kashmar, Iran

2 Ferdowsi University of Mashhad

Abstract

A 63-day experiment was carried out under controlled conditions to compare the effects of fructo-oligosaccharide (FOS) at four levels (5, 10, 20 and 30 g/kg) on growth performance, nutritional efficiency indices, haemato-immunological parameters, stress resistance, digestive enzymes and cultivable autochthonous intestinal microbiota of juvenile (68.52 ± 1.52 g) Khaju fish Schizothorax zarudnyi. Fish fed the diet containing 20 g/ kg FOS had significantly (p < 0.05) higher weight. Dietary FOS supplementation (5-20 g/kg) showed significant effects on SGR compared with control treatment. Hb, Haematocrit, MCV, MCH and lymphocytes in fish fed with the diet containing 20-30 g/kg FOS were significantly higher than those in fish fed with control treatment. After 63-day feed ing period and also, 5-min air exposure challenge test, the activities of IG, LYZ and ACP in serum of fish fed with the diet containing 10-30 g/kg FOS showed a significantly higher trend than other treatments. The ratio of lactobacillus count to total autochthonous intestinal microbiota in fish fed with 10-30 g/kg FOS was significantly higher than that in other treatment groups. Furthermore, dietary FOS supplementation significantly increased survival rate of juvenile Khaju fish. Polynomial regression of SGR, FCR, PPV and PER suggested that the optimum dietary FOS level could be higher than 18.2 and < 23.8 g/kg in fish reared in culture conditions. These results indicate the beneficial effects of FOS, and emphasizes the need for further research to analyze the use of prebiotics on growth performance of fish.

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Main Subjects


1. Coad BW. Systematic biodiversity in the freshwater fishes of Iran. Ital J Zool. 1998; 65 (sup1): 101–8.
2. Keykha S, Gharaei A, Mirdar Harijani J, Ghaffari M, Rahdari A. Antifungal effects of metalonic sumac (Rhus coriaria L.) essential oil on Schizothorax zarudnyi eggs. Journal of Veterinary Research. 2015; 70 (2), 131-137.
3. Rahdari A, Gharaei A, Ghaffari M. Spawning latency period in hormonal induced reproduction of snow trout (Schizothorax Zarudnyi (Nikolskii, 1897)). Iran J Biotechnol. 2014;12(1):1–5.
4. Glencross BD, Booth M, Allan GL. A feed is only as good as its ingredients - A review of ingredient evaluation strategies for aquaculture feeds. Aquac Nutr. 2007;13(1):17–34.
5. Raubenheimer D, Simpson S, Sanchez-Vazquez J, Huntingford F, Kadri S, Jobling M. Nutrition and Diet Choice. In: Aquaculture and Behavior. Wiley-Blackwell; 2012. p. 150–82.
6. Safari O, Shahsavani D, Paolucci M, Mehraban Sang Atash M. Screening of selected feedstuffs by sub-adult narrow clawed crayfish, Astacus leptodactylus leptodactylus Eschscholtz, 1823. Aquaculture. 2014;420–421.
7. Safari O, Shahsavani D, Paolucci M, Atash MMS. Single or combined effects of fructo- and mannan oligosaccharide supplements on the growth performance, nutrient digestibility, immune responses and stress resistance of juvenile narrow clawed crayfish, Astacus leptodactylus leptodactylus Eschscholtz, 182. Aquaculture. 2014;432:192–203.
8. Molina-Poveda C. 4 - Nutrient requirements A2 - Nates, Sergio F. BT - Aquafeed Formulation. In San Diego: Academic Press; 2016. p. 75–216.
9. Trichet VV. Nutrition and immunity: An update. Aquac Res. 2010;41(3):356–72.
10. Hoseinifar SH, Soleimani N, Ringø E. Effects of dietary fructo-oligosaccharide supplementation on the growth performance, haemato-immunological parameters, gut microbiota and stress resistance of common carp (Cyprinus carpio) fry. Br J Nutr [Internet]. 2014 Oct 3 [cited 2017 May 24];112(8):1296–302. Available from: http:// www.ncbi.nlm.nih.gov/pubmed/25313574
11. Mahious AS, Gatesoupe FJ, Hervi M, Metailler R, Ollevier F. Effect of dietary inulin and oligosaccharides as prebiotics for weaning turbot, Psetta maxima (Linnaeus, C. 1758). Aquac Int. 2006;14(3):219–29.
12. Van Loo J, Cummings J, Delzenne N, Englyst H, Franck A, Hopkins M, et al. Functional food properties of non-digestible oligosaccharides: a consensus report from the ENDO project (DGXII AIRII-CT94-1095). Br J Nutr.1999 Feb;81(2):121–32.
13. Lee C-S, Lim C, Gatlin DM, Webster CD, editors. Dietary Nutrients, Additives, and Fish Health. Hoboken, NJ, USA: John Wiley & Sons, Inc; 2015.
14. Merrifield DL, Dimitroglou A, Foey A, Davies SJ, Baker RTMM, Bøgwald J, et al. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture. 2010 Apr;302(1– 2):1–18.
15. Ringø E, Olsen Ree, Gifstad TØ, Dalmo RAA, Amlund H, Hemre G-II, et al. Prebiotics in aquaculture: a review. Aquac Nutr. 2010 Apr;16(2):117–36.
16. Anguiano M, Pohlenz C, Buentello A, Gatlin DM. The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum (Sciaenops ocellatus) and hybrid striped bass (Morone chrysops × M. saxatilis). Br J Nutr. 2012;1–7.
17. Zhou Q-C, Buentello JA, Gatlin DM. Effects of dietary prebiotics on growth performance, immune response and intestinal morphology of red drum (Sciaenops ocellatus). Aquaculture. 2010 Nov;309(1– 4):253–7.
18. Soleimani N, Hoseinifar SH, Merrifield DL, Barati M, Abadi ZH. Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol. 2012;32(2):316–21.
19. Akrami R, Iri Y, Khoshbavar Rostami H, Razeghi Mansour M. Effect of dietary supplementation of fructooligosaccharide (FOS) on growth performance, survival, lactobacillus bacterial population and hemato-immunological parameters of stellate sturgeon (Acipenser stellatus) juvenile. Fish Shellfish Immunol. 2013;35(4):1235–9.
20. Wu Y, Liu WB, Li HY, Xu WN, He JX, Li XF, et al. Effects of dietary supplementation of fructooligosaccharide on growth performance, body composition, intestinal enzymes activities and histology of blunt snout bream (Megalobrama amblycephala) fingerlings. Aquac Nutr. 2013;19(6):886–94.
21. Hoseinifar SH, Mirvaghefi A, Merrifield DL, Amiri BM, Yelghi S, Bastami KD. The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose. Fish Physiol Biochem. 2011;37(1):91–6.
22. Buentello JA, Neill WH, Gatlin, DM. Effects of dietary prebiotics on the growth, feed efficiency and non-specific immunity of juvenile red drum Sciaenops ocellatus fed soybean-based diets. Aquac Res. 2010;41(3):411–8.
23. Jahangoo V, Gharaei A, Akrami R. Effect of prebiotic mannanoligosaccharide on growth performance, survival and body composition of Schizothorax zarudnyi. J Fish. 2014;7(4):27–36.
24. Safari O. Study on the possibility of optimum diet production of snow trouts (Schizothorax pelzami and Schizothorax zarudnyi) as native species in Iran with aim of sustainable production in the aquaculture systems. FUM. Project no: 33036. Mashhad, Iran; 2016.
25. Ortiz LT, Rebole A, Velasco S, Rodriguez ML, Treviño J, Tejedor JL, et al. Effects of inulin and fructooligosaccharides on growth performance, body chemical composition and intestinal microbiota of farmed rainbow trout (Oncorhynchus mykiss). Aquac Nutr. 2013;19(4):475– 82.
26. Grisdale-Helland B, Helland SJ, Gatlin DM. The effects of dietary supplementation with mannanoligosaccharide, fructooligosaccharide or galactooligosaccharide on the growth and feed utilization of Atlantic salmon (Salmo salar). Aquaculture. 2008;283(1–4):163–7.
27. Bosscher D, Breynaert A, Pieters L, Hermans N. Food based- strategies to modulate the composition of the intestinal. 2009;60(6):5–11.
28. Nakano T. Microorganisms. In: Dietary supplements for the health and quality of cultured fish. Wallingford: CABI; 2007. p. 86–108.
29. Ye JD, Wang K, Li FD, Sun YZ. Single or combined effects of fructo- and mannan oligosaccharide supplements and Bacillus clausii on the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder Paralichth. Aquac Nutr. 2011;17(4).
30. Burr G, Hume M, Neill WH, Gatlin DM. Effects of prebiotics on nutrient digestibility of a soybean-mealbased diet by red drum Sciaenops ocellatus (Linnaeus). Aquac Res. 2008;39(15):1680–6.
31. Gatlin III DM. 12 - Nutrition and Fish Health A2 - Hardy, John E. HalverRonald W. BT - Fish Nutrition (Third Edition). In San Diego: Academic Press; 2003. p. 671–702.
32. Salze G, McLean E, Schwarz MH, Craig SR. Dietary mannan oligosaccharide enhances salinity tolerance and gut development of larval cobia. Aquaculture. 2008;274(1):148– 52.
33. Staykov Y, Spring P, Denev S, Sweetman J. Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss). Aquac Int. 2007;15(2):153–61.
34. Xu ZR, Zou XT, Hu CH. Effects of Dietary Fructooligosaccharide on Digestive Enzyme Activities , Intestinal Microflora and Morphology of Growing Pigs. Asian-Australian J Anim Sci. 2002;15:1784–9.
35. Konstantinov SR, Awati A, Smidt H, Williams BA, Akkermans ADL, De Vos WM. Specific response of a novel and abundant Lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets. Appl Environ Microbiol. 2004;70(7):3821–30.
36. Fric P. Probiotics and prebiotics — renaissance of a therapeutic principle. Cent Eur J Med. 2007;2(3):237– 70.
37. Ringø E, Dimitroglou A, Hoseinifar SH, Davies SJ. Prebiotics in Finfish: An Update. In: Aquaculture Nutrition. John Wiley & Sons, Ltd; 2014. p. 360–400.
38. Cnaani A, Tinman S, Avidar Y, Ron M, Hulata G. Comparative study of biochemical parameters in response to stress in Oreochromis aureus, O. mossambicus and two strains of O. niloticus. Aquac Res. 2004;35(15):1434–40.
39. Ai Q, Xu H, Mai K, Xu W, Wang J, Zhang W. Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea. Aquaculture. 2011 Jul;317(1–4):155–61.
40. Cerezuela R, Cuesta A, Meseguer J, Esteban M. Effects of dietary inulin and heat-inactivated Bacillus subtilis on gilthead seabream (Sparus aurata L.) innate immune parameters. Benef Microbes. 2012 Mar;3(1):77–81.
41. Lin S, Mao S, Guan Y, Luo L, Luo L, Pan Y. Effects of dietary chitosan oligosaccharides and Bacillus coagulans on the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Aquaculture. 2012 Apr;342–343:36–41.
42. Ringø E, Strøm E, Tabachek J-A. Intestinal microflora of salmonids: a review. Aquac Res. 1995;26(Moriarty 1990):773–89.
43. Ringø E, Birkbeck TH. Intestinal microflora of fish larvae and fry. Aquac Res. 1999;30(2):73–93.
44. Perez T, Balcazar JL, Ruiz-Zarzuela I, Halaihel N, Vendrell D, de Blas I, et al. Host-microbiota interactions within the fish intestinal ecosystem. Mucosal Immunol. 2010;3(4):355–60.
45. Ringø E, Gatesoupe F-J. Lactic acid bacteria in fish: A review. Aquaculture. 1998;160(44):177–203.
46. Li P, Burr GS, Gatlin DM, Hume ME, Patnaik S, Castille FL, et al. Dietary supplementation of short-chain fructooligosaccharides influences gastrointestinal microbiota composition and immunity characteristics of Pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system. J Nutr. 2007;137(12):2763–8.
47. Manning TS, Gibson GR. Prebiotics. Best Pract Res Clin Gastroenterol. 2004 Apr;18(2):287–98.
48. Erickson KL, Hubbard NE. Symposium : Probiotic Bacteria : Implications for Human Health Probiotic Immunomodulation in Health and Disease 1 , 2. Am Soc Nutr Sci. 2000;403–9.
49. Perdigon G, Alvarez S, Rachid M, Agüero G, Gobbato N. Immune system stimulation by probiotics. J Dairy Sci. 1995;78(7):1597–606.
50. Bailey JS, Blankenship LC, Cox NA. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poult Sci. 1991 Dec;70(12):2433–8.
51. Smiricky-Tjardes MR, Grieshop CM, Flickinger EA, Bauer LL, Fahey GC. Dietary galactooligosaccharides affect ileal and total-tract nutrient digestibility, ileal and fecal bacterial concentrations, and ileal fermentative characteristics of growing pigs. J Anim Sci. 2003;81(10):2535–45.
52. Van Immerseel F, Russell JB, Flythe MD, Gantois I, Timbermont L, Pasmans F, et al. The use of organic acids to combat Salmonella in poultry: a mechanistic explanation of the efficacy. Avian Pathol. 2006;35(3):182–8.
53. Stoner AW. Assessing Stress and Predicting Mortality in Economically Significant Crustaceans. Rev Fish Sci.
2012 Jul;20(3):111–35.
54. Hai N Van, Buller N, Fotedar R. Effects of probiotics (pseudomonas synxantha and pseudomonas aeruginosa) on the growth, survival and immune parameters of juvenile western king prawns (penaeus latisulcatus kishinouye, 1896). Aquac Res. 2009;40(5):590–602.
55. Tort L. Stress and immune modulation in fish. Dev Comp Immunol. 2011 Dec;35(12):1366–75.
56. Yang B, Wang C, Tu Y, Hu H, Han D, Zhu X, et al. Effects of repeated handling and air exposure on the immune response and the disease resistance of gibel carp (Carassius auratus gibelio) over winter. Fish Shellfish Immunol. 2015;47(2):933– 41.
57. Nikparvar E. Study on the effect of different levels of mannan-oligosaccharide on some biological indices of juvenile pacu (Piaractus brachypomus). Chabahar Maritime University; 2016.
58. Ahmadi I. Study on the effect of different levels of mannan-oligosaccharide on some biological indices of juvenile silver dollar (Metynnis argenteus). FUM. Project no: 23245. Mashhad, Iran; 2015.
59. Soleimani A. Study on the effect of different levels of mannan-oligosaccharide on some biological indices of juvenile zebra fish (Danio rerio). FUM. Project no: 23458. Mashhad, Iran.; 2016.
60. Natt MP, Herrick CA. A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Sci;1952.31:735-738.
61. Brown B. Routine hematology procedures. In: Hematology: Principles and Procedures. Philadelphia, PA: Leo and Fabiger, 1988. pp. 7–122.
62. Blaxhall PC, Daisley KW. Routine haematological methods for use with fish blood. J. Fish Biol.1973; 5:771–781.
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