Preventive Effects of Silymarin on Diclofenac-induced Toxicity in the Domestic Pigeon (Columba livia)

Document Type : Research Article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.

2 Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.

Abstract

This study aimed to evaluate the effects of silymarin on diclofenac-induced acute liver and kidney poisoning in domestic pigeons (Columba livia). The use of NSAIDs leads to adverse drug effects, such as cardiovascular and gastrointestinal hemorrhage and renal side effects. The vast amount of pharmacological attributes possessed by silymarin describes the remarkable content of research aimed at understanding its effect in the remedy of diverse diseases. Fifteen pigeons were randomly assigned into three groups (1, 2, and 3). Group 1 pigeons served as the negative control group and only were given tap water. Groups 2 and 3 were administered diclofenac (15 mg/kg PO q12h) since the start of the study for 24 h. The third group of pigeons was treated with silymarin (35 mg/kg) plus diclofenac, beginning 12 hours after diclofenac exposure, with the silymarin treatment continuing q12h for 48 h. Blood samples were taken from the birds at times 0, 12 h, 24 h, and 48 h of the experiment for serum biochemistry analysis. The results indicated that the treatment of pigeons with silymarin reduced the serum level of AST, ALT, UA, and urea while increasing ALB and TP. Clinical observations also indicated the presence of toxication symptoms, including loss of appetite, diarrhea, and lethargy. These symptoms improved faster in the silymarin group. It can be concluded that silymarin reduces acute liver and kidney damage caused by diclofenac in pigeons.  

Keywords

Main Subjects


1.    Ihedioha JI, Anyogu DC, Ogbonna ME. The effects of silymarin on acetaminophen-induced acute hepatic and renal toxicities in domestic pigeons (Columba livia). Journal of Avian Medicine and Surgery. 2020 Dec;34(4):348-57. Doi:10.1647/1082-6742-34.4.348
2.    Ahsan R, Islam KM, Mussadik A, Haque E. Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in albino rats. Glob J Pharm. 2009;3(3):116–22.
3.    Mallinson TE. A review of ketorolac as a prehospital analgesic. Journal of Paramedic Practice. 2017 Dec 2;9(12):522-6. Doi:10.12968/jpar.2017.9.12.522
4.    Aljuhani N, Elkablawy MA, Elbadawy HM, Alahmadi AM, Aloufi AM, Farsi SH, Alhubayshi BS, Alhejaili SS, Alhejaili JM, Abdel-Halim OB. Protective effects of Ajwa date extract against tissue damage induced by acute diclofenac toxicity. Journal of Taibah University Medical Sciences. 2019 Dec 1;14(6):553-9. Doi:10.1016/j.jtumed.2019.10.002
5.    Lanas A, Chan FK. Peptic ulcer disease. The Lancet. 2017 Aug 5;390(10094):613-24. Doi:10.1016/S0140-6736(16)32404-7.
6.    Moore N, Pollack C, Butkerait P. Adverse drug reactions and drug–drug interactions with over-the-counter NSAIDs. Therapeutics and clinical risk management. 2015 Jul 15:1061-75. Doi:10.2147/TCRM.S79135
7.    Jain A, Yadav A, Bozhkov AI, et al. Therapeutic efficacy of silymarin and naringenin in reducing arsenic-induced hepatic damage in young rats. Ecotoxicol Environ Saf. 2011;74(4):607–14. Doi:10.1016/j.ecoenv.2010.08.002
8.    Razavi BM, Hosseinzadeh H. Saffron as an antidote or a protective agent against natural or chemical toxicities. Daru. 2015;23(1):31. Doi:10.1186/s40199-015-0112-y.
9.    Dorri M, Hashemitabar S, Hosseinzadeh H. Cinnamon (Cinnamomum zeylanicum) as an antidote or a protective agent against natural or chemical toxicities: a review. Drug Chem Toxicol. 2018; 41(3):338–51. Doi:10.1080/01480545.2017.1417995
10.    Federico A, Dallio M, Loguercio C. Silymarin/silybin and chronic liver disease: a marriage of many years. Molecules. 2017;24(2):191. Doi:10.3390/molecules22020191
11.    Surai PF. Silymarin as a natural antioxidant: an overview of the current evidence and perspectives.Antioxidants (Basel). 2015;20(4):204–47. Doi:10.3390/antiox4010204
12.    Sherif IO, Al-Gayyar MM. Antioxidant, anti-inflammatory, and hepatoprotective effects of silymarin on hepatic dysfunction induced by sodium nitrite. Eur Cytokine Netw. 2013;24(3):114–21.
13.    Tyagi A, Agarwal C, Harrison G, et al. Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages. Carcinogenesis. 2004;25(9):1711– 20. Doi:10.1093/carcin/bgh180.
14.    Chung EY, Tat ST. Nonsteroidal anti-inflammatory drug toxicity in children: a clinical review. Pediatric emergency care. 2016 Apr 1;32(4):250-53. Doi: 10.1097/PEC.0000000000000768.
15.    Aronson JK. Non-steroidal anti-inflammatory drugs (NSAIDs). Meyler’s Side Effects of Drugs. 16th ed. Waltham, MA: Elsevier. 2016:236-72.
16.    Rana Abdulla salih. Clinical and Histopathological Study of Diclofenac Sodium Acetylsalicylic Acid Toxic Effect on Liver of Mice. Indian Journal of Forensic Medicine & Toxicology. 2020 Dec. 31 2023 Dec. 23;15(1):2314-21. 
17.    Nethathe B, Chipangura J, Hassan IZ, Duncan N, Adawaren EO, Havenga L, Naidoo V. Diclofenac toxicity in susceptible bird species results from a combination of reduced glomerular filtration and plasma flow with subsequent renal tubular necrosis. PeerJ. 2021 Aug 23;9: e12002. Doi:10.7717/peerj.12002
18.    Ong CK, Lirk P, Tan CH, Seymour RA. An evidence-based update on nonsteroidal anti-inflammatory drugs. Clinical medicine & research. 2007 Mar 1;5(1):19-34. Doi: 10.3121/cmr.2007.698.
19.    Nouri A, Heidarian E, Nikoukar M. Effects of N-acetyl cysteine on oxidative stress and TNF-α gene expression in diclofenac-induced hepatotoxicity in rats. Toxicology mechanisms and methods. 2017 Oct 13;27(8):561-67. Doi:10.1080/15376516.2017.1334732
20.    Eassawy MM, Salem AA, Ismail AF. Biochemical study on the protective effect of curcumin on acetaminophen and gamma-irradiation-induced hepatic toxicity in rats. Environmental Toxicology. 2021 May;36(5):748-63. Doi.org/10.1002/tox.23077
21.    Salimi A, Neshat MR, Naserzadeh P, Pourahmad J. Mitochondrial permeability transition pore sealing agents and antioxidants protect oxidative stress and mitochondrial dysfunction induced by naproxen, diclofenac and celecoxib. Drug research. 2019 Oct;69(11):598-605. Doi: 10.1055/a-0866-9356.
22.    Giridharan R, Lavinya U, Sabina EP. Suppressive effect of Spirulina fusiformis on diclofenac-induced hepato-renal injury and gastrointestinal ulcer in Wistar albino rats: a biochemical and histological approach. Biomedicine & Pharmacotherapy. 2017 Apr 1; 88:8-11. Doi:10.1016/j.biopha.2017.01.032
23.    Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th ed. New York, USA: WH Freeman 2002; 231-273.
24.    Topal I, Özdamar MY, Catakli T, et al. Renoprotective Effect of Taxifolin in Paracetamol-Induced Nephrotoxicity: Emerging Evidence from an Animal Model. J Clin Med 2023; 12(3): 876. Doi:10.3390/jcm12030876 
25.    Ritchie BW, Harrison GJ, Harrison LR. Avian medicine: principles and application. 1998 May 29.
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Volume 16, Issue 3 - Serial Number 36
This issue XML files are being prepared.
September 2024
Pages 67-74
  • Receive Date: 05 March 2024
  • Revise Date: 17 May 2024
  • Accept Date: 10 August 2024