Masoud TalebkhanGaroussi Ali Reza Khosravi Saeideh Pandamoz


Mastitis in cattle is a serious problem which causes considerable economic losses in the dairy cattle herds. The aim of this survey was to identify mycoflorain milk of healthy, clinical and subclinical mastitis of lactating Holstein dairy cows. Milk samples of 154 Holstein dairy cows were collected from 10 dairy cattle herds in suburb of Mashhad-Iran. The treatment groups included 104 lactating dairy cows with clinical (38, 25%) and subclinical (66, 43%) mastitis. Fifty (32%) healthy dairy cows were included as control, as well. Different fungals were isolated from cows with clinical (14%), subclinical (18%) mastitis and healthy animals (15%). There were no significant differences between treatment and control groups (P>0.05). It was shown that milk samples of cows with clinical and subclinical mastitis were infected with 5 different fungal agents. However, milk samples of healthy cows also were contaminated with 5 kinds of fungal agents. Yeast (26%) and Aspergillus (A)fumigatus (18%) were the most common isolated agents. It is concluded that fungal infections (mainly A.) and yeast can occur in mammary glands of lactating Holstein dairy cows with or without mastitis.

Article Details

Aalbaek, B., Stenderup, J., Jensen, H. E.,Valbak, J., Nylin, B., and Huda, A.(1994) Mycotic and algal bovine mastitis in Denmark. Acta Pathologica,Microbiologica et Immunologica
Scandinavica. 102, 451–456.
Awad, F. I., El Molla, A., Fayed, A., Abd el Halim M., and Refai, M. (1980)Studies of mycotic mastitis in Egypt.Journal of the Egyptian Veterinary Medical Association 40,35–41.
Blowey, R., and Edmondson P. (1995)Mastitis control in dairy herds. Farming Press. UK. 41.Brown, J. M., Cowley K. D., Manninen K. I.,and Mcneil M. M. (2007) Phenotypic
and molecular epidemiologic evaluation of a Nocardiafarcinicamastitis epizootic.Veterinary Microbiology 125, 66-72.
Carvirani, S. (2008) Cattle industry and zoonotic risk. Veterinary Research Communication; 32 (suppl), S19-S24.
Costa, E. O., Gandra, C. R., Pires, M. F.,Coutinho, S. D., and Teixeira, C. M.(1993) Survey of bovine mycotic mastitis in dairy herds in the state of Sa˜o Paulo, Brazil. Mycopathologia
124, 13–17.
Fang, W., Shi, M., Huang, L., Shao, Q., and Chen, J. (1993) Growth of lactobacilli,Staphylococcus aureus and Escherichia
coli in normal and mastitic milk and whey. Veterinary Microbiology 37,115–125.
Fernandez, A., Belio, R., Ramos, J.J., Sanz, M.C., and Saez, T. (1997) Aflatoxins and their metabolites in the tissues, feces
and urine from lambs feeding on an aflatoxin-contaminated diet. Journal of the Science of Food and Agriculture74,161-168.
Hall, R. F., Harrison, L. R., and Colvin, B. M.(1989) Aflatoxicosis in cattle pastured in a field of sweet corn. Journal of the
American Veterinary Medical Association194, 938.
Jensen, H. E., Basse, A., and Aalbaek, B. (1989)Mycosis in the stomach compartments of cattle. Acta Veterinaria Scandinavica 30,409–423.
Jensen, H. E., Olsen, S. N., and Aalbaek, B.(1994) Gastrointestinal aspergillosis and zygomycosis of cattle. Veterinary Pathology 31, 28-36.
Krukowski, H., Tietze, M., Majewski, T., and Rozanski, P. (2000) Survey of yeast mastitis in dairy herds of small-type farms in the Lublin region, Poland.Mycopathologia 150, 5–7.
Mclean, M., and Dutton M. F. (1995) Cellular interactions and metabolism of aflatoxin: an update. Pharmacology& Therapeutics 65, 163-92.
Nicholls, T. J., Barton, M. G., and Anderson,B. P. (1981) An outbreak of mastitis in a dairy herd due to Pseudomonas
aeruginosa contamination of dry-cow therapy at manufacture. The Veterinary Record 108, 93-96.
Philpot W. N. (1984) Economics of mastitis control. The Veterinary Clincs of North America Large Animal Practice 6, 233-45.
Philpot, W. N., and Nickerson, S. C. (1991)Mastitis: counter attack. Babson Bros Publication. USA. 22.
Radostitis, O., Gay, C. C., Hinchcliff, K. W.,and Constable P. D. (2007) Veterinary medicine. 10th ed. Saunders Elsevier.
USA. 675, 685, 727.
Richard, J. L., McDonald, D. V. M., Fichtner,R. E., and Anderson, A. J. (1980)Identification of yeasts from infected bovine mammary glands and their experimental infectivity in cattle.
American Journal of Veterinary Research 12, 1991–1994.
Santos, R. C., and Marin, J. M. (2005)Isolation of Candida spp. from mastitic bovine milk in Brazil. Mycopathologia 159, 251-253.
Thrusfield, M. (2005) Veterinary Epidemiology. 3rd ed. Blackwell
Science Publication. 233.
Vestweber, J. G., and Leipold, H. W. (1995)Pulmonary and mammary aspergillosis in a dairy cow. Canadian Veterinary
Journal 35, 780.
How to Cite
TalebkhanGaroussiM., KhosraviA. R., & PandamozS. (2013). Milk MycofloraSurvey of Dairy Cows with or without Mastitis. Iranian Journal of Veterinary Science and Technology, 4(1), 69-74. https://doi.org/10.22067/veterinary.v4i1.26469
Original Articles