Amin Tavassoli Nasser Mahdavi-Shahri Maryam M. Matin Masoud Fereidoni Fahimeh Shahabipour


Extracelllar matrix (ECM) materials from mammalian tissues have been broadly used for tissue engineering for animal models. While decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. In This study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construct for cartilage substitution. For this purpose, different decellularization protocols including freeze/thaw cycles followed by treatment with various concentrations of sodium dodecyl sulfate (SDS) were used. Decellularization was analyzed by histological examination including hematoxylin and eosin and picrosirius staining. Treatment with 2%SDS for 5 to 8h followed by 4% SDS for3h hada significant effect on decellularization process with the minimal effect on the collagen contents. On the other hand, Treatment with 1% SDS had no effect on cell removal and use of 8% SDS for 5 to 8 h resulted in complete elimination of cells and significant decrease in cartilage matrix and collagen contents. This study provides a technique to produce acellular ECM derived from articular bovine cartilage whichmay serve as a xenogenicscaffold for cartilage tissue engineering.

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Brittberg, M., Linda hl. A,, Nilsson, A.,Ohlsson, C., Isaksson, O., Peterson, L.(1994) Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. The New
England Journal of Medicine 331, 889-895.
Buckwalter, J.A. (1983) articular cartilage.Instructional Course Lectures 32, 349–370.
Chen, R.N., Ho, H.O., Tsai, Y.T., Sheu, M.T.(2004) Process development of an acellular dermal matrix (ADM) for biomedical applications. Biomaterials 25, 2679-2686.
Elder, B.D., Kim, D.H., Athanasiou, K.A.(2010) Developing an articular cartilage decellularization process toward facet joint cartilage replacement. Neurosurgery 66, 722-727.
Furukawa, T., Eyre, D.R., Koide, S.,Glimcher, M.J. (1980) Biochemical studies on repair cartilage resurfacing
experimental defects in the rabbit knee.The Journal of Bone and Joint Surgery 62, 79-89.
Ghazavi, M.T., Pritzker, K.P., Davis, A.M.,Gross, A.E. (1997) Fresh osteochondral allografts for post-traumatic osteochondral defects of the knee.The Journal of Bone and Joint Surgery
(British Volume) 79, 1008–1013.
Gilbert, T.W., Sellaro, T.L., Badylak, S.F.(2006) Decellularization of tissues and organs. Biomaterials 27, 3675-3683.
Gilbert, T.W., Stolz, D.B., Biancaniello, F.,Simmons-Byrd, A., Badylak, S.F.(2005) Production and characterization of ECM powder: implications for tissue engineering applications. Biomaterials 26, 1431-1435.
Huber, M., Trattnig, S., Lintner, F. (2000)Anatomy, biochemistry, and physiology of articular cartilage.Investigative Radiology 35, 573–580.
Junqueira, L.C., Bignolas, G., Brentani, R.R.(1979) Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections.Histochemical Journal 11,
Kelley, T.F., Sutton, F.M., Wallace, V.P.,Wong, B.J. (2002) Chondrocyte repopulation of allograft cartilage: a preliminary investigation and strategy for developing cartilage matrices for
reconstruction. Otolaryngology - Head and Neck Surgery 127, 265-270.
Kish, G., Modis, L., Hangody, L. (1999) Osteochondral mosaic plasty for the treatment of focal chondral and osteochondral lesions of the knee and talus in the athlete. Rationale,
indications, techniques, and results.Clinical of Sports Medicine 18,
Kropp, B.P., Eppley, B.L., Prevel, C.D., Rippy, M.K., Harruff, R.C., Badylak,S.F., Adams, M.C., Rink, R.C.,Keating, M.A. (1995) Experimental assessment of small intestinal submucosa as a bladder wall substitute.Urology 46, 396-400.
Macchiarini, P., Jungebluth, P., Go, T.,Asnaghi, M.A., Rees, L.E., Cogan,T.A., Dodson, A., Martorell, J., Bellini,S., Parnigotto, P.P., et al. (2008)Bovine articular cartilage decellularized matrix as a scaffold Clinical transplantation of a tissueengineered
airway. Lancet 372, 2023-2030.
Schenke-Layland, K., Vasilevski, O., Opitz,F., Konig, K., Riemann, I., Halbhuber,K.J., Wahlers, T., Stock, U.A. (2003) Impact of decellularization of xenogeneic tissue on extracellular
matrix integrity for tissue engineering of heart valves. Journal of Structure Biology 143, 201-208.
Schmidt, C.E., Baier, J.M. (2000) Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering.
Biomaterials 21, 2215-2231.
Steadman, J.R., Rodkey, W.G., Briggs, K.K.,Rodrigo, J.J. (1999) The micro fracture technicin the management of complete
cartilage defects in the knee joint.Orthopade 28, 26–32.
Woods, T., Gratzer, P.F. (2005) Effectiveness of three extraction techniques in the development of a decellularized boneanterior
cruciate ligament-bone graft.Biomaterials 26, 7339-7349.
Yang, Q., Peng, J., Guo, Q., Huang, J., Zhang, L.,Yao, J., Yang, F., Wang, S., Xu, W.,Wang, A., Lu, S. (2008) A cartilage ECMderived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells. Biomaterials 29,
How to Cite
TavassoliA., Mahdavi-ShahriN., MatinM. M., FereidoniM., & ShahabipourF. (2014). Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering. Iranian Journal of Veterinary Science and Technology, 4(1), 1-8. https://doi.org/10.22067/veterinary.v4i1.17878
Original Articles