##plugins.themes.bootstrap3.article.main##

Fatemeh Bibak Gholam Ali kalidari Jamshid Razmyar Mehrnaz Rad

Abstract

Mycoplasmosis is one of the most important diseases in the poultry industry. Its causative agent, mycoplasma has various species, which two of them, Mycoplasma gallisepticum (MG)
and Mycoplasma synoviae (MS) are the most important species. Due to the enormous losses in the production farms of industrial poultry, achieving a rapid, accurate and definite
diagnosis of mycoplasma is of great importance. An early and definite diagnosis can guarantee the farm management on keeping herd health. In many countries such as Iran, the
disease and its complications have still remained as a serious problem. Given this issue, we decided to identify the mycoplasma infection from broiler poultry flocks through culture
method. 150 carcasses of broiler chicken belonging to 50 broiler flocks were sampled in which the signs of air sacs involvement and secretions in the airways, trachea and bronchi
were seen. Samples taken from trachea, palatine cleft, nasal passages and air sacs, were cultivated into PPLO liquid medium using membrane filters (0.45 micron). They were
incubated at 37 °C and were examined for pH (color) changes for every 48 hours. During the first 24 hours after cultivation, every color change to yellow or dark visible with the bare eye
was considered as bacterial contamination, therefore, the contaminated samples were removed from the incubator. The color change in the liquid media was compared with the uninoculated medium as negative control. If a color change was observed in the liquid media after 48h, subculture was done in the PPLO agar. The plates were incubated at 37 °C for 14 days. They were examined for mycoplasma colonies using a microscope with magnification of 10 in every other day. The results showed that out of 150 samples obtained from 50 broiler flocks,
16 (10.66%) were positive for mycoplasma, while in terms of contamination, 4 flocks (8%) were positive. The contamination of positive cultures was finally confirmed through PCR method with universal primer.

Article Details

References
Bradbury, J m. (1998) Recovery of Mycoplasmas from birds. In: Miles Rj & Nicholas Raj, eds. Mycoplasma protocols, Humana press, Totown.USA. 45-52.
Fritz, B.A., Thomas, C.B., Van Ess, P and Yuill, T.M.(1991)Comparison of a modified Edward-type medium and a modified SP4-type medium for primary isolation of Mycoplasma gallisepticum from chickens vaccinated with the F strain of MG. Avian Diseases 35, 591-598.
Ghaleh Golab Behbahan, N., Asasi, K.,Afsharifar, A.R and Pourbakhsh, S.A.(2005) Isolation and detection of Mycoplasma gallisepticum by polymerase chain reaction and restriction fragment length polymorphism.Iranian Journal of veterinary research 6(2),12.
Hanif A, Najeeb M.I.(2007) Comparison of conventional bacterial isolation, Rapid slide agglutination and Polymerase Chain Reaction for detection of Mycoplasmagallisepticum in breeder flocks.Pakistan Journal of life and social sciences 5(1-2),1
Hosseini H, Bozorgmehrifard M.H.2007; Molecular studies on Mycoplasma gallisepticum isolated from poultry.Journal of Infectious Diseases.186.
Kleven, S.H. (1998) Mycoplasmosis. In: Swayne, D.E., Glisson, J.R., Jackwood, M.W., Pearson,J.E., Reed, W.M.(Eds.), A laboratory manual for the isolation and identification of avian pathogens. 4th Ed, American Association of avian pathologists, pp.74-80.
Khiari, A., Landouls, A., Aissa, I.H., Mlik, B.,Amouna, F., Ejlassi, A and Mardassi,B.A.(2011) Isolation of Mycoplasma meleagridis from Chickens. Avian Diseases 55,8–12.
Pourbakhsh, S.A., Shokri, G.R., Banani, M.,Elhamnia, F and Ashtari, A.(2010) Detection of Mycoplasma synoviae infection in broiler breeder farms of Tehran province using PCR and culture methods. Archives of Razi Institute 65(2), 75-81.
Stanley H. Celeven (2008) Mycoplasmosis, In Saif Y.M, (Ed.). Diseases of poultry. Blackwell publishing. PP. 805-868.
Razin, S., Yogev, D and Naot, Y. (1998)Molecular biology and pathogenicity of Mycoplasmas. Microbiology and Molecular Biology Reviews 62,1094-1156.
Wong-Lee, JG and Lovett, M. (1993) Rapid and sensitivePCR method for identification of ycoplasmaspecies in tissue culture. In: Persing D, ed.DiagnosticMolecular and Microbiology Principles and Applications. Washington DC: American Societyfor
Microbiology,257-260.
Yilmaz, F., Timurkaan, N., Kilic, A.,Kalender, H and Kilinc U.(2011)Detection of Mycoplasma synoviae and Mycoplasma gallisepticum in chickens by immunohistochemical, PCR and culture methods. Microbiology and Molecular Biology Reviews 162(2), 79-86.
How to Cite
BibakF., kalidariG. A., RazmyarJ., & RadM. (2013). Isolation of Mycoplasma spp. from broiler flocks with respiratory syndrome in Mashhad, Iran. Iranian Journal of Veterinary Science and Technology, 5(1), 11-18. https://doi.org/10.22067/veterinary.v5i1.16248
Section
Original Articles