Macroscopic evaluation of wound healing activity of the Persian shallot, Allium hirtifolium in rat

Document Type : Research Articles

Authors

Ferdowsi University of Mashhad

Abstract

Wound is a break in the outer layer of skin. There are several different methods to provoke wound healing process, consisting topically or orally administration of medicinal drugs or
herbal remedies. Persian shallot, Allium hirtifolium which belongs to the same genus of garlic,naturally grows in different parts of Iran. In this study, we have avaluated the wound healing
activity of hydro-alcoholic extract of A .hirtifolium Boiss. Four bilateral full-thickness wounds (2 on each side) were made on the dorsal area of four adult albino rats weighing 165±35 gr under general anesthesia. Right side wounds were treated in experimental groups,while left side wounds were considered as control littermates. One day after surgery, a gellike 1:1 mixture of A. hirtifolium extract and methylcellulose were topically applied (100 mg/kg/day) to the experimental wounds while the wounds in control groups were treated with the extract-free gel for 12 days. At days 1, 3, 6, 9 and 12, digital photos of wounds were taken using of Scion image software, for analyzing the percentage of wound contraction, epithelialisation and speed of healing. The results revealed that A. hirtifolium can accelerate
wound healing by increasing the rate of epithelialisation. We concluded that A. hirtifolium extract may be clinically useful in management of open wounds treatment procedures.

Keywords

Main Subjects

References

Ashrafi, F., Akhavan Sepahi, A. and Kazemzadeh, A. (2004) Effect of aqueous extract of shallot (Allium ascalonicum) on inhibition of growth of Pseudomonas aeroginosa. Iranian J.
Pharm. Res. 3, 71-71.
Barile, E., Bonanomi, G., Antignani, V.,Zolfaghari, B., Sajjadi, S. E., Scala, F.and Lanzotti, V. (2007) Saponins from
Allium minutiflorum with antifungul activity. Phytochemistry 68, 596-603.
Fritsch, R. M., Salmaki, y., zarre, S. and Jaharchi, M. (2006) The genus Allium Alliaceae) in Iran: Current state, new taxa and new records. Rostaniha 7 (suppl. 2), 254-281.
Ghahreman, A. (1984) Color Atlas of Iranian Plants, Institute of Forestries and Grasslands, Botany Division 5, 512-512.
Ghodrati Azadi, H., Ghaffari, S.M., Riazi,G.H., Ahmadian, S. and Vahedi, F.(2008) Antiproliferative activity of chloroformic extract of Persian Shallot,Allium hirtifolium, on tumor cell lines.
Cytotechnology 56(3), 179-185.
Ghodrati Azadi, H., Riazi, G.H., Ghaffari, S.M. and Ahmadian, S. (2009) Effects of Allium hirtifolium (Iranian shallot) and
its allicin on microtubule and cancer cell line. African Journal of
Biotechnology8 (19), 5030-5037.
Gupta, P.K. and Rustgi, S. (2004) Molecular markers from the transcribed expressed regions of genome in higher plants.
Funct. Integr.Genome. 4, 139–162.
Hostettmann, K., Potterat, O. and Wolfender, J.L. (1998) The Potential of Higher Plants as a Source of New Drugs.
Chimia International Journal for Chemistry 52, 10-17.
Jafarian, A., Ghannadi, A. and Elyasi, A.(2003) The Effects of Allium hirtifolium Boiss. on Cell-Mediated Immune Response in Mice. Iranian Journal of Pharmaceutical Research 2,
51-55.
Krishnan , P. (2006) The scientific study of herbal wound healing therapies:Current state of play Current Anesthesia & Critical Care 17 (1-2),21-27.
Kuttan, G. (2000) Immunomodulatory effect of some naturally millipore sulphur containing compounds. J.Ethnopharmacol. 72, 93- 99.
Martin, P. (1996) Wound healing-aiming for perfect skin regeneration. Science 276,75-81.
Mozaffarian, V. (1996) A Dictionary of Iranian Plant Names. Farhange Moaser Publication, Tehran. Panus, P. (2008) Pharmacology for the Physical Therapist, first edn., McGraw-
Hill Incorporated, New York.
Phillipson, J. D. (2001) Phytochemistry and medicinal plants Phytochemistry 56(3), 237-243.
Polasek, J., Queiroz, E.F. and Hostettmann, K.(2007) On-line identification of phenolic compounds of Trifolium species using HPLC-UV-MS and postcolumn UV-derivatisation.Phytochemical Analysis 18 (1), 13–23.
Rechinger, K.H. (1984) Flora Iranica,Alliaceae. Akademische Druck, Univ.Verlagsanstalt Graz, Austria 76, 85.
Reddy, J.S. Rao, P.R. and Reddy, M.S.(2002) Wound healing effects of Heliotropium indicum, Plumbago zeylanicum and Acalypha indica in rats Journal of Ethnopharmacology 79 (2),
249-25.
Rubatzky, V.E. and Yamaguchi, M. (1997) World Vegetables, Principles,Production and Nutritive Values.Second edn., Chapman and Hall/International Thompson Publishing, New York.
Salunkhe, D.K. and Kadam, S.S. (1998)Handbook of Vegetable Science and Technology. Marcel Dekker, Inc., New
York. pp 721-721.
Sardari, K., Dehghan, M.M., Mohri, M.,Emami, M.R., Mirshahi, A., Maleki,M., Najar Barjasteh, M. And Aslani,M. (2006) Macroscopic aspects of wound healing (contraction and epithelialisation) after topical administration of allicin in dogs.Comp. Clin. Pathol. 15, 231-235.
Shukla, A. and Mossman, B.T. (2008) Cell Signaling by Oxidants: Pathways Leading to Activation of Mitogenactivated
Protein Kinases (MAPK) and Activator Protein-1 (AP-1). Current
Topics in Membranes 61, 191-20.
Tanya, J.S. and Martin, P. (2009) Wound repair at a glance. Journal of Cell Science. 122, 3209-3213.
Taran, M., Rezaeian, M. and Izaddoost, M.(2006) In vitro Antitrichomonas Activity of Allium Hirtifloium (Persian
Shallot) in Comparison with Metronidazole. Iranian Journal of
Public Health 35 (1), 92-94.
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  • Receive Date: 12 March 2012
  • Accept Date: 12 March 2012

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