Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X3120110801Comparative pathomorphological study of rickets types in broiler chickens1102565710.22067/veterinary.v3i1.11875ENIvan DinevTrakia University0000-0002-0197-3491Journal Article20120312The purpose of this study was to determine the incidence of rickets types in broiler chickens on the basis of gross and microscopic lesions in field clinical cases with regard to differential pathomorphological diagnosis.Clinical, serological, gross anatomy and histological investigations were carried out in broiler chickens with signs of rickets. The survey included 12 finisher broiler flocks (ten Ross 308 and two Cobb 500 flocks) from 4 different farms.Gross lesions in chickens from two of farms were indicative of hypophosphataemic rickets and the other two- of hypocalcaemic rickets types. Microscopic lesions correlated to<br />macroscopic ones. The results of investigations showed that lesions resulting from calcium deficiency were very different from those occurring after phosphorus deficiency or calcium excess. It was suggested that macroscopic lesions in the proximal tibiotarsus could be used to distinguish field cases of Ca-deficiency and P-deficiency rickets in broiler chickens. The lesions in other leg parts (femur and tarsometatarsus) were not consistent with regard to differential diagnosis of rickets types due to the insufficient lesions in these bones.Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X3120110801Clinical and pathological study of experimentally- induced yew (Taxus baccata) poisoning in sheep11222567610.22067/veterinary.v3i1.11876ENMohammad Reza AslaniFerdowsi University of Mashhad0000-0001-5479-676XAlireza Taghavi-RazavizadehFerdowsi University of MashhadAhmad Reza MovassaghiFerdowsi University of MashhadMehrdad MohriFerdowsi University of MashhadAbolghassem NabipourFerdowsi University of Mashhad0000-0002-2129-8382Ali RezakhaniShiraz UniversitySamaneh Yaghoobi-FazFerdowsi University of MashhadAli Andalib-MoghadamFerdowsi University of MashhadFatemeh AhmadiFerdowsi University of MashhadJournal Article20120312Acute yew (Taxus baccata) intoxication was induced in 7 adult sheep by oral administration of the plant materials at the dose of 2.5g/kg body weight. Blood samples for hematology and serum biochemistry were collected before and after the induced intoxication.Electrocardiography was performed using a base-apex lead. Intoxicated animals displayed lethargy, depression, bradycardia and varying intensity of heart sounds. The ECGs of intoxicated sheep showed a variety of arrhythmias and abnormalities including multifocal ventricular tachycardia, idioventricular and idiojunctinal rhythm and QRS and T widening.<br />Four sheep died within 4-16 hours after yew administration. Significant increase of white blood cells, total neutrophil counts and serum sodium concentration were observed. Post mortem examination and histological evaluation were done with special regards on tissue changes of the myocardium and conduction system. There were hyperemia, focal hemorrhage and multifocal nonsuppurative interstitial myocarditis with mild focal cardiac muscle cell degeneration and necrosis in the affected hearts which appeared more prominent in the right atrium. Histopathological examination of cardiac conduction system also revealed moderate to severe interstitial edema with inflammation of the SA-node, AV-node, the bundle branches and His-bundle in all cases which was most severe in the AV-node and the His-bundle. Oral administration of dried leaves of T. baccata, at the dose of 2.5g/kg can induce acute toxicity, manifested clinically by a variety of cardiac arrhythmia and myocardial lesions<br />including special conduction system in pathology.Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X3120110801Evaluation of Babesia Spp. Infection among Native Horses in Quezon Province, Philippines, Using Molecular and Microscopical Examination23302570310.22067/veterinary.v3i1.11877ENFletcher P.Del ValleUniversity of the Philippines Los Baños0000-0003-2839-0568Abigail M.BaticadosUniversity of the Philippines Los BañosVaren N.BaticadosUniversity of the Philippines Los BañosJournal Article20120312The possible existence of equine piroplasmosis was investigated in the province of Quezon, Philippines. Blood samples of 269 horses from selected municipalities of Quezon Province were gathered. Parasitological test namely blood parasite examination was employed and the test yielded negative results for the presence of the parasite. Similarly, polymerase chain reaction assay was performed using Babesia genus-specific primers. PCR assay also resulted to all samples being negative for the disease.<br />The negative results are suggestive of a variety of causes specifically; low parasite concentration during sample collection, localization of the parasite in the endothelium, latent stage of infection, acquired immunity, drug intervention and true negative or complete absence of parasite in the blood. The abovementioned reasons are either characterized by very low parasitemia, no parasitemia or eventually complete disappearance of the parasites in the blood, all of which subsequently confounds the observance of the parasite.Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X3120110801Macroscopic evaluation of wound healing activity of the Persian shallot, Allium hirtifolium in rat31382572810.22067/veterinary.v3i1.11878ENHamideh Ghodrati AzadiFerdowsi University of MashhadBehrooz FathiFerdowsi University of Mashhad0000-0003-2795-6443Hossein Kazemi MehrjerdiFerdowsi University of Mashhad0000-0002-5593-6864Mohsen MalekiFerdowsi University of Mashhad0000-0001-7064-8193Hanieh ShaterzadehFerdowsi University of MashhadMaryam AbyaziFerdowsi University of MashhadJournal Article20120312Wound is a break in the outer layer of skin. There are several different methods to provoke wound healing process, consisting topically or orally administration of medicinal drugs or<br />herbal remedies. Persian shallot, Allium hirtifolium which belongs to the same genus of garlic,naturally grows in different parts of Iran. In this study, we have avaluated the wound healing<br />activity of hydro-alcoholic extract of A .hirtifolium Boiss. Four bilateral full-thickness wounds (2 on each side) were made on the dorsal area of four adult albino rats weighing 165±35 gr under general anesthesia. Right side wounds were treated in experimental groups,while left side wounds were considered as control littermates. One day after surgery, a gellike 1:1 mixture of A. hirtifolium extract and methylcellulose were topically applied (100 mg/kg/day) to the experimental wounds while the wounds in control groups were treated with the extract-free gel for 12 days. At days 1, 3, 6, 9 and 12, digital photos of wounds were taken using of Scion image software, for analyzing the percentage of wound contraction, epithelialisation and speed of healing. The results revealed that A. hirtifolium can accelerate<br />wound healing by increasing the rate of epithelialisation. We concluded that A. hirtifolium extract may be clinically useful in management of open wounds treatment procedures.Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X3120110801Partial sequence determination of a cDNA encoding microneme 5 protein of Eimeria necatrix isolated in Khuzestan province, Iran39482575510.22067/veterinary.v3i1.11879ENShahrooz MasaeliShahid Chamran University of AhvazMansour MayahiShahid Chamran University of Ahvaz0000-0002-4084-0990Abbas JolodarShahid Chamran University of Ahvaz0000-0001-9994-2281Hossein HamidinejatShahid Chamran University of AhvazMasoud Reza Seifi Abad ShapouriShahid Chamran University of AhvazJournal Article20120312Micronemes are secretory organelles of the invasive stages of apicomplexan parasites and contain proteins that are important for parasite motility and host cell invasion. Even though <br />coccidiosis is a complex disease that can be caused by any combination of mainly seven species, most of the molecular researches concerning characterization of host and parasite<br />genes and proteins have been limited to Eimeria tenella. The present study describes isolation and purification of Eimeria necatrix oocysts that can be used for an inexpensive and simple<br />total RNA extraction method to partial sequence determination of a cDNA encoding microneme 5 protein. Using the extracted total RNA as template and oligo(dT) as primer, cDNA was synthesized. In order to amplify cDNA encoding the micronem 5 protein (EnMIC- 5), RT-PCR was applied with the specific primers based on the known EST sequence.Following amplification, the unique and thick 758 bp fragment was seen. Domain analysis of<br />EnMIC-5 revealed that the sequence contains the conserved domain of PAN/APPLE superfamily between amino acid resides 130 to 201. This domain has strong similarity to the<br />adhesive plasma pre-kallikrein. Despite sequence similarity of EnMIC-5 with those sequences in database, differences may represent some allelic polymorphism.Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X3120110801Dirofilaria immitis infection in a Dachshund dog: Diagnosis and Treatment49562577910.22067/veterinary.v3i1.11880ENMohammad Hossein Razi JalaliShahid Chamran University of AhvazBahman MosallanejadShahid Chamran University of Ahvaz0000-0001-9150-7920Reza AvizehShahid Chamran University of AhvazJournal Article20120312Dirofilaria immitis is a filariid worm which typically lives as adult in the right ventricle of the heart and the pulmonary arteries of the dog, causing the canine heartworm disease.Therapy of canine dirofilariosis due to Dirofilaria immitis is indicated for dogs suffering<br />from clinical signs of the disease (such as chronic cough). The present paper describes diagnostic and treatment features of a D. immitis affected dog in Ahvaz district, Southwest of<br />Iran. The dog had coughing, tachypnea, labored breathing, panting, hind limb edema,weakness, and exercise intolerance, at the time of referral. Auscultation revealed grade III/VI<br />systolic murmur over the left apex of the heart. Giemsa stained blood smears containing microfilaria was morphologically identified as D. immitis. CBC revealed an inflammatory leukogram and mild anemia. Combined therapy with ivermectin (440 μg/kg as single dose),levamisol (10 mg/kg q 24 h for 10 days) and aspirin (10 mg/kg q 12 h for 10 days), during 2 stages, was effective. The number of microfilaria dropped from 1250±50/ml blood pretreatment to 150±10/ml (following the first treatment stage, after 10 days) and reached 0 a day after the second treatment. The animal remained negative regarding D. immitis microfilaremia<br />during a follow-up period of 180 days. This record confirms the efficacy of ivermectin with levamisol and aspirin in the treatment of adult heartworm infection in a dog.Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X3120110801Lipid rich carcinoma and solid carcinoma in mammary gland of a dog: histopathologic and immunohistochemical features57642579810.22067/veterinary.v3i1.11881ENAnahita RezaieShahid Chamran University of AhvazSaleh EsmaeilzadehShahid Chamran University of AhvazHadi NaddafShahid Chamran University of AhvazJournal Article20120312Lipid rich carcinoma is rare in dog and women and is characterized by cells that have an abundant foamy cytoplasm. A 10 year old, mix breed female dog suffered two subcutaneous masses in ventral abdomen was referred to surgery department of veterinary hospital of Ahvaz Shahid Chamran University. Surgical excision and microscopic examination of masses were done. After grading by Elston and Ellis method, istochemical staining was performed such as PAS, Cong red and Oil Red O.Immunohistochemical detection of pKi67, P53, cerbB2<br />and factor VIII – related antigen were carried out.icroscopically, the masses were two parts; first compose of big cells with vacuolated cytoplasm and euchromatin nuclei and<br />second part cells with scant cytoplasm and pleomorphic nuclei and obvious membrane. Grade of tumor was Grade III, poorly differentiated. Positive reaction was seen in vacuolated<br />cytoplasm for Oil Red O. Neoplastic cells of lipid rich area demonstrated intense immunoreactivity for pKi67. All tumor cells lacked p53 and c-erbB2. This is the first report of coincidence of lipid rich carcinoma and solid carcinoma in a dog with description of<br />histopathologic and immunohistochemical characteristics.