Ferdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701Prevalence of canine cutaneous neoplasms from Shiraz, Iran163021110.22067/veterinary.v1i11.79564ENMohammad Abbaszadeh HasiriShiraz UniversityFatemeh NamaziShiraz University0009-0006-8148-3535Forough Zarei KordshouliShiraz UniversityJournal Article20190306The present study was conducted on 42 dogs with a histopathological diagnosis of skin neoplasia presented in the Shiraz University Veterinary Clinic from April 2012 to December 2017. All cases were reviewed, excluding the mammary gland neoplasms. The histopathological type, prevalence, sex, age, breed and site distribution of the neoplasms were described. In addition, previous studies on canine skin tumors from other geographic regions were evaluated and compared with the results of the present study. Fifteen different histopathological types of tumor were diagnosed. The prevalence of epithelial, mesenchymal, melanocytic and lymphohistiocytic tumors was 61.9%, 35.7%, 2.4% and 0%, respectively. Th e three most common tumors were sebaceous gland adenoma (21.42%), squamous cell carcinoma (11.9%), and lipoma (11.9%). The incidences of these tumors were more than other researches. Although there is no obvious explanation for these geographical differences, the possible reasons may be the geographical locations, environmental infl uences, and the study population and breed. Terriers were the most common type of the dogs in this study (34.4%). The present findings about the dogs age and various skin tumors and the anatomical locations indicates that there is no signifi cant variation in these important parameters among the Iranian dogs and dogs from other parts of the world.https://ijvst.um.ac.ir/article_30211_278d3ecd1fc85f6b2b68f826f8e3e704.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701Experimental infection of pheasants with a velogenic chicken isolate of Newcastle disease virus7123024410.22067/veterinary.v1i11.80546ENRamezan Ali JafariShahid Chamran University of Ahvaz0000-0003-1998-8699Anahita RezaieShahid Chamran University ofZahra BoroomandShahid Chamran UniversityMansoor MayahiShahid Chamran UniversityReza ZareShahid Chamran UniversityJournal Article20190506Newcastle disease (ND) is a highly contagious infection of many avian species, causing enormous losses in poultry production worldwide. The objective of this study was to reveal the clinical feature, virus shedding, and immune response following infection with a velogenic chicken isolate of Newcastle disease virus (NDV) in susceptible and vaccinated pheasants. Eighty day-old pheasant chicks were allotted to four groups. At 30 days of age, the birds in groups 1 and 3 were vaccinated with B1 strain via eye drop. Two weeks later, each bird in groups 1 and 2 was inoculated with 100 μL(50 μL/eye) of NDV-infected allantoic fluid containing 105 EID50 of viral inoculum. All groups were inspected daily for three weeks. Swab samples were taken at different time points and verifi ed for NDV infection by using reverse-transcription polymerase chain reaction (RT-PCR). Serological examination was also made by haemagglutination-inhibition assay. Clinically, watery mucoid feces was observed only in one case among the vaccinated challenged birds, whereas the unvaccinated challenged birds showed anorexia, mild depression and head deviation. Out of 20 birds in group 2, one case (5%) died. Based on RT-PCR, virus shedding was only observed among the unvaccinated birds from 5 to 14 days aft er challenge. The NDV was detected more in tracheal swabs (40%) than in cloacal swabs (30%). The infected birds showed a high seroconversion. In conclusion, the velogenic NDV circulating in Iranian chicken flocks has a low pathogenicity for pheasants, and ocular vaccination with B1 strain could provide a good protection.https://ijvst.um.ac.ir/article_30244_fbd4298595164b68fafcec9d040eb2fa.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701The Eff ect of resistance training and growth hormone injection on circulating IGF-1 and IGFBP-3 levels in a rat model13183026410.22067/veterinary.v1i11.78504ENBehnam RoozbehFerdowsi University of MashhadMahtab MoazamiFerdowsi University of MashhadAmir RashidlamirFerdowsi University of MashhadZahra MoosaviFerdowsi University of Mashhad0000-0002-8388-2699Ali JavadmaneshFerdowsi University of Mashhad0000-0001-6016-5905Journal Article20190112Growth hormone has mitotic and anti-apoptotic effects which may increase proliferation and transformation of cells when it is expressed aberrantly. This study investigated the effects of resistance training and growth hormone injection on circulating IGF-1, IGFBP-3 levels and IGF-1/IGFBP-3 ratio in male Wistar rats. Thirty-two male Wistar rats were randomly assigned to a control group (C, n = 8), a resistance training group (RT, n = 8), a growth hormone injection group (GI, n = 8) and a resistance training + growth hormone injection group (RG, n = 8). The resistance training protocol comprised of climbing a ladder (5 days/week, 3 sets/5 reps) while carrying a weight suspended from the tail. The growth hormone (2 mg/kg/day, 5 days/week) was injected before an exercise session. Serum IGF-1, IGFBP-3 levels, and IGF-1/IGFBP-3 ratio were measured after 8 weeks. One-way ANOVA analysis was used for comparison of serum IGF-1 and IGFBP-3 levels between groups. Serum IGF-1 levels and IGF-1/IGFBP-3 ratio signifi cantly decreased, but serum IGFBP-3 levels showed no significant change in the RT group compared to the C group. Also, both serum IGF-1 and IGFBP-3 levels and IGF-1/IGFBP-3 ratio in GI and RG groups significantly increased compared to the other groups. In conclusion, resistance training decreases serum IGF-1 levels and/or IGF-1/IGFBP-3 ratio in normal condition. On the other hand, the growth hormone injection with and without the resistance training increases serum IGF-1 levels and IGF-1/IGFBP-3 ratio which could be noted as a condition with a higher risk of neoplasm.https://ijvst.um.ac.ir/article_30264_82ba9e85d0b0abe787c9adfaabf8a0c1.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701Interactive effects of peripheral and central administration of LPS with inhibition of CRF receptors on food intake in neonatal chicks19253030710.22067/veterinary.v1i11.76494ENRazieh Kooshkishahid bahonar university0000-0001-6024-4507Mehdi Abbasnejadshahid bahonar university0000-0003-2570-5288Hossein Jonaidishahid bahonar university0000-0002-5420-491XMohadese Soltaninejadshahid bahonar university0009-0004-6373-1297Abdolhamid Sharifimehrshahid bahonar universityManochehr Yosoufishahid bahonar universityMobin Aghapourshahid bahonar universityJournal Article20181108Anorexia is a part of the acute phase response (APR). Lipopolysaccharide (LPS) is frequently used to mimic APR and induces anorexia. The mechanism underlying anorexia associated with APR in chicks is not well understood. In the present study, the possible involvement of corticotrophin-releasing factor (CRF) on anorexic effects of LPS in neonatal chicks was investigated. For this aim, different doses of LPS were administrated via both intracerebroventricular (ICV) and intraperitoneal (IP) routes in order to assess its effects on chick’s food intake. Subsequently, the effect of ICV injection of astressin, a CRF receptor antagonist, on anorexia induced by ICV and IP administration of LPS was investigated. Food intake was significantly decreased following either central or systemic administration of LPS. ICV co -injection of astressin and LPS significantly diminished anorexic effects of central LPS. However, anorexia induced by peripheral LPS was not attenuated by central injection of astressin. These data indicated that the brain CRF receptors are involved in central LPS-induced anorexia in chicks.https://ijvst.um.ac.ir/article_30307_a21d0e33a032515685dc7a60bbfb05db.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701The eff ect of two Iranian viper snake; vipera albicornuta (zanjani) and vipera latifi i (lattifi i) venoms on the viability of rat bone marrow mesenchymal stem cells in vitro and in vivo27333033910.22067/veterinary.v1i11.76565ENFatemeh SalamiSchool of Veterinary MedicineFerdowsi University of Mashhad0000-0000-0000-0000Fatemeh Younesi SoltaniFaculty of Veterinary MedicineFerdowsi University of MashhadAmin TavassoliFaculty of Veterinary MedicineFerdowsi University of Mashhad0000-0001-7300-4369Behrooz FathiFaculty of Veterinary MedicineFerdowsi University of Mashhad0000-0003-2795-6443Journal Article20181112Snake venom is a complex mixture of different compounds which have potential pharmacological properties and may affect mesenchymal stem cells (MSCs). In this study we investigate the effects of two Iranian vipers; vipera albicornuta and vipera latifii crude venoms on the viability of MScs in vivo and in vitro. The cells in in vitro tests were treated with different concentrations (1, 2, 3, 4 and 5 μg/100μl) of mentioned venoms for 24, 48 and 72 hours.The cells in in vivo experiment only were treated with v. Latifii venom at the concentration of 1μg/100μl and time intervals as in vitro tests. The cell viability in in vitro experiment was assessed using MTT assay. The results of in vitro experiments showed that maximum cell viability was observed at concentrations of 1 and 2 μg/100μl of v. albicornuta and v. latifi ivenoms aft er 48 and 72 hours, respectively. The results of in vivo experiment showed that the cells treated with v. Latifii venom for 72 hours in situ have the highest proliferation rate after passages three, four and five in comparison to control. The results of this study showed that the v. albicornuta and v. latifi i venoms can affect the confluence and viability of the MSCs.https://ijvst.um.ac.ir/article_30339_5f19309a84029f7a179e59374b9ca299.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701Antibacterial eff ect of Lavandula stoechas and Origanum majorana essential oils against Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli35413037310.22067/veterinary.v1i11.77679ENSamira Nooriuniversity of gonbad kavous0000-0003-4301-0447Reza Rahchamaniuniversity of bonbad kavous0000-0001-5829-7757Javad Bayat Kohsaruniversity of gonbad kavousFatemeh Bahri Binabajuniversity of gonbad kavousJournal Article20190115This research examined the antimicrobial effect of Lavandula stoechas (lavender) and Origanum majorana (marjoram) essential oil against three mastitis-causing pathogens: Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. Gas chromatography-mass spectrometry (GC/MS) analysis revealed that the main components of the lavender and marjoram oils were 17-Pentatriacontene, Linalyl acetate, Eucalyptol, linalool and 3-Cyclohexene-1-ol,4-methyl-1-(1-methylethyl)-,(R)-, α-terpineol, P-cymene, respectively. Broth dilution testing was performed using autoclaved whole milk instead of broth to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of essential oils alone and in combination. In addition, time-kill assay of lavender and marjoram oils were determined in milk up to 24 h. MIC values ranged from 3.12 - 4.37% v/v and MBC between 6.25 - 8.75% v/v for the lavender. The MIC and MBC of the marjoram ranged from 0.62 - 1.87% v/v and 1.25 - 3.75% v/v, respectively. The MIC ranged from 2.5 - 5% v/v and MBC between 5 - 10% v/v for lavender + marjoram combination. In time-kill assays, the presence of lavender and marjoram oils at a sub-MIC concentration significantly reduced the bacterial population in 4, 10 and 24 h. Generally, essential oil of marjoram had greater antibacterial activity than lavender against all mastitis-causing pathogens tested and has the potential to be evaluated as an alternative or adjunct to antibiotics in the treatment of bovine mastitis.https://ijvst.um.ac.ir/article_30373_54fd8673a7a77fc7b41f63027e724e99.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701Effect of different levels of milkweed (Calotropis persica)seed powder on the growth parameters, immunity and gut microbiota of Oncorhynchus mykiss43503041310.22067/veterinary.v1i11.76088ENHamidreza Ahmadniaye MotlaghFerdowsi University of MashhadOmid SafariFerdowsi University of Mashhad0000-0001-8378-8291Marina PaolucciUniversity of SannioJournal Article20181022An 8-week feeding experiment was conducted to investigate the inclusion potential of five levels of Calotropis persica seed powder (CSP) (0, 10, 20, 30, 40 and 50 g/kg of the basal diet) in rainbow trout (Oncorhynchus mykiss) diet, in a completely randomized design. To perform the test, 3600 fries (11.5 ± 3.64 g) were treated for 56 days. According to the results, the increase in milkweed seed powder up to 40 g/kg, resulted in a significant increase in specific growth rate and intestinal lactic acid bacteria count compared to the control (p < 0.05). The best results of survival rate, feed conversion ratio, hepatosomatic and gastrosomatic indices were achieved in the treatments receiving 20, 30, 40, and 50 g/kg CSP (p < 0.05). Antibacterial activity of skin mucus, lysozyme and alkaline phosphatase showed the highest level in the 40 g/kg treatment (p < 0.05). Based on the results, the inclusion of 40 g/kg milkweed seed powder caused positive health effects and could be a suitable herbal feed additive in the rainbow trout diet.https://ijvst.um.ac.ir/article_30413_19215a3bcd5ebe2c66d9448bd4aa6dc0.pdfFerdowsi University of MashhadIranian Journal of Veterinary Science and Technology2008-465X11120190701The eff ects of extenders containing proline and glutamine on oxidative stress and motion parameters of stallion semen during cold storage51593044910.22067/veterinary.v11i1.80929ENNajmeh DavoodianShahrekord University of ShahrekordAli KadivarShahrekord University of Shahrekord0000-0002-3221-9635Ebrahim AhmadiShahrekord University of ShahrekordJournal Article20190525This study examined the effects of skim-milk based extenders supplemented with proline and glutamine on motility, lipid peroxidation and enzymatic antioxidant status of cooled-stored equine sperm and determined the role of seminal plasma as well. The semen was collected with artificial vagina. In experiment 1, native semen was diluted in skim-milk based extender containing 5mM glutamine and 3mM proline, stored at 5°C and analyzed at 4, 24 and 48 hours storage for motion parameters. In experiment 2, semen was centrifugated, sperm pellet resuspended in the extenders and stored at 5°C for 4 hours to determine motion parameters. The level of catalase, glutathione peroxidase activity and malondialdehyde formation was determined for all samples at 4 hours. Glutamine and proline signifi cantly preserved the percentage of motile sperm (76.5 ± 2.7 and 79.4 ± 1 vs 69 ± 1.4), increased the progressive motility of cold-stored semen (66.1 ± 2.5 and 73.7 ± 2.9 vs 56.2 ± 1.4), increased catalase activity, and decreased malondialdehyde. However these effects were disappeared after seminal plasma removal. We conclude that glutamine and proline would amplify the antioxidant activity of equine cold-stored semen and preserve its motility. This effect seems to be related to interactions with seminal plasma.https://ijvst.um.ac.ir/article_30449_9099215dfed8e3e4bf780c039d4a00cd.pdf