Ferdowsi University of Mashhad Iranian Journal of Veterinary Science and Technology 2008-465X 5 2 2013 12 01 Histopathological evaluation of the effect of platelet-rich fibrin on canine cutaneous incisional wound healing 19 32 26963 10.22067/veterinary.v5i2.18579 EN Majid Khanzadeh Alishahi Research Institute , Mashhad Davoud Kazemi Islamic Azad University, Tabriz 0000-0002-0684-2722 Darioush Mohajeri Islamic Azad University, Tabriz Hasan Mofidpoor Mashhad University of Medical Sciences Amir Afkhami Goli Ferdowsi University of Mashhad 0000-0002-3211-7620 Mohamad Ali Khanzadeh Alishahi Clinic of Dermatology, Mashhad Journal Article 2013 01 14 Platelets are a natural source of growth factors and cytokines that promote wound healing, and platelet-rich fibrin contains concentrated growth factors. Growth factors in platelet-rich fibrin may promote wound repair. In this study the effect of platelet-rich fibrin was evaluated in a dog model of cutaneous incisional wounds using histology and semi-quantitative evaluation. A pair of ten-centimeter full-thickness parallel linear cutaneous wounds were created on the backs of 15 dogs on the both sides of vertebral column. On the left side the platelet-rich fibrin clot was applied to the edges of the wound (Treatment group) and the right side received nothing (Control group). All wounds were then closed with 3-0 non-absorbable Nylon suture. The dogs were divided into three groups of five dogs. The wound tissues were sampled by electrosurgery in group one after 3 days, group two after 7 days and group three after 14 days post surgery. For each specimen, histopathological examination and semi-quantitative evaluation was performed by light microscopy using Hematoxylin & Eosin and Masson’s trichrome staining. The results demonstrated that platelet-rich fibrin improved and accelerated cutaneous incisional wound healing. https://ijvst.um.ac.ir/article_26963_2aad8aa7c3f8e4064e980e9528e50f53.pdf