Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Annexin A1, A2 and cytokine levels during experimental sepsis in calves
1
6
EN
Mohammad Reza
Mokhber Dezfouli
University of Tehran
mokhberd@ut.ac.ir
Zohre
Eftekhari
Research & Production Complex, Pasteur Institute of Iran, Tehran, Iran
eftekharivet@gmail.com
Masoomeh
Heidari Sureshjani
University of tehran
masoomeh_dvm@yahoo.com
Mohammad Mahdi
Dehghan
0000-0002-8825-9070
University of Tehran
mdehghan@ut.ac.ir
10.22067/veterinary.v9i1.54965
Annexins are fundamentally related proteins that process a variety of physiologic and pathologic procedures, including suppression of inflammation. Ten Holstein-Frisian bull calves (10 ± 1 days old) weighting 50 ± 5 kg were chosen to induce the experimental septicaemia using O111:H8 strain of E. coli. Blood samples were collected to determine the plasma annexin A1 , annexin A2, TNF-α, IFN- γ, IL-8 and neutrophil count at 0, 24, 48, 72, 96 and 120 hours after induction of septicemia. Significant increased concentrations of serum annexin A1 and annexin A2 in circulating blood in response to experimental coliseptisemia were observed during experiment. Maximum levels of annexinA1 and A2 were recorded at 72h after challenge. A statistically significant increase in <br />blood neutrophil count occurred from beginning of septicemia untill 24h after onset of septicemia. Annexin A2 and Annexin A1 had no significant correlation with neutrophil count. Serum cytokine concentrations reached their maximum level at 48h after challenge and then decreased to basal level before antibiotic therapy. This study showed that serum annexin concentrations, increasing during colisepticemia in calves, in association with cytokines could be a reliable marker to confirm the occurrence of anti-inflammatory response.
Annexin,calves,cytokines,E.coli
https://ijvst.um.ac.ir/article_28948.html
https://ijvst.um.ac.ir/article_28948_c16bce3601ce55481b2a20bf6a76f220.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Serological and molecular studies on visceral leishmaniosis in stray dogs in Torbat-e-Heidareih area
7
11
EN
Mohsen
Pourali
Ferdowsi University of Mashhad
poorali-m@mihanmail.ir
Gholamreza
Razmi
0000-0002-0754-1278
Ferdowsi University of Mashhad"
razmi@um.ac.ir
Sadaf
Sabzavari
North Khorasan of University of Medical Science
sadaf.sabzevari@yahoo.co
10.22067/veterinary.v9i1.59414
Visceral leishmaniosis is an important zoonotic disease in Iran. The agent, vector and reservoir are Leishmania infantum, Phlebetomous major and dogs, respectively. Thus far, no data has been reported about the prevalence of visceral leishmaniosis in human and dog in Torbat-e- Heidareah area and the present study has been performed to determine the frequency of L. infantum infection in stray dogs. One hundred blood and skin samples were collected from 2014 to 2015. The sera samples were examined by Indirect Immunofluorescent Antibody Test (IFAT). The skin samples of seropositive and seronegative in association with skin lesions were examined by PCR method. In this study, antibodies against L. infantum were detected in 3 (3%) sera samples of dogs and kDNA Leishmania spp. was detected in 4 (4%) of seronegative dogs by PCR. L. infantum was detected in positive samples by semi nested PCR. Based on serological and molecular results, 7% of asymptomatic stray dogs of Torbat-e- Heidareih area were infected with L. infantum and may be acted as reservoirs of visceral leishmaniosis.
Leishmania infantum,Stray dogs,Torbat-e- Heidareih area
https://ijvst.um.ac.ir/article_28980.html
https://ijvst.um.ac.ir/article_28980_48a19046592dad9d220869a11bf9def4.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
The investigation on the relationship between dairy cow hygiene scores and intramammary infections
12
16
EN
Iman
Rajabi
Ferdowsi University of Mashhad
iman_rajabi69@yahoo.com
Alireza
Taghavi Razavizadeh
0000-0000-0000-0000
Ferdowsi University of Mashhad
rezavizadeh@um.ac.ir
Mohammad
Azizzadeh
0000-0001-9192-5022
Ferdowsi University of Mashhad
m-azizzadeh@ferdowsi.um.ac.ir
babak
Khoramian toosi
0000000249622797
Ferdowsi University of Mashhad
khoramian@um.ac.ir
10.22067/veterinary.v9i1.57689
The “cow hygiene score” system is a management tool for assessment of cow’s body hygiene and cleaning of the barn. In this study, the correlation between body hygiene scores and intramammary infections (IMI), California Mastitis Test (CMT) and Somatic Cell Count (SCC) were evaluated in two different seasons. Hygiene scoring of 1096 dairy cows in 4 herds was performed on five body areas including: udder, rear legs, flanks and upper legs, abdomen, and tail head. After doing CMT, milk samples were taken from quarters with score 2 or more for microbiological culture. SCC data were taken in two consecutive months. The results of this study showed no significant differences between the median of all hygiene scores except for tail head that were significantly greater in high <br />than low rainy seasons (p < 0.05). There was no significant correlation between hygiene scores and the chance of positive bacterial culture (p > 0.05), but a statistically significant relationship was found between udder hygiene score and isolation of environmental bacteria (p < 0.05). There were no statistically significant differences between SCC or CMT and hygiene scores of all parts of the body and similarly between teat cleanliness score after premilking preparation and SCC in two consecutive months (p > 0.05). Finally, it seems that udder hygiene scoring is an useful tool for predicting of intramammary infections caused by environmental bacteria.
Hygiene Scores,intramammary infections,Dairy cow,California mastitis test,somatic cell count
https://ijvst.um.ac.ir/article_29030.html
https://ijvst.um.ac.ir/article_29030_c41742da9e2c545efd80a86b501012ba.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Isolation and molecular diagnosis of Peste des petits ruminants (PPR) virus from contaminated areas in Iran
17
21
EN
Roozbeh
Fallahi
0000-0001-8237-7618
Razi Vaccine and Serum Research Institute
r.fallahi@rvsri.ac.ir
10.22067/veterinary.v9i1.56792
Due to the numerous reports concerning the Peste des petits ruminants (PPR) in different regions of Iran, the isolation and genetic characterization of native isolates is very important. During 20132014, 168 samples were collected from whole blood, eye, nasal and oral swabs, and lymph nodes of sheep and goats with clinical signs in nine provinces with infected focal zones in Iran. Vero cell line and primary Lamb Kidney cells were inoculated with isolates and observed at least in 5 consecutive passages for cytopathic effects (CPE). The sheep samples from two provinces, created CPE in both kinds of cell cultures. Observation by electron microscopy and confirmation by RT-PCR was used to confirm PPR.
PPR,isolation,diagnosis,Iran
https://ijvst.um.ac.ir/article_29055.html
https://ijvst.um.ac.ir/article_29055_9c09b0c54546e7b66d3c94d1344f9deb.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Histomorphometric and ultrasonographic evaluations of the rumen in sheep
22
27
EN
Mohammad
Movahedi Zade
Ferdowsi University of Mashhad
reshkoh68@yahoo.com
Ahmad Reza
Raji
0000-0002-8192-8374
Ferdowsi University of Mashhad
rajireza@um.ac.ir
Ali
Mirshahi
0000-0001-6372-6931
Ferdowsi University of Mashhad
mirshahi@um.ac.ir
10.22067/veterinary.v9i1.58968
Rumen lesion can lower the performance of the animal and sometimes cause its death. Ultrasonography as a diagnostic method for the detection of lesions in the gastrointestinal tract is considered safe. In this study, three regions of rumen including the dorsal blind sac, ventral blind sac, and pillar [0.5 × 0.5 cm] from 10 healthy sheep rumen were sampled. Histomorphometric study of all samples were performed in the mucosal, submucosal, muscular and serosal layers. For ultrasonographic evaluation, samples from wall of rumen in 6 × 6 cm dimensions were used probe. The results showed that identification of all layers of rumen wall is feasible in sheep by histomophometry and ultrasonography techniques. Statistical analysis of the data showed no significant correlation between the <br />parameters of the rumen wall in ultrasonography and histological study. The lack of correlation between ultrasonography and histological data may be due to the tissue changes which would occur during the process of preparing the tissue samples including tissue fixation, dehydration and clearing
Ultrasonography,histomorphometry,rumen,Sheep
https://ijvst.um.ac.ir/article_29111.html
https://ijvst.um.ac.ir/article_29111_be890df47c75a39cfdb9f54e3ab8f397.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Histomorphogenesis of pancreas in ostrich embryo (Struthio camelus)
28
32
EN
Masoumeh
Ahadian
Ferdowsi University
jul_25mo@yahoo.com
Zohreh
Saadatfar
Ferdowsi University of Mashhad
saadatfar@ferdowsi.um.ac.ir
Hamideh
Ghodrati Azadi
Ferdowsi university
ghodrati@um.ac.ir
10.22067/veterinary.v9i1.61068
The purpose of this study was evolution of pancreas in ostrich. The study was done on 24 incubated eggs of ostrich embryo on 10, 13, 16, 22, 26, 30 and 36 days of prehatching and 1 day of post hatching life. The histological stains such as haematoxylin eosin (H&E), gomori and Toluidine blue were used for this study. On 13th day of incubation the primary pancreas was developed near the liver and duodenum and it was composed of one part. The pancreas was consisted of undifferentiated epithelial cells, connective tissue, non -organized ducts and blood vessels. On 16th day the pancreas was composed of dorsal and ventral lobe with the similar structure to 13th day. On the 22th day, the zymogen granules were evident in developing exocrine part. By aging the level of connective tissue and ducts were decreased and the acinar cells were increased. On 1th day after hatching the hepatocytes vacuolated severely and pancreatic islets were appeared.
Ostrich,histology,pancreas,development
https://ijvst.um.ac.ir/article_29150.html
https://ijvst.um.ac.ir/article_29150_8a63e6d53bb811037b25f284127a56fa.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Effect of sex on histological and histochemical structures of interdigital sinus in adult Bakhtiari sheep of Iran
33
38
EN
Behzad
Mobini
0000-0001-9703-014X
Islamic Azad University, Shahrekord Branch
dr.mobini@iaushk.ac.ir
Vahid
Ranjkesh Adermanabadi
Islamic Azad UniversityU Shahrekord Branch
ranjkeshvahid@yahoo.com
10.22067/veterinary.v9i1.59621
The aim of this study was to investigate the effect of gender on the histological and histochemical structures of various anatomical regions of the interdigital sinus in the Iranian native sheep. Tissue samples from different anatomical regions of each sinus were obtained from 15 female and 15 male adult Bakhtiari sheep. The sections stained with H&E, Periodic acid- Schiff, Alcian blue, Verhoeff’s, Masson’s trichrome, and Gomori’s method. The sinus was covered by skin and fibrous capsule. The sinus contained descending part, flexure, ascending part, excretory duct and orifice. The sebaceous glands were simple branched acinar and the sweat glands were simple coiled tubular type. The secretory caps were observed in the secretory cells protruded into the lumen. The various histological and histochemical structures of the interdigital sinus showed no considerable differences among various anatomical regions of the right and left sinuses. Also, no significant sex-based differences were found. The surface epithelial cells of the apocrine glands and their secretion contained both neutral and acidic glycosaminoglycans. It is concluded that the general histological and histochemical structures of the interdigital sinus in Bakhtiari sheep were similar to those of other native sheep, but there were also some differences.
Histochemical,histology,interdigital sinus,sex,Sheep
https://ijvst.um.ac.ir/article_29178.html
https://ijvst.um.ac.ir/article_29178_01315cf34240c94586b213c3bb2ae1e3.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Effect of thiamine and vitamin C on tissue lead accumulation following experimental lead poisoning in Cyprinus carpio
39
44
EN
Davar
Shahsavani
0000-0002-5779-2569
Ferdowsi University of Mashhad
shahsavani@um.ac.ir
Hassan
Baghishani
1111-1111-1111-1111
Ferdowsi University of Mashhad"
baghishani@um.ac.ir
Kimiya
Nourian
Ferdowsi University of Mashhad
ki_no78@stu.um.ac.ir
10.22067/veterinary.v9i1.53864
The present study was conducted to evaluate the possible ameliorative effects of vitamin C and thiamine on lead accumulation in kidney, liver, muscle, brain and gill of experimentally lead-poisoned common carp. At the beginning of the experiment, fish (n=120) were divided into 4 groups randomly with group 1 being considered as the control group. Groups 2, 3 and 4 were exposed to lead acetate (5 mg/L, 15 days); groups 3 and 4 received vitamin C (500 mg/kg feed) and thiamine (50 mg/kg feed) during lead acetate exposure, respectively. Following this, it was observed that lead exposure caused a significant (p < 0.05) increase in lead content in all examined tissues of fish in group 2 in comparison to control group. It was also found that thiamine supplementation slight<br />ly decreased the augmented levels of lead in the muscle, brain and gill tissues, which was not significantly different from that of the control group. Similarly, vitamin C supplementation reduced the augmented concentrations of lead in the muscle to the levels that were not significantly different from that of the control group. Based on the present results, neither thiamine nor vitamin C was effective in providing a significant reduction of tissue lead burden in groups 3 and 4 as compared to group 2. Thus, monotherapy with such vitamins cannot be proposed as a suitable therapeutic approach for the effective reduction of the tissue lead burden in common carp. However, further investigations using other dosing regimens of each vitamin or combined treatment with chelators are required to reach such a conclusion.
Lead,tissue,common carp,thiamine,vitamin C
https://ijvst.um.ac.ir/article_29209.html
https://ijvst.um.ac.ir/article_29209_58a2c158abd0577f307661b37cdbf6d7.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Fibrosarcoma in a Goldfish (Carassius auratus): a Case report
45
48
EN
Annahita
Rezaie
Shahid Chamran University of ahvaz
a.rezaie@scu.ac.ir
Zahra
Tulaby Dezfuly
Shahid Chamran university of ahvaz
z.tulaby@gmail.com
Rahim
Peyghan
0000-0002-2416-7230
Shahid Chamran university of ahvaz
peyghan_r@scu.ac.ir
10.22067/veterinary.v9i1.58619
Neoplasms in fishes are generally less aggressive than neoplasms in mammals and are most commonly discrete and focal. Although tumors were originated from connective tissue are frequently seen in the fishes, but dermal fibrosarcoma is rarely found in fish. A moribund four years old Goldfish (Carassius auratus), was referred veterinary hospital of Shahid Chamran University of Ahvaz with an exophytic non-ulcerated mass near the caudal peduncle. Microscopic examination of the mass revealed proliferated spindle cells and the interlacing, loose and eosinophilic bundles. For differential diagnosis, Masson’s trichrome staining was performed and the fibers stained blue. According to histopathological and histochemical results, tumor identified as fibrosarcoma. It is the first report of histopathological features of cutaneous fibrosarcomas in a Goldfish in Iran.
Fibrosarcoma,goldfish
https://ijvst.um.ac.ir/article_29014.html
https://ijvst.um.ac.ir/article_29014_21339846861f6ff759a20d765b283f27.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
9
1
2017
09
01
Histopathologic report of infestation by Centrocestus formosanus in Iranian grass carp and common carp
49
53
EN
Annahita
Rezaie
Shahid Chamran University of ahvaz
a.rezaie@scu.ac.ir
zahra
Tulaby Dezfuly
shahid chamran university of ahvaz
z.tulaby@gmail.com
Mehrzad
Mesbah
shahid chamran university of ahvaz
m.mesbah@scu.ac.ir
Amin
Ranjbar
shahid chamran university of ahvaz
a.ranjbar@scu.ac.ir
10.22067/veterinary.v9i1.62662
Flukes cause severe and lethal diseases in various animals comprising fish. Both adult and larval stages are found in fish. Centrocestiasis is an infection of the fish gills by heterophyid trematode Centrocestus formosanus. In summer 2014, 35 fingerling grass carp and 30 common carp weight of 6 grams were referred to the veterinary hospital of Shahid Chamran University of Ahvaz. In the wet mount of the skin, mild infection of trichodina was observed. Wet mount of grass carp gills revealed large number of parasitic cysts between gill filaments. The cysts were quite clear and contained pear-shaped parasites. In histopathological examination, filaments of gill were thick and distorted. According to the wet mount and histopathologic results, metacercariae was chracterised to belong to heterophyidae, C. formosanus.
Centrocestus formosanus,grass carp,common carp,histopathology
https://ijvst.um.ac.ir/article_29076.html
https://ijvst.um.ac.ir/article_29076_7a1fe57a9da62457c27e30c34ec16dde.pdf