Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Therapeutic effects of pomegranate (Punica granatum) pith and carpellary membrane extract on lead-induced toxicity in rats
1
10
EN
Mohammad-Reza
Aslani
0000-0001-5479-676X
University of Shahrekord
mraslani@ferdowsi.um.ac.ir
Mahboobe
Azad
Urmia University
Mehrdad
Mohri
0000000337568890
Ferdowsi University of Mashhad
mohri@um.ac.ir
Vahid
Najarnezhad
0000-0002-7187-6515
University of Urmia
v.najarnezhad@urmia.ac.ir
10.22067/veterinary.v6i2.32177
Pomegranate is an ancient edible fruit with various pharmaceutical bioactivities such as antioxidant, lipid regulation and anti-inflammation. In this study the effectiveness of pomegranate pith and carpellary membrane (PPCM) extract in treatment of experimental lead intoxication was assessed in rats. Female Wistar rats were exposed to 1000ppm lead acetate in drinking water for 35 days and treated thereafter with PPCM extiract (100 and 200mg/kg, orally) twice a day for 35 days. The concentration of lead in blood, kidney, liver, bone and brain were measured using atomic absorption spectrophotometry. Treatment with PPCM extract reduced lead retention in blood and tissues. With the highest dose of PPCM extract, the greatest rate of reduction of lead concentrations were observed in brain (61%), blood (53%), and bone (34.5%). No significant changes were observed in copper, zinc and iron concentration of serum and liver, in neither doses of PPCM extract. In conclusion it was demonestrated that PPCM and carpellary membrane had therapeutic effect in the treatment of lead intoxication without any side effects on essential elements in blood and tissues of rats.
punica granatum,lead intoxication,Microelements,Rat
https://ijvst.um.ac.ir/article_27327.html
https://ijvst.um.ac.ir/article_27327_e1cb8658469ba6d3e2b7d9587941c515.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Effects of Gonadotropin Releasing Hormone (GnRH) on bovine spermatogonial stem cell proliferationatogonial stem cells proliferation
11
20
EN
Parviz
Tajik
Islamic Azad University
ptajik@ut.ac.ir
Hesam
Kohsari
Islamic Azad University
kohsari.hesam@yahoo.com
Babak
Qasemi-Panahi
University of Tabriz
babakpanahi98@gmail.com
Iraj
Sohrabi-Haghdoost
Science and Research Branch, Islamic Azad University
sohrabi@yahoo.com
Abbas
Barin
University of Tehran
abarin05@yahoo.com
Hamid
Ghasemzadeh-Nava
University of Tehran
ghasemzadeh@ut.ac.ir
10.22067/veterinary.v6i2.21007
Identification of exogenous factors affecting spermatogonial stem cells (SSCs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. The aim of this study was to investigate the effects of a GnRH analogue (alareline acetate) on SSCs colonization in short-term co-culture with sertoli cells. Five, three-month old Holstein male calves were used to isolate spermatogonial and sertoli cells. Testicular germ cell collection was made by enzymatic digestion methods. The cells were co-cultured in a 15 day period and in vitro effects of various doses (0.5, 1, 2 and 4 µg/ml) of GnRHa on SSCs colonization were assessed. Effects of GnRHa on SSCs proliferation were dose dependent. In conclusion, it was demonstrated that 1 µg/ml GnRHa was the optimum dose for SSCs colonization in comparison with control group. The highest treatment dose (4 µg/ml GnRHa), negatively affected SSCs colonization in comparison with control group.
GnRH,Calf,Spermatogonial Stem Cell,Sertoli cell,Co-culture
https://ijvst.um.ac.ir/article_27353.html
https://ijvst.um.ac.ir/article_27353_f8f552b510067e688c5d6ec600bb00b7.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Detection of Coxiella burnetii and sequencing the IS1111 gene fragment in bulk tank milk of dairy herds
21
28
EN
shiva
Borji
Ferdowsi University of Mashhad
shiva_borji84@yahoo.com
abdollah
Jamshidi
0000-0001-9935-4666
Ferdowsi University of Mashhad
ajamshid@um.ac.ir
saeid
khanzadi
Ferdowsi University of Mashhad
khanzaadi@um.ac.ir
Jamshid
Razmyar
0000-0002-1247-4591
Ferdowsi University of Mashhad
jrazmyar@alumni.ut.ac.ir
10.22067/veterinary.v6i2.26330
Coxiella burnetii is the etiologic agent of Q fever in human. This study is aimed to determine the contamination rate of C. burnetii in bulk tank milk samples. In this study, a total number of 100 bulk milk samples collected from dairy farms in northeast of Iran and were examined for C. burnetii using touchdown PCR assay. The primers were designed to amplify a 687-bp fragment of the IS1111 gene of C. burnetii. Five samples (5%) of bovine milk were found positive for C. burnetii. Sequence analysis of PCR products revealed 100% identity with published sequences of C. burnetii in genBank. Results of this study indicate that bovine milk is an important source of C. burnetii infection in Iran.
Bulk tank milk,Coxiella burnetii,Touchdown PCR
https://ijvst.um.ac.ir/article_27383.html
https://ijvst.um.ac.ir/article_27383_1f2652edef44fc0bb6b3acacf5b1c038.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Evaluation of histopathological features of Argas reflexus bite in pigeon
29
41
EN
Maryam
Gholizadeh
Urmia University
gholizadehmaryam60@yahoo.com
Mousa
Tavassoli
0000-0002-0169--6041
Urmia University
m.tavassoli@urmia.ac.ir
Farid
Rezaei
Razi University
f.rezaei@razi.ac.ir
Zahra
Nikousefat
Razi University
nikousefatz@razi.ac.ir
10.22067/veterinary.v6i2.21042
Argas reflexus is considered obligate blood feeder that may cause skin redness and local swelling. For histopathological evaluation of biting site, pigeons were used as natural host species in this study. Adult ticks were collected from Urmia, North-west of Iran and kept in a desiccator before their use in the experiment. Sixteen Pigeons were infested experimentally and were divided into 4 groups, euthanized after 30 min, 4, 24 and 48h for evaluation of histopathological changes in biting sites. Early histopathologic lesions due to time of infestation included edema, hemorrhagic foci, vascular congestion, necrosis and mild to severe inflammatory reaction in dermis. In late lesions, presence of micro abscess foci in dermis was prominent. This study well documented the different pathological changes of skin of pigeons with Argas reflexus. However, further study should be performed to evaluate allergic immune responses and pathological changes in other animal species including human.
Argas reflexus,Tick,Pigeon,histopathology
https://ijvst.um.ac.ir/article_27424.html
https://ijvst.um.ac.ir/article_27424_2cbf4526e099bd3c9e29bcaac6cbe6e6.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Tert-butylhydroquinone (TBHQ) improves antioxidant status in rat tissues following chronic diazinon intoxication
42
52
EN
saman
Sargazi
Ferdowsi University of Mashhad
saman.sargazi@gmail.com
Amir
Moghadam Jafari
Ferdowsi University of Mashhad
moghaddamjafari@um.ac.ir
Mohammad
Heydarpour
0000000205807984
Ferdowsi University of Mashhad
heidarpour@um.ac.ir
10.22067/veterinary.v6i2.39503
Considering that the involvement of oxidative stress has been implicated in the toxicity of organophosphate insecticides, the objective of the present study was to investigate antioxidants and oxidative stress markers in the liver and kidney of Wistar rats treated with chronic doses of diazinon. In addition, the effect of Tert-butylhydroquinone (TBHQ), a food-additive antioxidant, on attenuation of diazinon-induced oxidative stress was evaluated. 28 rats were randomly assigned to 1 of 4 treatment groups: diazinon (10 mg/kg BW, once a day; n=7), TBHQ (0.028 g/kg of diet, once a day; n=7), TBHQ + diazinon (diazinon; 10 mg/kg BW, once a day + TBHQ; 0.028 g/kg of diet, once a day; n=7) and control (corn oil, as vehicle of diazinon and TBHQ, n=7). TBHQ, diazinon and corn oil were given to rats orally via gavage for 7 weeks. Total thiol groups, ferric reducing antioxidant power (FRAP) and malondialdehyde (MDA) levels in liver and kidney tissues were investigated at the end of 7th week. Treatment with diazinon significantly increased MDA level, as a marker of lipid peroxidation, in the kidney (p
diazinon,oxidative stress, TBHQ,Rat
https://ijvst.um.ac.ir/article_27455.html
https://ijvst.um.ac.ir/article_27455_6f5ba5f5b5e845c4c12da2ad66cfc889.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Serum concentrations of lipids and lipoproteins and their correlations with thyroid hormones in clinically healthy German shepherd dogs: Effects of season, sex and age
53
61
EN
Bahman
Mosallanejad
0000-0001-9150-7920
Shahid Chamran University of Ahvaz
bmosallanejad@scu.ac.ir
Ali Reza
Ghadiri
Shahid Chamran University of Ahvaz
Reza
Avizeh
Shahid Chamran University of Ahvaz
avizeh@scu.ac.ir
Mahdi
Pourmahdi
Shahid Chamran University of Ahvaz
Marziae
Rajabalipour
Shahid Chamran University of Ahvaz
10.22067/veterinary.v6i2.22722
Although there are many evidences regard to the relationship between serum thyroid hormones, cholesterol, triglyceride and lipoproteins in different animals, but there are limited references in dogs. The aim of this study was to determine the correlation between thyroid hormones and concentrations of serum lipid and lipoprotein profiles, and also to detect relationship between the measured parameters in different season, sex and age groups of dogs in Ahvaz district, Southwest of Iran, on 2011. Blood samples were taken from the cephalic vein of 60 clinically healthy and non-pregnant native dogs in two seasons (summer and winter) and in two age groups (3 years). Sampling was done only once from each animal (30 dogs in every season). The serum concentrations of cholesterol, triglyceride (T.G), very low density lipoproteins (VLDL-cholesterol), low density lipoproteins (LDL-cholesterol), high density lipoproteins (HDL-cholesterol and their correlations with thyroxine (T4) and triiodothyronine (T3) hormones were measured in the studied dogs. The mean serum concentrations of cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol (mmol/L), T4 and T3 (µmol/L) were measured in the present survey. The above parameters were also obtained in two summer and winter seasons. Season had a significant effect on T.G, HDL, LDL and VLDL levels in the studied dogs (p0.05). No significant differences were detected for the measured cholesterol, T3 and T4 parameters in different age, sex and season groups (p>0.05). To the best of our knowledge, there was no previous research regarding the correlation of the thyroid hormones with the serum lipids and lipoproteins in dogs in Iran. Our results showed that thyroid hormones affect T.G, HDL, LDL and VLDL levels and observed seasonal change in serum may be partly due to changes in thyroid hormones.
thyroxine (T4),triiodothyronine (T3), lipid,lipoprotein,Dog,Ahvaz
https://ijvst.um.ac.ir/article_27478.html
https://ijvst.um.ac.ir/article_27478_6f44bb986ed427a52873fec1602fb45a.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Is inflammatory pulpal pain a risk factor for amnesia?
62
76
EN
Maryam
Raoof
Kerman University of Medical Sciences
m.raoof@acta.nl
Ramin
Raoof
Kerman University of Medical Sciences
raoof_ramin@yahoo.com
Mehdi
Abbasnejad
0000-0003-2570-5288
Shahid Bahonar University
mabbas@uk.ac.ir
Saeed
Esmaeili Mahani
0000-0001-5550-9806
Shahid Bahonar University
semahani@uk.ac.ir
Mohsen
Ramezani
Mazandaran University of Medical Sciences
Hamed
Ebrahim nejad
Mashhad University of Medical Sciences
ebrahimnh911@mums.ac.ir
Sara
Amanpour
0000-0003-4803-2274
Kerman University of Medical Sciences
saraamanpour@gmail.com
Jahangir
Haghani
Kerman University of Medical Sciences
haghanijahangir@gmail.com
10.22067/veterinary.v6i2.33591
The aim of this study was to investigate the effect of inflammatory pulpal pain on spatial learning and memory in male Wistar rats. Fifty-six adult rats were divided into eight groups as follows (n=7 per group): control, sham-operated group that received normal saline, sham vehicle group that received vehicle of capsaicin, three capsaicin treated groups that received intradental injection of 10, 25 and 100 μg capsaicin, respectively, formalin treated group that received 10 μl formalin 2.5% and ibuprofen treated group that received ibuprofen 20 min before capsaicin (100 μg) injection. After preparing cavities via cutting 2 mm of the distal extremities of the mandibular incisors, the polyethylene crowns were placed on the teeth. Based on the study group, different algesics were administrated under the crowns. After recording the pain scores, spatial learning and memory was assessed using Morris water maze test. Capsaicin 25, 100 μg and formalin 2.5% applications induced significantly more painful stimulation compared with control groups (p< 0.001). Capsaicin 25, 100 μg and also formalin-treated groups significantly showed increased escape latency and traveled distance (p
odontalgia, capsaicin,formalin,spatial learning and memory,morris water maze
https://ijvst.um.ac.ir/article_27512.html
https://ijvst.um.ac.ir/article_27512_0189feef1c54540af8215d53ec18fc77.pdf
Ferdowsi University of Mashhad
Iranian Journal of Veterinary Science and Technology
2008-465X
2423-6306
6
2
2014
01
01
Osteoarthritis and systemic infection caused by Candida Albicans in a common Mynah (Acridotheres tristis)
77
84
EN
Jamshid
Razmyar
0000-0002-1247-4591
Ferdowsi University of Mashhad
jrazmyar@alumni.ut.ac.ir
Ahmadreza
Movassaghi
Ferdowsi University of Mashhad
armovassaghi@yahoo.com
Ali
Mirshahi
0000-0001-6372-6931
Ferdowsi University of Mashhad
a.mirshahi@um.ac.ir
Samaneh
Eidi
0000-0002-6433-702X
Ferdowsi University of Mashhad
eidi@um.ac.ir
Mahdieh
Zaeemi
Ferdowsi University of Mashhad
zaeemi@um.ac.ir
Massoud
Rezaee
0000-0002-0111-7871
Ferdowsi University of Mashhad
massoud.rezaee@yahoo.com
10.22067/veterinary.v6i2.27859
A 2-year-old male Mynah (Acridotheres tristis) was referred with history of abdominal hernia surgery, depression, cachexia, lethargy and swelling of foot joints. Hematological and biochemical findings consisted of leukocytosis, heterophilia, monocytosis, elevation of liver enzymes activity, hyperproteinemia and hyperglobulinemia. Fine needle aspiration (FNA) was performed from foot joints, aseptically. Mycological and bacteriological evaluations were shown Candida albicans infection. In spite of local and systemic treatments, signs were deteriorated and the animal was euthanized and then necropsy was done. Pathological findings revealed systemic candidiasis and fungal osteoarthritis.
Candida albicans,osteoarthritis,Mynah (Acridotheres tristis),systemic candidiasis
https://ijvst.um.ac.ir/article_27276.html
https://ijvst.um.ac.ir/article_27276_a3e015d19f73137ada1c2dd68e23cf00.pdf