ORIGINAL_ARTICLE
Prevalence of canine cutaneous neoplasms from Shiraz, Iran
The present study was conducted on 42 dogs with a histopathological diagnosis of skin neoplasia presented in the Shiraz University Veterinary Clinic from April 2012 to December 2017. All cases were reviewed, excluding the mammary gland neoplasms. The histopathological type, prevalence, sex, age, breed and site distribution of the neoplasms were described. In addition, previous studies on canine skin tumors from other geographic regions were evaluated and compared with the results of the present study. Fifteen different histopathological types of tumor were diagnosed. The prevalence of epithelial, mesenchymal, melanocytic and lymphohistiocytic tumors was 61.9%, 35.7%, 2.4% and 0%, respectively. Th e three most common tumors were sebaceous gland adenoma (21.42%), squamous cell carcinoma (11.9%), and lipoma (11.9%). The incidences of these tumors were more than other researches. Although there is no obvious explanation for these geographical differences, the possible reasons may be the geographical locations, environmental infl uences, and the study population and breed. Terriers were the most common type of the dogs in this study (34.4%). The present findings about the dogs age and various skin tumors and the anatomical locations indicates that there is no signifi cant variation in these important parameters among the Iranian dogs and dogs from other parts of the world.
https://ijvst.um.ac.ir/article_30211_278d3ecd1fc85f6b2b68f826f8e3e704.pdf
2019-07-01
1
6
10.22067/veterinary.v1i11.79564
Cutaneous neoplasms
Dog
histopathology
Iran
Mohammad
Abbaszadeh Hasiri
vetmail@gmail.com
1
Shiraz University
AUTHOR
Fatemeh
Namazi
fnamazi@shirazu.ac.ir
2
Shiraz University
LEAD_AUTHOR
Forough
Zarei kordshouli
forugh_zarei@yahoo.com
3
Shiraz University
AUTHOR
1. Anudep R, Boonmee S, Lek O, Sailasuta A, Th anawongnuwech
1
R and Teankum K. Neoplasms of dogs in Bangkok.
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Th ai. J. Vet. Med. 2003 33(1): 60–66.
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2. Murphy S. Skin neoplasia in small animals. Common canine
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tumours. In Practice. 2006; 28(7): 398–402.
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3. Graf R, Pospischil A, Guscetti F, Meier D, Welle M and Dettwiler
6
M. Cutaneous Tumors in Swiss Dogs: Retrospective
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Data From the Swiss Canine Cancer Registry, 2008–2013. Vet.
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Pathol. 2018; DOI: 10.1177/0300985818789466.
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4. Bostock DE. Neoplasms of the skin and subcutaneous tissues
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in dogs and cats. Brit. Vet. J. 1986; 142: 1–19.
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MN and Karayannopoulou M. Prevalence, distribution and
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of cutaneous neoplasms in 174 dogs admitted to a
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clinic in northern Greece. J. Vet. Med. 2002; 49(2): 87–91.
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7. Chikweto A, McNeil P, Bhaiyat MI, Stone D and Sharma
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RN. Neoplastic and Nonneoplastic Cutaneous Tumors
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of Dogs in Grenada, West Indies. ISRN Vet. Sci. 2011;
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DOI:10.5402/2011/416435: 1–6.
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8. Mukaratirwa S, Chipunza J, Chitanga S, Chimonyo M and
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Bhebhe E. Canine cutaneous neoplasms: prevalence and infl
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uence of age, sex and site on the presence and potential malignancy
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Afr. Vet. Assoc. 2005; 76(2): 59–62.
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JH, Yoon BI, Choi YK and Kim DY. Retrospective study of
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12. De Souza TM, Fighera RA, Irigoyen LF and De Barros CSL.
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Retrospective study on 761 canine skin tumors. Cienc. Rural.
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2006; 36(2): 555–560.
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14. Nikula KJ, Benjamin SA, Angleton
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GM, Saunders WJ and Lee AC. Ultraviolet
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radiation, solar dermatosis, and
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Radiat. Res. 1992; 129(1): 11-18.
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neoplasms in dogs from the Brisbane
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region. Aust. Vet. J. 1989; 66(7): 225–
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16. Vail DM and Withrow SJ. Tumors of
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Withrow SJ, MacEwen EG, editors.
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ed. Philadelphia, Saunders; 2001; P:
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Tumors of the skin and soft tissues. In:
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Meuten DJ, editor. Tumors in Domestic
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Ames; 2002; P: 45-117.
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LAUFERSWEILER VERLAG, Giessen,
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Germany; 2006.
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AA, Von Tscharner C, Walder EJ
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and Yager JA. Histological classifi cation
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of epithelial and melanocytic tumors
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of the skin of domestic animals.
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ORIGINAL_ARTICLE
Experimental infection of pheasants with a velogenic chicken isolate of Newcastle disease virus
Newcastle disease (ND) is a highly contagious infection of many avian species, causing enormous losses in poultry production worldwide. The objective of this study was to reveal the clinical feature, virus shedding, and immune response following infection with a velogenic chicken isolate of Newcastle disease virus (NDV) in susceptible and vaccinated pheasants. Eighty day-old pheasant chicks were allotted to four groups. At 30 days of age, the birds in groups 1 and 3 were vaccinated with B1 strain via eye drop. Two weeks later, each bird in groups 1 and 2 was inoculated with 100 μL(50 μL/eye) of NDV-infected allantoic fluid containing 105 EID50 of viral inoculum. All groups were inspected daily for three weeks. Swab samples were taken at different time points and verifi ed for NDV infection by using reverse-transcription polymerase chain reaction (RT-PCR). Serological examination was also made by haemagglutination-inhibition assay. Clinically, watery mucoid feces was observed only in one case among the vaccinated challenged birds, whereas the unvaccinated challenged birds showed anorexia, mild depression and head deviation. Out of 20 birds in group 2, one case (5%) died. Based on RT-PCR, virus shedding was only observed among the unvaccinated birds from 5 to 14 days aft er challenge. The NDV was detected more in tracheal swabs (40%) than in cloacal swabs (30%). The infected birds showed a high seroconversion. In conclusion, the velogenic NDV circulating in Iranian chicken flocks has a low pathogenicity for pheasants, and ocular vaccination with B1 strain could provide a good protection.
https://ijvst.um.ac.ir/article_30244_fbd4298595164b68fafcec9d040eb2fa.pdf
2019-07-01
7
12
10.22067/veterinary.v1i11.80546
Immune response
Newcastle Disease
Pheasant
Virus shedding
Ramezan Ali
Jafari
jafari.ramezanali@scu.ac.ir
1
Shahid Chamran University of Ahvaz
LEAD_AUTHOR
Anahita
Rezaie
a.rezaie@scu.ac.ir
2
Shahid Chamran University of
AUTHOR
Zahra
Boroomand
z.boroomand@scu.ac.ir
3
Shahid Chamran University
AUTHOR
Mansoor
Mayahi
mansoormayahi@scu.ac.ir
4
Shahid Chamran University
AUTHOR
Reza
Zare
reza.zare90@yahoo.com
5
Shahid Chamran University
AUTHOR
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(OIE). Newcastle disease. In: Manual
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3. Miller PJ, Koch G. Newcastle disease.
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In: Swayne DE, Glisson JR, McDougald
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LR, Nolan LK, Saurez DL, Nair
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V, editors. Diseases of poultry. 13th
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5. Gowthaman V, Singh SD, Barathidasan
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R, Ayanur A, Dhama K. Natural outbreak
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with chicken in a multi–species poultry
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2009 and 2010. Avian Pathol.
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farms during 2009-2011. Vet Res
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Agustini M, Ventura HLB, Junior LD.
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McFarland M, Hietala SK, Velez V, et
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al. Th e isolation of exotic Newcastle
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California: 2002–2003. Avian Dis.
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2005;49(2):195-8.
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20. Jafari RA, Boroomand Z, Rezaie A,
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Mayahi M, Nejati Saravi A. Experimental
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disease virus from fi eld outbreaks in
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broiler and layer chickens by reverse
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CL, Purvis LB. Th e VG/GA strain of
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23. Piacenti AM, King DJ, Seal BS, Zhang
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J, Brown CC. Pathogenesis of Newcastle
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24. Alexander DJ, Senne DA. Newcastle
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disease. In: Saif YM, Fadly AM, Glisson
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ORIGINAL_ARTICLE
The Eff ect of resistance training and growth hormone injection on circulating IGF-1 and IGFBP-3 levels in a rat model
Growth hormone has mitotic and anti-apoptotic effects which may increase proliferation and transformation of cells when it is expressed aberrantly. This study investigated the effects of resistance training and growth hormone injection on circulating IGF-1, IGFBP-3 levels and IGF-1/IGFBP-3 ratio in male Wistar rats. Thirty-two male Wistar rats were randomly assigned to a control group (C, n = 8), a resistance training group (RT, n = 8), a growth hormone injection group (GI, n = 8) and a resistance training + growth hormone injection group (RG, n = 8). The resistance training protocol comprised of climbing a ladder (5 days/week, 3 sets/5 reps) while carrying a weight suspended from the tail. The growth hormone (2 mg/kg/day, 5 days/week) was injected before an exercise session. Serum IGF-1, IGFBP-3 levels, and IGF-1/IGFBP-3 ratio were measured after 8 weeks. One-way ANOVA analysis was used for comparison of serum IGF-1 and IGFBP-3 levels between groups. Serum IGF-1 levels and IGF-1/IGFBP-3 ratio signifi cantly decreased, but serum IGFBP-3 levels showed no significant change in the RT group compared to the C group. Also, both serum IGF-1 and IGFBP-3 levels and IGF-1/IGFBP-3 ratio in GI and RG groups significantly increased compared to the other groups. In conclusion, resistance training decreases serum IGF-1 levels and/or IGF-1/IGFBP-3 ratio in normal condition. On the other hand, the growth hormone injection with and without the resistance training increases serum IGF-1 levels and IGF-1/IGFBP-3 ratio which could be noted as a condition with a higher risk of neoplasm.
https://ijvst.um.ac.ir/article_30264_82ba9e85d0b0abe787c9adfaabf8a0c1.pdf
2019-07-01
13
18
10.22067/veterinary.v1i11.78504
Resistance training
Growth hormone injection
IGF-1
IGFBP-3
cancer
Behnam
Roozbeh
behnam.roozbeh@mail.um.ac.ir
1
Ferdowsi University of Mashhad
AUTHOR
Mahtab
Moazami
moazami@um.ac.ir
2
Ferdowsi University of Mashhad
LEAD_AUTHOR
Amir
Rashidlamir
rashidlamir@um.ac.ir
3
Ferdowsi University of Mashhad
AUTHOR
Zahra
Moosavi
zmoosavi@um.ac.ir
4
Ferdowsi University of Mashhad
AUTHOR
Ali
Javadmanesh
javadmanesh@um.ac.ir
5
Ferdowsi University of Mashhad
AUTHOR
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ORIGINAL_ARTICLE
Interactive effects of peripheral and central administration of LPS with inhibition of CRF receptors on food intake in neonatal chicks
Anorexia is a part of the acute phase response (APR). Lipopolysaccharide (LPS) is frequently used to mimic APR and induces anorexia. The mechanism underlying anorexia associated with APR in chicks is not well understood. In the present study, the possible involvement of corticotrophin-releasing factor (CRF) on anorexic effects of LPS in neonatal chicks was investigated. For this aim, different doses of LPS were administrated via both intracerebroventricular (ICV) and intraperitoneal (IP) routes in order to assess its effects on chick’s food intake. Subsequently, the effect of ICV injection of astressin, a CRF receptor antagonist, on anorexia induced by ICV and IP administration of LPS was investigated. Food intake was significantly decreased following either central or systemic administration of LPS. ICV co -injection of astressin and LPS significantly diminished anorexic effects of central LPS. However, anorexia induced by peripheral LPS was not attenuated by central injection of astressin. These data indicated that the brain CRF receptors are involved in central LPS-induced anorexia in chicks.
https://ijvst.um.ac.ir/article_30307_a21d0e33a032515685dc7a60bbfb05db.pdf
2019-07-01
19
25
10.22067/veterinary.v1i11.76494
Lipopolysaccharide
Acute illness anorexia
Corticotrophin-releasing factor
Neonatal chicks
Razieh
kooshki
kooshki.r@lu.ac.ir
1
shahid bahonar university
LEAD_AUTHOR
Mehdi
Abbasnejad
mabbas@uk.ac.ir
2
shahid bahonar university
AUTHOR
Hossein
Jonaidi
hjonaidi@uk.ac.ir
3
shahid bahonar university
AUTHOR
Mohadese
Soltaninejad
mohadese.soltani.mic@gmail.com
4
shahid bahonar university
AUTHOR
Abdolhamid
Sharifimehr
sharifi.science@gmail.com
5
shahid bahonar university
AUTHOR
Manochehr
Yosoufi
m_usofi2001@yahoo.com
6
shahid bahonar university
AUTHOR
Mobin
Aghapour
bionicmobin@gmail.com
7
shahid bahonar university
AUTHOR
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51
ORIGINAL_ARTICLE
The eff ect of two Iranian viper snake; vipera albicornuta (zanjani) and vipera latifi i (lattifi i) venoms on the viability of rat bone marrow mesenchymal stem cells in vitro and in vivo
Snake venom is a complex mixture of different compounds which have potential pharmacological properties and may affect mesenchymal stem cells (MSCs). In this study we investigate the effects of two Iranian vipers; vipera albicornuta and vipera latifii crude venoms on the viability of MScs in vivo and in vitro. The cells in in vitro tests were treated with different concentrations (1, 2, 3, 4 and 5 μg/100μl) of mentioned venoms for 24, 48 and 72 hours.The cells in in vivo experiment only were treated with v. Latifii venom at the concentration of 1μg/100μl and time intervals as in vitro tests. The cell viability in in vitro experiment was assessed using MTT assay. The results of in vitro experiments showed that maximum cell viability was observed at concentrations of 1 and 2 μg/100μl of v. albicornuta and v. latifi ivenoms aft er 48 and 72 hours, respectively. The results of in vivo experiment showed that the cells treated with v. Latifii venom for 72 hours in situ have the highest proliferation rate after passages three, four and five in comparison to control. The results of this study showed that the v. albicornuta and v. latifi i venoms can affect the confluence and viability of the MSCs.
https://ijvst.um.ac.ir/article_30339_5f19309a84029f7a179e59374b9ca299.pdf
2019-07-01
27
33
10.22067/veterinary.v1i11.76565
Mesenchymal Stem Cells
venom
Viper snake
Cell culture
Bone marrow
Fatemeh
Salami
fatemeslm2020@gmail.com
1
School of Veterinary MedicineFerdowsi University of Mashhad
LEAD_AUTHOR
Fatemeh
Younesi Soltani
fatemeys.1992@gmail.com
2
Faculty of Veterinary MedicineFerdowsi University of Mashhad
AUTHOR
Amin
Tavassoli
tavassoli.am@gmail.com
3
Faculty of Veterinary MedicineFerdowsi University of Mashhad
AUTHOR
Behrooz
Fathi
behrooz048@gmail.com
4
Faculty of Veterinary MedicineFerdowsi University of Mashhad
AUTHOR
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ORIGINAL_ARTICLE
Antibacterial eff ect of Lavandula stoechas and Origanum majorana essential oils against Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli
This research examined the antimicrobial effect of Lavandula stoechas (lavender) and Origanum majorana (marjoram) essential oil against three mastitis-causing pathogens: Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. Gas chromatography-mass spectrometry (GC/MS) analysis revealed that the main components of the lavender and marjoram oils were 17-Pentatriacontene, Linalyl acetate, Eucalyptol, linalool and 3-Cyclohexene-1-ol,4-methyl-1-(1-methylethyl)-,(R)-, α-terpineol, P-cymene, respectively. Broth dilution testing was performed using autoclaved whole milk instead of broth to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of essential oils alone and in combination. In addition, time-kill assay of lavender and marjoram oils were determined in milk up to 24 h. MIC values ranged from 3.12 - 4.37% v/v and MBC between 6.25 - 8.75% v/v for the lavender. The MIC and MBC of the marjoram ranged from 0.62 - 1.87% v/v and 1.25 - 3.75% v/v, respectively. The MIC ranged from 2.5 - 5% v/v and MBC between 5 - 10% v/v for lavender + marjoram combination. In time-kill assays, the presence of lavender and marjoram oils at a sub-MIC concentration significantly reduced the bacterial population in 4, 10 and 24 h. Generally, essential oil of marjoram had greater antibacterial activity than lavender against all mastitis-causing pathogens tested and has the potential to be evaluated as an alternative or adjunct to antibiotics in the treatment of bovine mastitis.
https://ijvst.um.ac.ir/article_30373_54fd8673a7a77fc7b41f63027e724e99.pdf
2019-07-01
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10.22067/veterinary.v1i11.77679
Antibacterial activity
Lavender
Marjoram
Organic farm
samira
noori
samiranoori202020@gmail.com
1
university of gonbad kavous
AUTHOR
Reza
Rahchamani
rahchamani@gonbad.ac.ir
2
university of bonbad kavous
LEAD_AUTHOR
Javad
Bayat Kohsar
bayatkoohsar@gonbad.ac.ir
3
university of gonbad kavous
AUTHOR
fatemeh
Bahri Binabaj
f_bahri_b@gonbad.ac.ir
4
university of gonbad kavous
AUTHOR
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ORIGINAL_ARTICLE
Effect of different levels of milkweed (Calotropis persica)seed powder on the growth parameters, immunity and gut microbiota of Oncorhynchus mykiss
An 8-week feeding experiment was conducted to investigate the inclusion potential of five levels of Calotropis persica seed powder (CSP) (0, 10, 20, 30, 40 and 50 g/kg of the basal diet) in rainbow trout (Oncorhynchus mykiss) diet, in a completely randomized design. To perform the test, 3600 fries (11.5 ± 3.64 g) were treated for 56 days. According to the results, the increase in milkweed seed powder up to 40 g/kg, resulted in a significant increase in specific growth rate and intestinal lactic acid bacteria count compared to the control (p < 0.05). The best results of survival rate, feed conversion ratio, hepatosomatic and gastrosomatic indices were achieved in the treatments receiving 20, 30, 40, and 50 g/kg CSP (p < 0.05). Antibacterial activity of skin mucus, lysozyme and alkaline phosphatase showed the highest level in the 40 g/kg treatment (p < 0.05). Based on the results, the inclusion of 40 g/kg milkweed seed powder caused positive health effects and could be a suitable herbal feed additive in the rainbow trout diet.
https://ijvst.um.ac.ir/article_30413_19215a3bcd5ebe2c66d9448bd4aa6dc0.pdf
2019-07-01
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50
10.22067/veterinary.v1i11.76088
Calotropis persica
growth
immunity
Gut microbiota
Oncorhynchus mykiss
Hamidreza
Ahmadniaye Motlagh
ahmadnia@um.ac.ir
1
Ferdowsi University of Mashhad
LEAD_AUTHOR
Omid
Safari
omidsafari@um.ac.ir
2
Ferdowsi University of Mashhad
AUTHOR
Marina
Paolucci
paolucci@unisannio.it
3
University of Sannio
AUTHOR
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ORIGINAL_ARTICLE
The eff ects of extenders containing proline and glutamine on oxidative stress and motion parameters of stallion semen during cold storage
This study examined the effects of skim-milk based extenders supplemented with proline and glutamine on motility, lipid peroxidation and enzymatic antioxidant status of cooled-stored equine sperm and determined the role of seminal plasma as well. The semen was collected with artificial vagina. In experiment 1, native semen was diluted in skim-milk based extender containing 5mM glutamine and 3mM proline, stored at 5°C and analyzed at 4, 24 and 48 hours storage for motion parameters. In experiment 2, semen was centrifugated, sperm pellet resuspended in the extenders and stored at 5°C for 4 hours to determine motion parameters. The level of catalase, glutathione peroxidase activity and malondialdehyde formation was determined for all samples at 4 hours. Glutamine and proline signifi cantly preserved the percentage of motile sperm (76.5 ± 2.7 and 79.4 ± 1 vs 69 ± 1.4), increased the progressive motility of cold-stored semen (66.1 ± 2.5 and 73.7 ± 2.9 vs 56.2 ± 1.4), increased catalase activity, and decreased malondialdehyde. However these effects were disappeared after seminal plasma removal. We conclude that glutamine and proline would amplify the antioxidant activity of equine cold-stored semen and preserve its motility. This effect seems to be related to interactions with seminal plasma.
https://ijvst.um.ac.ir/article_30449_9099215dfed8e3e4bf780c039d4a00cd.pdf
2019-07-01
51
59
10.22067/veterinary.v11i1.80929
Glutamine
Proline
Cold storage
stallion semen
Antioxidant activity
Najmeh
Davoodian
davoodian.najmeh@sku.ac.ir
1
Shahrekord University of Shahrekord
LEAD_AUTHOR
Ali
Kadivar
kadivar.ali@gmail.com
2
Shahrekord University of Shahrekord
AUTHOR
Ebrahim
Ahmadi
eahmadif@yahoo.com
3
Shahrekord University of Shahrekord
AUTHOR
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