Antifungal and toxicity effects of new combined essential oils on Oncorhynchus mykiss in comparison with malachite green

Document Type : Research Articles

Authors

• Mohammad Mousavi ¹
• Seyed Saeed Mirzargar ²
• Hosseinali Ebrahimzadeh Mousavi ²
• Reza Omidbaigi ³
• Alireza Khosravi ⁴
• Alireza Bahonar ⁵

¹ 1Department of Fisheries, Faculty of Marine Natural Resources, Khoramshahr Marine Sciences and Technology University, Khoramshahr

² 2Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran

³ Department of Horticulture, Faculty of Agriculture, University of Tarbiat Modarres, Tehran

⁴ 4Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran

⁵ 5Department of Food Control & Hygen, Faculty of Veterinary Medicine, University of Tehran, Tehran

10.22067/veterinary.v4i2.9457

Abstract

Mold infection is one of the most important problems in aquaculture. Recently, administration of some chemicals such as malachite green in order to control mold infections has been limited in aquaculture. In the current decade, researchers have been more focused on using herbal extracts and essential oils in aquaculture. In this study, Minimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) of a new combination of four essential oils (Eucalyptus globulus, Mentha piperita, Salvia officinalis and Thymus vulgaris) were determined by serial dilution method. LC50 of the combined essential oils for 48 and 96 hours were then determined on Oncorhynchus mykiss fingerlings. MIC and MFC of combined essential oils were 0.025μl/ml and 0.050μl/ml for F. solani and 0.018μl/ml and 0.035μl/ml for S. parasitica, which was lower compared to malachite green. It was also 0.060μl/ml and 0.300μl/ml for F. solani and 0.045μl/ml and 0.120μl/ml for S. parasitica respectively (p

Keywords

• mold infection
• salmonid hatcheries
• Essential oil
• MIC
• MFC
• LC50

20.1001.1.2008465.2012.4.2.1.3

Main Subjects

• Toxicology

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References

Abd-Elmageed, M.A. and Hussein, B.A. (2008). Cytotoxicity and antimicrobial activity of Salvia officinalis L. flowers, Sudan Journal of Medical Sciences, 3(2), 127-132.

 

Adams, R. P.(2001). Identifications of essential oil components by Gas Chromatography/Quadrupolc Mass spectroscopy. Allured publications. Crop., Carol sream IL.

 

Al-Bayati, F. (2007). Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. Journal Ethnopharmacol, 116(3), 403-406.

 

Arndt, R.E., Wagner, E.J. and Routledge, M.D. (2001). Reducing or withholding hydrogen peroxide treatment during a critical stage of rainbow trout development: effects on eyed eggs, hatch, deformities, and fungal control. North American Journal of Aquaculture, 63, 161–166.

 

Bajpai, V. K., Rahman, A. and Kang, S. Ch. (2007). Chemical composition and anti-fungal properties of the essential oil and crude extracts of Metasequoia glyptostroboides Miki ex Hu. Industrial Crops and Products, 26, 28–35.

 

Bauer, K., Garbe, D., Surburg, H., (2001). Common fragrance and flavor materials: preparation, properties and uses. Wiley-VCH, Weinheim, 293.

 

Burt, S., (2004). Esssential oils: theirs antibacterial properties and potential applications in foods- a review, International Journal of Food Microbiology, 94, 223-253.

 

Cowan, M.M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12, 564–568.

 

Culp, S.J., Mellick, P.W., Trotter, R.W., Greenlees, K.J., Kodell, R.L. and Beland, F.A. (2006). Carcinogenicity of malachite green chloride and leucomalachite greenin B6C3F1 mice and F344 rats. Food and Chemical Toxicology, 44, 1204–1212.

 

Duarte, M.C.T., Figueira, G.M., Satoratto, A., Rehder, V.L.G. and Delarmelina, C. (2005). Anti-Candida activity of Brazilian medicinal plants, Journal of Ethnopharmacol, 97, 305–311.

 

Eghtesad, S.S., Mirzaei, H., Farzam Pour, S. and Kahnamui, S. (2009). Inhibitory Effects of endemic Thymus vulgaris and Mentha piperita Essential oils on Escherichia coli O157:H7, Research Journal of Biological Sciences, 4(3), 340-344.

 

Eloff, J.N. (1998). A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica, 64, 711-713.

 

Forneris, G., Bellardi, S., Palmegiano, G. B., Saroglia, M., Sicuro, B., Gasco, L. and Zoccarato, I. (2003). The use of ozone in trout hatchery to reduce saprolegniasis incidence. Aquaculture, 221, 157–166.

 

Hadjikhoondi, A., Aghel, N., Zamanizadeh-Nadgar, N. and Vatandoost, H. (2000). Chemical and Biological Study of Mentha spicata L. essential oil from Iran. Daru, 8 (1,2), 19-21.

 

Hussein, M.M.A., and Hatai, K. (2002). Pathogenicity of Saprolegnia species associated withoutbreaks of salmonid saprolegniosis in Japan, Fisheries Science,68, 1067–1072.

 

Iscan, G., Kirimer, N., Kurkcuoglu, M., Baser, K.H.C., and Demirci, F. (2002). Antimicrobial screening of Mentha piperita essential oils. Journal of Agricultural and Food Chemistry, 50, 3943-3946.

 

Khan, R., Islam, B., Akram, M., Shakil, Sh., Ahmad, A., Manazir Ali, S., Siddiqui, M. and Khan, A.U. (2009). Antimicrobial Activity of Five Herbal Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus of Clinical Origin. Molecules, 14, 586-597.

 

Lee, Sh. H., Chang, K. Sh., Su, M. Sh., Huang, Y. Sh. and Jang, Sh. D. (2007). Effects of some Chinese medicinal plant extracts on five different fungi. Food Control, 18, 1547–1554.

 

Mahasneh, A.M. and El-Oqlah, A.A. (1999). Antifungal activity of extracts of herbal plants used in traditional medicine of Jordan, journal of ethnopharmacology, 64, 271-276.

 

Marino, M., Bersani, C. and Comi, G. (2001). Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International Journal of Food Microbiology, 67, 187–195.

 

Mohsenzadeh, M. (2007). Evaluation of Antibacterial Activity of Selected Iranian Essential Oils against Staphylococcus and Escherichia coli in Nutrient Broth Medium. Pakistan Journal of Biological Sciences, 10(20), 3693-3697.

 

Mousavi, S.M., Mirzargar, S.S., Ebrahim Zadeh Mousavi, H.A., Omid Baigi, R., Khosravi, A. and Ahmadi, M.R. (2009). Evaluation of Antifungal Activity of New combined essential oils in comparison with malachite green on Hatching Rate in Rainbow Trout (Oncorhynchus mykiss) eggs. Canadian Journal of Fisheries and Aquatic Sciences, 4(2),103-110.

 

Musyimi, D.M. and Ogur, J.A. (2008). Comparative Assessment of Antifungal Activity of Extracts from Eucalyptus globulus and Eucalyptus citriodora, Research Journal of Photochemistry, 2(1), 35-43.

 

Nilsen, P. V. and Rios, R. (2000). Inhibition of fungal growth on bread by volatile components from spices and herbs, and the possible application in active packing, with special emphasis on mustard essential oil. International Journal of Food Microbiology, 60, 219–229.

 

Olesen, P. T., Larsen, J. Ch. and Schnipper, A. (2007). Risk assessment of malachite green and leucomalechite green found in farmed fish. Toxicology Letters, 172S, S1–S240.

 

Pinto, E., Riberio Saluerio, L., Cavaleiro, C.,Palmeria, A. and Jose Goncalves, M. (2007).In vitro susceptibility of some species of yeasts and filamentous fungi to essential oils of Salvia officinalis.Ind. Crops and products, 26,135-141.

 

Pina-Vaz, C., Rodfigues, A. G., Pinto, E., Costa-de-Oiiveira, H. S., Tavares, C., Salgueiro, L., Cavaleiro, C., Goncalves, M. and Martinez-de-oliveira, J. (2004).Antifungal activity of Thymus oils and their major compounds. Journal of the European Academy of Dermatology and Venereology, 18, 73-78.

 

Post, G. (1987). Textbook of fish health. Revised and expanded edition Ltd., TFH Publications, Inc., Neptune City, New Jersey. P: 288.

 

Rai, M.K., Qureshi, S. and Pandey, A.K. (1999). In vitro susceptibility of opportunistic Fusarium spp. to essential oils. Mycoses, 42, 97-101.

 

Segvic Klaric, M., Kosalec, I., Mastelic, J., Pieckova, E. and Pepeljnak, S. (2007). Antifungal activity of thyme (Thymus vulgaris L.) essential oil and thymol against moulds from damp dwellings. Letters in Applied Microbiology, 44, 36-42.

 

Senatore, F. (1996). Influence of harvesting time on yield and composition of the essential oil of a thyme (Thymus pulegioides L.) growing wild in Campania (Southern Italy). Journal of Agricultural and Food Chemistry, 44, 1327-1332.

 

Srivastava, S.J., Singh, N.D., Srivastava, A.K. and Sinha, R. (1995). Acute toxicity of malachite green and its effects on certainblood parameters of a catfish, Heteropneustes fossilis. Aquatic Toxicology, 31, 241-247.

 

Srivastava, Sh., Sinha, R. and Roya, D. (2004). Toxicological effects of malachite green. Aquatic Toxicology, 66, 319–329.

 

Stammati, A., Nebbia, C., Angelis, I., Giuliano Albo, A., Carletti M., Rebecchi C., Zampaglioni, F. and Dacasto, M. (2005). Effects of malachite green (MG) and its major metabolite, leucomalachite green (LMG), in two human cell linesToxicology in Vitro, 19, 853–858.

 

Stenhagen, E., Abrahamson,S., Mclafery, F. (1974). Registry of Mass spectral data. Wiley & sonsInc, New York Sudova, E., Machova, J., Svobodova, Z. and Vesely, T. (2007). Negative effects of malachite green and possibilities of its replacement in the treatment of fish eggs and fish: a review. Veterinarni Medicina, 52(12): 527–539.

 

Svobodova, Z. and Vikosoa, B. (1991). Diagnostics, Prevention and Therapy of fish diseases and toxications. Czech Pubpage: 164-217.

 

Tassou, C., Koutsoumanis, K. and Nychas, G. J. E. (2000). Inhibition of Salmonella enteritidis and Staphylococcus aureus in nutrient broth by mint essential oil. Food Research International, 33, 273–280.

 

Valero, M. and Salmeroj, M. C. (2003). Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. International Journal of Food Microbiology, 85, 73–81.

 

Velickovic, D., Randjelovic, N., Ristic, M., Velikovic, A. and Melcerovic, A. (2003). Chemical constituents and antimicrobial activity of the ethanol extracts obtained from the flower, leaf and stem of Salvia officinalis L., J.Serb.Chem.Soc.68 (1), 17–24.

 

Vidya, T.J. and Vidya, P. (2000). Antimicrobial activity of Scavon Vet cream. The Veterinarian, 24, 16.

 

Wong, P. Y. Y. and Kitts, D. D. (2002). The effects of herbal pre-seasoning on microbial and oxidative changes in irradiated beefsteaks. Food Chem., 76, 197–205.

 

Yildirim, A., Mavi, A., Oktay, M., Kara, A. A., Algur, O. F. and Bilaloggu, V. (2000). Comparison of antioxidant and antimicrobial activities of tilia (Tilia argentea), sage (Salvia triloba L.), and black tea (Camellia sinensis) extracts. Journal of Agricultural and Food Chemistry, 48, 5030–5034.